Recombinant Mouse Actin, Cytoplasmic domain 1(Actb),partial

Code CSB-RP076754m
Size US$1675
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Actb
Uniprot No. P60710
Research Area Others
Alternative Names ActbActin; cytoplasmic 1; Beta-actin) [Cleaved into: Actin; cytoplasmic 1; N-terminally processed]
Species Mus musculus (Mouse)
Source E.coli
Expression Region 11-375aa
Target Protein Sequence DNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 67.7kDa
Protein Length Partial
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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 Q&A
Q:

Can you please provide the following information about your ACTB Recombinant Protein (cat#: CSB-RP076754m):
1)Please provide updated related informations
2)What is the buffer and concentrations this protein is provided in?
3)What is the concentration of your current lot?

A:
Very nice to receive your inquiry.
Recombinant Mouse Actin, cytoplasmic 1(Actb),partial
CSB-RP076754m
Expression Region: 11-375aa; Partial.
Tag information:N-terminal GST-tagged
Sequence:

DNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF


The concentration range is 0.1-2mg/ml and default buffer is Tris-based buffer,50% glycerol, if you have any demand for the buffer or concentration, please remark your requirements when placing the order.
We can prepare the protein according to your demand.

Target Background

Function
(From Uniprot)
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. Data, including data from studies using knockout mice, suggest that Actb and Actg1 are enriched in isolated membrane preparations from skeletal muscle which represent interface between mitochondria and sarco-endoplasmic reticulum; these structures appear to be important in signaling, mitochondrial dynamics, and muscle relaxation. (Actb = beta actin cytoplasmic 1; Actg1 = actin gamma cytoplasmic 1) PMID: 29265728
  2. Essential nucleotide- and protein-dependent functions of Actb/beta-actin. PMID: 30012594
  3. These results suggest a a novel genome-wide mechanism where the polymerase-associated beta-actin synergizes with NM1 to coordinate permissive chromatin with Pol I transcription, cell growth, and proliferation. PMID: 27127100
  4. Two actin isoforms, beta- and gamma-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. PMID: 27840001
  5. Melatonin treatment for 1 h increased monomeric actin and decreased polymeric actin. PMID: 28209510
  6. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes beta-actin mRNA and affects cell migration. PMID: 25588836
  7. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
  8. Actin binding proteins associated with stress fiber or focal adhesion formation are overexpressed in the beta-actin knock-out cells and many of these contain CH-, LIM- or EFh- domains. PMID: 24123846
  9. Absence of beta-actin severely hampers formation of the peripheral nervous system, due to defective neural crest migration. PMID: 24409333
  10. Data indicate that ectopic phosphatidylinositol 3,4,5-triphosphate (PIP3) elevation alone activated membranes to form actin-based structures whose behavior was similar to that of growth-cone-like "waves". PMID: 23951027
  11. this work demonstrated that dendritic beta-actin messenger RNA and ribosomes are in a masked, neuron-specific form. PMID: 24458642
  12. chorein interacts with beta-adducin and beta-actin. PMID: 24129186
  13. RNA polymerase II, glyceraldehyde-3-phosphate dehydrogenase (Gapdh), and hypoxanthine phophoribosyltransferase (Hprt) show a more stable gene expression as candidate genes than do ribosomal 18s and Actb. PMID: 22735032
  14. The findings suggested that local translation of beta-actin in growth cones of motoneurons is regulated by laminin signalling and that this signalling is disturbed in survival of motoneuron protein-deficient motoneurons. PMID: 22810847
  15. Data suggest that in beta-actin knockout cells other actins compensate for beta-actin in cell migration process. PMID: 22448045
  16. Data indicate that Ubc and Ywhaz were best correlated for B cells and lymphocytes, whereas Ubc and Gapdh were the best combination for non-B cells, and Actb and Hprt1 were the least stably expressed genes for B cells and non-B cell. PMID: 22884776
  17. the zipcode-binding protein 1 transports beta-actin mRNA to the focal adhesion compartment, it dwells for minutes, associating its localization with motility through the formation of stable connections between adhesions and newly synthesized actin filaments PMID: 22948660
  18. These data show that thymosinbeta4 and beta-actin are over-expressed during nurse cell formation upon T. spiralis infection and may be involved in nurse cell formation along with other actin-binding proteins. PMID: 22305657
  19. novel roles for beta-actin in promoting complex CNS tissue architecture PMID: 22403730
  20. Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes. PMID: 21778137
  21. These data support an essential role for beta-actin in regulating cell migration and gene expression through control of the cellular G-actin pool PMID: 21900491
  22. Perturbing the dystrophin and beta actin interaction results in quadriceps myopathy. PMID: 21325027
  23. Nuclear beta-actin controls growth arrest of epithelial cells. PMID: 21172822
  24. Results demonstrate overlapping developmental roles but unique post-developmental functions for beta-actin and gamma-actin in maintaining hair cell stereocilia. PMID: 20976199
  25. Data show that exposure to hyperoxia resulted in significant increases in eNOS-beta-actin association, eNOS activity, and protein tyrosine nitration in the lungs. PMID: 20826796
  26. arginylated gamma-actin, unlike beta-, was highly unstable and selectively ubiquitinated and degraded in vivo; instability was regulated by differences in nucleotide coding sequence between the actin isoforms, which conferred different translation rates PMID: 20847274
  27. HnRNP-R-mediated axonal beta-actin mRNA translocation plays an essential physiological role for axon growth and presynaptic differentiation. PMID: 20167579
  28. Dystrophin and utrophin bind actin through distinct modes of contact PMID: 16478721
  29. beta-actin was arginylated in vivo to regulate actin filament properties, beta-actin localization, and lamella formation in motile cells PMID: 16794040
  30. Data show that beta-actin characteristic markers of brush borders, become abnormally distributed along these atypical basolateral membranes in gal3(-/-) mice. PMID: 18211959
  31. The cytoplasmic beta-actin (Actb) gene generates two alternative transcripts terminated at tandem polyA sites. PMID: 18835850
  32. Ischemia-mediated aggregation of the actin cytoskeleton is one of the major initial events resulting in ischemia-reperfusion injury. PMID: 19095765

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Subcellular Location Cytoplasm, cytoskeleton
Protein Families Actin family
Database Links

KEGG: mmu:11461

STRING: 10090.ENSMUSP00000098066

UniGene: Mm.328431

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