Recombinant Mouse 14-3-3 protein sigma (Sfn)

Code CSB-YP021135MO
MSDS
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Source Yeast
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Code CSB-EP021135MO
MSDS
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Source E.coli
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Code CSB-EP021135MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021135MO
MSDS
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Source Baculovirus
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Code CSB-MP021135MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sfn
Uniprot No.
Alternative Names
Sfn; Mkrn314-3-3 protein sigma; Stratifin
Species
Mus musculus (Mouse)
Expression Region
1-248
Target Protein Sequence
MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VKEYREKVET ELRGVCDTVL GLLDSHLIKG AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADSAGEEGG EAPEEPQS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Gene References into Functions
  1. Loss of 14-3-3-sigma sensitizes mice to chemically-induced skin carcinogenesis. Therefore, 14-3-3-sigma may indeed represent a mediator of tumor suppression in the skin. PMID: 27409835
  2. 14-3-3sigma stabilizes a complex of soluble actin and intermediate filament to enable breast tumor invasion. PMID: 24067649
  3. these data provides the first evidence that 14-3-3 sigma is a Smad3-dependent target gene of TGF-beta1. PMID: 23375543
  4. 14-3-3sigma plays an important role in regulating mouse embryonic stem cell proliferation by binding and sequestering phosphorylated GSK-3beta and enhancing Wnt-signaled GSK-3beta inactivation. PMID: 22768254
  5. We show that mice heterozygous for the 14-3-3sigma mutation have severe defects in hair shaft differentiation, resulting in destruction of the hair shaft during morphogenesis. PMID: 22377760
  6. Data show that endogenous 14-3-3sigma protein formed a complex with FOXO1 protein. PMID: 21205887
  7. 14-3-3 sigma is required for TGF-beta1-mediated growth inhibition in mouse mammary epithelial cells. PMID: 20082218
  8. 14-3-3 sigma is needed for normal hair growth PMID: 20237493
  9. 14-3-3sigma plays a critical role in retaining epithelial polarity PMID: 20439433
  10. 14-3-3sigma is critical for regulating corneal epithelial proliferation and differentiation by regulating Notch signaling activity. PMID: 20100467
  11. 14-3-3 can mediate the relocalization of nuclear ligands by several mechanisms that ensure complete sequestration of the bound 14-3-3 complex in the cytoplasm. PMID: 11864996
  12. Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth. PMID: 12075357
  13. Keratin 10 -/- mice exhibited induction of c-Myc, cyclin D1, 14-3-3sigma and wound healing keratins K6 and K16. PMID: 12077355
  14. 14-3-3 sigma appears to have a positive feedback effect on p53 activity PMID: 14517281
  15. P4 regulates the phosphorylation status of 14-3-3sigma through a PKG-dependent pathway, and 14-3-3sigma plays a central and essential role in maintaining the viability of spontaneously immortalized granulosa cell. PMID: 15286034
  16. mutation in stratifin is responsible for the repeated epilation phenotype PMID: 16200063
  17. 14-3-3sigma is a crucial regulator for skin proliferation and differentiation. A single nucleotide insertion in the Sfn coding region results in a truncated protein linked with phenotypes of Er-heterozygous and -homozygous mice. PMID: 16239341
  18. 14-3-3sigma stabilizes p27 Kip1 by inhibiting the activity of PKB/Akt PMID: 16532026
  19. IKKalpha protects the 14-3-3sigma locus from hypermethylation, which serves as a mechanism of maintaining genomic stability in keratinocytes. PMID: 17643371
  20. These observations suggest that, in addition to an increased gene copy number and upregulation of EGR2 and CITED1, an elevated erbB2 transcript level involves the loss of 14-3-3sigma, which sequesters a key transcriptional regulator of the erbB2 promoter. PMID: 17938205

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Subcellular Location
Cytoplasm. Nucleus. Secreted.
Protein Families
14-3-3 family
Tissue Specificity
Expressed in the basal layer of skin epithelium and in outer root sheath of hair follicle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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