Recombinant Macaca fascicularis Nectin cell adhesion molecule 2 (NECTIN2), partial (Active)

NECTIN2 is a cell-adhesion molecule involved in cell-cell interactions, making its functional characterization critical for studying intercellular communication and adhesion-related signaling pathways. This recombinant Macaca fascicularis Nectin cell adhesion molecule 2 (NECTIN2), partial (Active) (CSB-MP5776MOV) is a mammalian cell-expressed protein spanning amino acids 32–360, featuring a C-terminal 10xHis-tag for purification and detection, with a molecular weight of 36.9 kDa. Functional activity is demonstrated by its binding ability in a functional ELISA, where immobilized Macaca fascicularis NECTIN2 at 2 μg/mL binds the Anti-NECTIN2 recombinant antibody (CSB-RA835690MA1HU) with an EC50 of 1.011–1.193 ng/mL, confirming specific interaction. Purity exceeds 95% as determined by both SDS-PAGE (Tris-Glycine discontinuous reduced gel with 5% enrichment and 15% separation) and SEC-HPLC, while endotoxin levels are below 1.0 EU/μg (measured by the LAL method). These attributes support its use in cell-adhesion mechanism studies, related signaling pathway investigations, antibody binding assays, and as a tool for exploring NECTIN2-mediated intercellular interactions. The high purity, low endotoxin, and His-tag facilitate reliable quantification and integration into various biochemical and cell-based workflows.