Recombinant Mouse Annexin A1(Anxa1)

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Code CSB-EP001836MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Anxa1
Uniprot No. P10107
Research Area Others
Alternative Names Anxa1; Anx1; Lpc-1; Lpc1Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Species Mus musculus (Mouse)
Source E.coli
Expression Region 2-346aa
Target Protein Sequence AMVSEFLKQARFLENQEQEYVQAVKSYKGGPGSAVSPYPSFNVSSDVAALHKAIMVKGVDEATIIDILTKRTNAQRQQIKAAYLQENGKPLDEVLRKALTGHLEEVVLAMLKTPAQFDADELRGAMKGLGTDEDTLIEILTTRSNEQIREINRVYREELKRDLAKDITSDTSGDFRKALLALAKGDRCQDLSVNQDLADTDARALYEAGERRKGTDVNVFTTILTSRSFPHLRRVFQNYGKYSQHDMNKALDLELKGDIEKCLTTIVKCATSTPAFFAEKLYEAMKGAGTRHKALIRIMVSRSEIDMNEIKVFYQKKYGISLCQAILDETKGDYEKILVALCGGN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 42.6kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity
Gene References into Functions
  1. ANXA1 is a novel GnRH target gene in gonadotropes. ANXA1 also may be a target of local GnRH in peripheral tissues and may have a different role than that of ANXA5 PMID: 29213013
  2. ANXA1 can be phosphorylated by PKC and is subsequently translocated to the nucleus of BV-2 microglial cells after oxygen glucose deprivation/reoxygenation, resulting in the induction of pro-inflammatory cytokines. PMID: 27426034
  3. Our data indicate that hepatic overexpression of ANX1 and ANX2 inhibits APAP-induced expansion of liver injury PMID: 27451051
  4. The present findings shed light on the role of GILZ in the mechanism of induction of Anxa1 by GCs. As Anxa1 is an important protein for the resolution of inflammatory response, GILZ may represent a new pharmacologic target for treatment of inflammatory diseases. PMID: 28373208
  5. these data indicate that AnxA1 is actively expressed during L. braziliensis infection. PMID: 28289158
  6. Data, including data from studies using knockout mice, suggest cAMP-elevating agents increase levels of AnxA1; this is involved in role of cAMP in resolving acute inflammation (here, ability of cAMP-elevating agents to treat LPS-induced neutrophilic pleurisy). These results reinforce hypothesis that AnxA1 acts in multiple cell types (neutrophils, macrophages) and at multiple levels to promote resolution of inflammation. PMID: 28655761
  7. confirmed the ability of Plg/Pla to both promote efferocytosis and override the prosurvival effect of LPS via annexin A1. PMID: 28320709
  8. this study shows that AnxA1 plays a crucial role in the context of acute gouty inflammation by promoting timely resolution of inflammation PMID: 27995621
  9. Suggest protective role for Annexin A1 in arterial neointima formation in atherosclerosis-prone mice. PMID: 28062503
  10. ANXA1 restores Abeta42-induced blood brain barrier disruption through inhibition of RhoA-ROCK signaling pathway PMID: 27633771
  11. It is proposed that the interactions between ANXA1 and GATA-3 may provide clues to understand the immunosuppression and have potential as new therapeutic targets in immunotherapy after sepsis. PMID: 27833268
  12. AnxA1, is related to the efficiency of the infliximab treatment in experimental colitis, constituting a potential biomarker of therapeutic efficacy PMID: 27343762
  13. these data highlight AnxA1 as a novel determinant of neutrophil maturation and the mechanisms behind blood neutrophil homing to BM via the CXCL12/CXCR4 pathway. PMID: 26892496
  14. We conclude that ANX-A1 is an important regulator of mast cell reactivity to compound 48/80 exerting a negative feedback effect through a mechanism that depends at least partly on the FPR receptor PMID: 26803520
  15. muscle-contraction-induced calpain-dependent ANXA1 fragmentation has a wound-healing effect on damaged cells. This suggested that not the intact form but rather fragmented ANXA1 is a contraction-induced myokine PMID: 26458561
  16. Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regeneration of Injured Muscles PMID: 26667898
  17. Data show that endothelial annexin A1 from apolipoprotein AI knockout mice was decreased in comparison to that from wild type mice. PMID: 27012521
  18. Anxa1 mRNA and protein is highly expressed in mesenchymal stromal cells (MSCs). Anxa1 KO MSCs cannot enhance glucose stimulated islet insulin secretion. PMID: 26470781
