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Recombinant Macaca fascicularis Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

Recombinant Macaca fascicularis Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

^{In Stock}

Code	CSB-MP5104MOV
Abbreviation	Recombinant Cynomolgus monkey IL2RA protein, partial (Active)
MSDS	MSDS Datasheet
Size	$102
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/ml can bind Macaca fascicularis IL2 (CSB-MP640916MOVd9). The EC₅₀ is 88.60-116.8 ng/mL. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/ml can bind Human IL2 (CSB-MP011629HU). The EC₅₀ is 0.8151-1.087 ng/mL. Biological Activity Assay Activity Macaca fascicularis IL2 (CSB-MP640916MOVd9) captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay (WeSPRTM 200). Biological Activity Assay The purity of IL2RA was greater than 95% as determined by SEC-HPLC
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Product Details

Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Macaca fascicularis IL2 (CSB-MP640916MOVd9). The EC50 is 88.60-116.8 ng/mL.
②Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Human IL2 (CSB-MP011629HU). The EC50 is 0.8151-1.087 ng/mL.
③Macaca fascicularis IL2 (CSB-MP640916MOVd9) captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay (WeSPRTM 200).

Target Names

IL2RA

Uniprot No.

H6WS54

Alternative Names

Interleukin-2 receptor subunit alpha

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

22-238aa

Target Protein Sequence

ELCDDDPPKITHATFKAVAYKEGTMLNCECKRGFRRIKSGSPYMLCTGNSSHSSWDNQCQCTSSAARNTTKQVTPQPEEQKERKTTEMQSQMQLADQVSLPGHCREPPPWENEATERIYHFVVGQTVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGETEPSQFPGEEEPQASPDGLPESETSRLVTTTDFRIQTEVAATMETFIFTTE

Mol. Weight

26.1 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The alpha subunit of the interleukin-2 receptor governs high-affinity IL-2 binding and T-cell activation, making it a critical target in immunotherapy and transplant rejection research. This mammalian-expressed construct spanning residues 22–238 demonstrates quantified binding to both cynomolgus and human IL-2, with EC50 values of 88.60–116.8 ng/mL and 0.8151–1.087 ng/mL respectively in functional ELISA, while surface plasmon resonance confirms a 31.3 nM affinity constant for the cynomolgus IL-2 interaction. The cross-species reactivity supports comparative primate studies and translational antibody development, providing a suitable basis for therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody validation assays. Dual-method purity verification by SDS-PAGE and SEC-HPLC (both >95%) combined with endotoxin levels below 1.0 EU/μg aligns with standards expected in ligand-binding assays and SPR-based affinity characterization workflows.