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Recombinant Mouse 5-aminolevulinate synthase, nonspecific, mitochondrial (Alas1)

Recombinant Mouse 5-aminolevulinate synthase, nonspecific, mitochondrial (Alas1)

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Code	CSB-EP844984MO
MSDS	MSDS
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Alas1

Uniprot No.

Q8VC19

Research Area

Cardiovascular

Alternative Names

Alas1; 5-aminolevulinate synthase; nonspecific; mitochondrial; ALAS-H; EC 2.3.1.37; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

57-642aa

Target Protein Sequence

QVKETPPANEKEKTAKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMHAVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKIDEKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAGGTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRNSRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAYFSGMSKMVSAQA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

72.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

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Target Background

Gene References into Functions

RNAi-mediated silencing of hepatic Alas1 effectively prevents and treats the induced acute attacks in acute intermittent porphyria mice. PMID: 24821812
Both ALAS1 mRNA and protein content were induced in diabetic animals, accompanied by decreased Akt phosphorylation and increased nuclear FOXO1, PGC-1alpha and FOXO1-PGC-1alpha complex levels. PMID: 22070747
active site functional asymmetry extends to chimeric ALAS/AONS proteins, which while having a different oligomeric state, exhibit different rates of product release from the two ALAS and two AONS active sites due to the created intermolecular strain PMID: 21600186
Egr-1 regulates the transcriptional repression of mouse delta-aminolevulinic acid synthase 1 by heme PMID: 21044876
Mice bearing a homozygous knock-in allele were lethal by embryonic day 8.5, demonstrating that ALAS-N is essential for early embryogenesis. PMID: 20015225
Ser-254 is critical for formation of a competent catalytic complex by coupling succinyl-CoA binding to enzyme conformational equilibria PMID: 19917609
identified a drug-responsive enhancer in the ALAS1 gene PMID: 12881517
Histidine-282 impacts a variety of ALAS functions, including substrate and pyridoxal 5'-phosphate (PLP)-binding and catalysis. PMID: 17469798
5-aminolevulinate synthase operates under the stereoelectronic control predicted by Dunathan's hypothesis PMID: 17485466
The findings show that a B2 SINE retrotransposon can contribute to the regulation of ALAS1 and SINEs in 5'-UTR regions contribute to inter-individual differences in gene expression. PMID: 18929534