  19. pan antagonist of AnxA1 receptors exacerbated the colitis outcome in TNFR1-/- mice, supporting the pivotal role of AnxA1 in the early recovery PMID: 26386311
  20. knock-out mice are more susceptible to Mycobacterium tuberculosis infection PMID: 25533809
  21. critical regulator of Th17 cell driven autoimmune diseases such as uveitis PMID: 25619792
  22. annexin A1- formyl peptide receptor 2 pathway mediated the insulin resistance of skeletal muscle, as well as systemic insulin sensitivity. PMID: 25616869
  23. Functional role of ANXA1 in tumorigenesis was established in invasion assay where knocking down ANXA1 in murine mammary tumor cell line 168FARN showed lower invasive capability. PMID: 25124533
  24. AnxA1 and Fpr2 have a critical role in the manifestation of adrenal insufficiency in this LPS-induced model, through regulation of cholesterol ester storage PMID: 25818588
  25. The annexin A1 fragment Ac2-26 counteracts conformational activation and clustering of integrins on myeloid cells evoked by CCL5, CCL2, and CXCL1 through inhibiting activation of the small GTPase Rap1. PMID: 25520364
  26. these data indicate that annexin1 is essential in immunity to Mtb infection and mediates the power of DC efferocytosis and cross-presentation. PMID: 25562320
  27. Data (including data from knockout mice) suggest that mobilization of neutrophils during stress requires expression of ANXA1 and activation of glucocorticoid receptor in neutrophils. PMID: 25184992
  28. AnxA1 participates in a paradigm in which malignant prostate epithelial cells that are not cancer stem cells are induced to gain cancer stem cell-like properties. PMID: 24464914
  29. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
  30. findings identify ANXA1 as a proinvasive protein in melanoma that holds promise as a potential prognostic marker and therapeutic target PMID: 24997993
  31. Macrophage-derived AnxA1 plays a functional role in modulating hepatic inflammation and fibrogenesis during nonalcoholic steatohepatitis progression. PMID: 24668763
  32. Data (including data from knockout mice) suggest AnxA1 limits inflammatory response in central (cerebral cortex) and peripheral (mesenteric) microvasculature; estrogen acts independent of AnxA1 to protect cerebral but not mesenteric microvasculature. PMID: 22897118
  33. Data show an increase of extracellular Annexin A1 (ANXA1) starting from the initial phases of skeletal muscle cell differentiation. PMID: 23144744
  34. AX1 can exert diverse, fusogen-specific effects on cell-cell fusion, functioning as an extracellular mediator of differentiation-dependent membrane fusion or as an intracellular promoter of postfusion pore expansion and syncytium formation. PMID: 24648446
  35. The present studies tested the hypothesis that neonatal hyperoxic exposure induces deficits in CCSP expression that are associated with persistent alterations in lung SCGB and ANXA1 expression. PMID: 24187664
  36. ANXA1 plays an important role in TLR activation, leading to an augmentation in the type 1 IFN antiviral cytokine response. PMID: 24048896
  37. In mice, annexin 1 prevented the development of inflammatory dendritic cells and suppressed the cellular immune response against the model antigen ovalbumin expressed in apoptotic cells. PMID: 23638088
  38. we suggest that MIF counter-regulates Dexamethasone-induced annexin 1 expression PMID: 23777345
  39. Results indicate that the AnxA1/FPR2 system has an important role in effecting the resolution of cerebral inflammation in sepsis and may provide a novel therapeutic target. PMID: 22964301
  40. T-cell-expressed AnxA1 functions to attenuate T-cell-driven inflammatory responses via T-cell-intrinsic effects on intracellular signaling, proliferation, and Th1/Th17 cytokine release. PMID: 23267026
  41. revealed annexin A1 to be an effective regulator in tumor stroma and suggested a mechanism that annexin A1 affects tumor development and metastasis PMID: 23077482
  42. ANXA1(-/-) mice exhibit significantly increased BBB permeability as a result of disrupted interendothelial cell tight junctions, essentially related to changes in the actin cytoskeleton, which stabilizes tight and adherens junctions. PMID: 23277546
