Recombinant Mouse Alpha-actinin-4 (Actn4), partial

Code CSB-YP001244MO
MSDS
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Source Yeast
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Code CSB-EP001244MO
MSDS
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Source E.coli
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Code CSB-EP001244MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001244MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP001244MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Actn4Alpha-actinin-4; Non-muscle alpha-actinin 4
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Gene References into Functions
  1. Focal segmental glomerulosclerosis Actn4 mutation promotes podocyte detachment through maladaptation to periodic stretch. PMID: 29378953
  2. ACTN4 is a transcriptional coactivator of NF-kappaB. PMID: 25411248
  3. sLZIP negatively regulates skeletal muscle differentiation via interaction with ACTN4. PMID: 24375477
  4. the C-terminal tail of alpha-actinin-4 is essential for its function in cell migration and adhesion to substratum. PMID: 23466492
  5. Dexamethasone mey protect actin cytoskeleton stabilization from adriamycin-induced podocyte injury via alpha-actinin-4. PMID: 22804863
  6. anti-dsDNA Fab may enhance F-actin formation by the proprietary modification of alpha-actinin-4 PMID: 22929799
  7. Together, these results indicate that alpha-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS. PMID: 21940706
  8. Results suggest that ACTN4 is essential for maintaining normal spreading, motility, cellular and nuclear cross-sectional area, and contractility of lung fibroblasts by maintaining the balance between transcellular contractility and cell-adhesion. PMID: 21085685
  9. newly developed mouse model of ACTN4-associated FSGS suggests a cause-and-effect relationship between actin cytoskeleton dysregulation by mutant alpha-actinin-4 and the deterioration of the nephrin-supported slit diaphragm complex. PMID: 12707390
  10. nonsarcomeric alpha-actinin-4 is involved in the regulation of cell movement PMID: 12782671
  11. ACTN4 has a role promoting tumorigenicity and regulating cell motility PMID: 15048094
  12. FSGS-associated Actn4 impairs cytoskeletal dynamcs in podocytes. PMID: 16837921
  13. alpha-actinin-4 interacts with integrins and strengthens the podocyte-GBM interaction thereby stabilizing glomerular architecture and preventing disease PMID: 17082197
  14. The different genetic defects in Actn4 produce a spectrum of glomerular morphologic lesions depending on the specific combination of normal and/or defective alleles. PMID: 18185509
  15. Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation. PMID: 18332111
  16. Alpha-actinin-4 K256E triggers several metabolic abnormalities, which may lead to glomerular epithelial cell injury and glomerulosclerosis. PMID: 19640905

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Subcellular Location
Nucleus. Cytoplasm. Cell junction. Cytoplasm, cytoskeleton, stress fiber. Cytoplasm, perinuclear region.
Protein Families
Alpha-actinin family
Database Links
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