Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rnasel

Uniprot No.

Q05921

Alternative Names

Rnasel; Rns4; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; EC 3.1.26.-; Ribonuclease 4; Ribonuclease L; RNase L

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover. Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.

Gene References into Functions

Pref-1 mRNA is a novel substrate of RNase-L. PMID: 27831565
RNAse L stimulates fibroblast migration. PMID: 26517238
Thus, activation of RNase L does not require mouse hepatitis virus virus-induced interferon but rather correlates with adequate levels of basal Oas gene expression, maintained by basal interferon signaling. PMID: 26739051
Thus, RNA cleavage events catalyzed by RNase L are required for optimal inflammasome activation during viral infections. PMID: 25816776
cellular functions of RNase L through protein-protein interactions in the spleen for immune response in mammals PMID: 24780566
Data indicate that deficiency of 2-5A-dependent RNase L (RNase L) in resulted in a significant delay of diabetes onset. PMID: 25287058
RNase L contributes to innate immunity through regulating macrophage functions. PMID: 24324683
By targeting the effector enzyme of this antiviral pathway, L* potently inhibits RNase L, underscoring the importance of this enzyme in innate immunity against Theiler's virus. PMID: 23825954
RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer. PMID: 23567782
These studies highlight novel roles of RNase L in cigarette smoke plus virus induced inflammation, tissue remodeling, apoptosis, and cytokine elaboration PMID: 23913960
RNase L activation during mouse hepatitis virus infection is cell type specific and correlates with relatively high levels of expression of oligoadenylate synthetase genes, which are necessary for an effective RNase L antiviral response. PMID: 23698313
The results suggest that RNase L is not an evolutionarily-conserved host defense mechanism to counteract retroviruses in vivo. PMID: 23725696
These findings highlight RNase L as an essential regulator of adipogenesis via the regulation of CHOP10 mRNA. PMID: 22441668
RNase L induces autophagy via c-Jun N-terminal kinase and double-stranded RNA-dependent protein kinase signaling pathways PMID: 23109342
results indicate a novel function of RNase L as an inducer of autophagy that affects viral yields PMID: 22875977
Mouse hepatitis virus Ns2 cleaves 2',5'-oligoadenylate, the product of 2',5'-oligoadenylate synthetase, to prevent activation of the cellular endoribonuclease RNase L and consequently block viral RNA degradation. PMID: 22704621
sunitinib treatments prevent antiviral innate immune responses mediated by RNase L and PKR. PMID: 21636578
Endoribonuclease L (RNase L) regulates the myogenic and adipogenic potential of myogenic cells PMID: 19851509
JNK and RNase L function in an integrated signaling pathway during the IFN response that leads to elimination of virus-infected cells through apoptosis PMID: 14570908
results indicate that the RNase-L-dependent decrease in mtDNA-encoded mRNA transcript levels occurs through a decrease in the half-life of mt-mRNA, and that RNase-L may play a role in the stability of mt-mRNA PMID: 15522195
The RNase L pathway is critical for in vitro interferon-alpha-mediated islet cell resistance from coxsackievirus serotype B4 infection and replication. PMID: 15661870
RNAse L is required for the antiviral activity of the interferon (IFN)-beta transgene during acute ocular herpes simplex virus type 1 infection. PMID: 16002711
The data indicate that West Nile Virus genomic RNA is susceptible to RNase L cleavage and that RNase L plays a role in the cellular antiviral response to flaviviruses. PMID: 16501108
Data suggest that the primary role of double-stranded RNA binding by the NS1A protein in virus-infected cells is to sequester dsRNA away from RNAse L. PMID: 16627618
Restriction mapping and structure of the 5' flanking region of the RNase L gene. PMID: 17091337
Plays role in senescence that may contribute to its tumor suppressive function and to the enhanced longevity of RNase-L-deficient mice. PMID: 17130839
RNase L does not play a significant role in control of HSV-2 infection in vivo PMID: 17157346
results indicate that RNase L has an essential role in the innate antiviral immune response that relieves the requirement for direct sensing of non-self RNA PMID: 17653195
a unique role for RNase-L in the antibacterial response that is mediated through multiple mechanisms PMID: 19075243
support a model in which the RNase-L-mediated degradation of ribosomal protein mRNAs inhibits translation, and may contribute to its antiproliferative, senescence inducing and tumor suppressor activities. PMID: 19411840
These data demonstrate a novel protective role for RNase L in viral induced CNS encephalomyelitis PMID: 19798426

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Subcellular Location

Cytoplasm. Mitochondrion.

Protein Families

Protein kinase superfamily

Tissue Specificity

Expressed in spleen, thymus, lung, testis, kidney, liver and heart.

Database Links

KEGG: mmu:24014

STRING: 10090.ENSMUSP00000083385

UniGene: Mm.259254

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Recombinant Mouse 2-5A-dependent ribonuclease (Rnasel), partial

Product Details

Customer Reviews and Q&A

Target Background

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