  43. a novel epithelial FPR1/NOX1-dependent redox signaling pathway that promotes mucosal wound repair. PMID: 23241962
  44. data raise the possibility that a reduction in plasma AnxA1 may contribute to the chronic inflammatory phenotype observed in human obesity PMID: 23038751
  45. The data showed that annexin A1 exerts an inhibitory effect on COX-2 expression in the macula densa. ANXA1 may be a novel intrinsic modulator of renal juxtaglomerular regulation by inhibition of PGE2 synthesis. PMID: 22791338
  46. Data suggest that Annexin 1 (ANXA1) plays a protective role in DNA damage and modulates cell adhesion and motility, indicating its potential role in cancer initiation as well as progression in breast carcinoma. PMID: 22511458
  47. AnxA1 is an endogenous determinant for the therapeutic efficacy of Dexamethasone in inflammatory arthritis. PMID: 22562975
  48. AnxA1 mediates natural and glucocorticoid-induced resolution of inflammation with profound effects on neutrophil apoptosis. PMID: 22493082
  49. Cleavage of ANXA1 during mast-cell activation causes proinflammatory reactions by increasing the phosphorylation of cytosolic phospholipase A2 (cPLA2) and production of eicosanoids. PMID: 22539796
  50. ANXA1 is an important regulatory factor in the development of allergic disease and dysregulation of its expression can lead to pathological changes which may affect disease progression. PMID: 22092555
  51. Expression of AnxA1 by resident macrophages is a critical determinant for neutrophil clearance in the bone marrow. PMID: 21957127
  52. counter-regulates bleomycin-induced lung fibrosis PMID: 22011168
  53. Annexin A1 binds to phagosomes in the presence of Ca(2+) and facilitates the F-actin-phagosomes interaction. PMID: 21245195
  54. comparison of binding of a panel of glycans and sulfated glycans to annexin A1; kinetics of binding of heparan sulfate and heparin to annexin A1 PMID: 21370880
  55. Strikingly, forced AnxA1 expression in metastatic mouse and human mammary carcinoma cells reversed epithelial-to-mesenchymal transition and abolished breast cancer metastasis. PMID: 20821804
  56. Data demonstrate that inhibition of the cystic fibrosis transmembrane conductance regulator reduced cell-associated annexin A1 by promoting release of the protein in microparticles. PMID: 20489160
  57. We show that ANXA1 is fundamental for brain homeostasis PMID: 20962261
  58. Propose a novel mechanism to explain the antiinflammatory actions of cromones on neutrophil trafficking invloving annexin A1. PMID: 20558817
  59. Results suggest for the first time that annexin A1 plays a role in myogenic differentiation. PMID: 20578244
  60. A role for endogenous AnxA1 in regulating both positive and negative selection of the TCR repertoire. PMID: 20139728
  61. AnxA1 knockdown in invasive basal-like breast cancer cells reduced the number of spontaneous lung metastasis, whereas additional expression of AnxA1 enhanced metastatic spread. PMID: 20308542
  62. results show a direct correlation between Annexin A1 expression and severity of experimental autoimmune encephalomyelitis (EAE); suggest that Annexin A1 null mice have an impaired capacity to develop experimental autoimmune encephalomyelitis. PMID: 19912648
  63. Mice lacking Anx-1 gene have increased COX-2 AND PLA2, abnormal leukocyte adhesion molecule expression. In inflammation, Anx-1(-/-) mice have exaggerated response and partial or complete resistance to anti-inflammatory effects of glucocorticoids. PMID: 12516535
  64. endogenous regulatory autocoids such as lipoxin A(4) and annexin 1-derived peptides function to disengage adherent cells during cell-cell interactions PMID: 12560218
  65. annexin I is externalized from pituitary folliculo-stellate cells by an ABC transporter and the ATP binding cassette transporter A1 is a likely candidate PMID: 12586783
  66. annexins A1 and A2 bind to dysferlin PMID: 14506282
  67. Annexin-1 exerts endogenous antiinflammatory effects on antigen-induced arthritis via regulation of cytokine gene expression, and also mediates antiinflammatory actions of dexamethasone. PMID: 15022342
  68. phosphorylation of annexin 1 at Ser5 by TRPM7 kinase may modulate function of annexin 1 PMID: 15485879
  69. These results suggest that annexin A1 may be a key protein involved in CF pathogenesis especially in relation to the not well defined field of inflammation in CF. PMID: 16014420
  70. ABCA1 has a role in ANXA1 export PMID: 16601136
  71. Serine-27 phosphorylation is essential for the translocation of ANXA1 across the cell membrane. PMID: 16720734
  72. ANXA1 supports aspects of adipose tissue mass and alters the sensitivity of epididymal adipose tissue to catecholamines, glucocorticoids, and lipopolysaccharide PMID: 16835395
  73. Data indicate that ANXA1 deficiency is associated with gender-specific changes in corticotroph number and structure, via direct actions of ANXA1 and/or indirect changes in factors such as IL-6. PMID: 17026533
  74. ANXA1 is a regulator of adrenocortical size and corticosterone secretion. PMID: 17158208
  75. the actions of ANXA1 in the pituitary gland are independent of Fpr1 but may involve other FPR family members PMID: 17218541
  76. Loss of annexin 1 is associated with abnormal craniofacial bone development PMID: 17405164
  77. ANXA1 protein modulates nociceptive processing at the spinal level, by reducing synthesis of PGE2 by modulating cPLA2 and/or COX activity. The analgesic activity of methylprednisolone is mediated by spinal ANXA1. PMID: 18469846
  78. Endogenous AnxA1 plays a role in the protective and reparative properties of intestinal mucosal epithelium during the inflammatory process. PMID: 18802107
  79. Results suggest that multiple inflammation pathways centered on annexin-A1 might become activated at different stages of the chronic inflammatory response, including the delayed and pro-resolving phase. PMID: 18827967
  80. To characterize ANXA1 involvement in melanoma B16 dissemination, we reduced ANXA1 protein level by siRNA and observed a significant decrease of B16Bl6 cell invasion through Matrigel coated chambers. PMID: 18952200
  81. Anx-1-deficient dendritic cells acquire a mature phenotype during differentiation. PMID: 19029200
  82. endogenous AnxA1 is an essential effector of endogenous anti-inflammation PMID: 19095957
  83. Expression of the magnesium transporter TRPM7 was significantly elevated in the vasculature of low intracellular magnesium mice, whereas expression of a TRPM7 downstream target, the anti-inflammatory molecule annexin-1, was reduced PMID: 19103997
  84. glucocorticoids regulate the expression and function of annexin A1 in opposing ways in innate and adaptive immune cells to mediate the resolution of inflammation. Review. PMID: 19104500
  85. The activity of Anxa1 relies on prostaglandin E(2) (PGE(2)) induction that brings about apoptosis in thymocytes. PMID: 19244132
  86. ANXA1 is a key inhibitor of macrophage cytokine expression and GILZ is a mechanism of the anti-inflammatory effects of ANXA1. PMID: 19553536
  87. annexin A1-dependent inhibition of adrenocorticotrophin release involves the enhancement of actin polymerization to prevent exocytosis via formyl peptide receptor and Rho kinase signaling pathways. PMID: 19625660
  88. novel pro-angiogenic functions for AnxA1 in vascular endothelial cell sprouting, wound healing, and tumor growth and metastasis PMID: 19805119

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Subcellular Location Nucleus, Cytoplasm, Cell projection, cilium, Basolateral cell membrane, Lateral cell membrane, Cell membrane, Peripheral membrane protein, Apical cell membrane, Membrane, Peripheral membrane protein, Early endosome, Cytoplasmic vesicle membrane, Peripheral membrane protein, Endosome membrane, Peripheral membrane protein, Secreted, Secreted, extracellular space, Cell membrane, Peripheral membrane protein, Extracellular side, Secreted, exosome, Cytoplasmic vesicle, secretory vesicle lumen, Cell projection, phagocytic cup
Protein Families Annexin family
Tissue Specificity Detected in lung (PubMed:12475898, PubMed:17384087). Detected at the apical membrane of airway epithelial cells (PubMed:17384087). Detected in intestinal epithelial cells (PubMed:18802107). Detected in skeletal muscle (PubMed:14506282). Detected in prosta
Database Links

KEGG: mmu:16952

STRING: 10090.ENSMUSP00000025561

UniGene: Mm.248360

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