Recombinant Mouse Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 (Arap3), partial

Code CSB-YP823193MO
MSDS
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Source Yeast
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Code CSB-EP823193MO
MSDS
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Source E.coli
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Code CSB-EP823193MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823193MO
MSDS
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Source Baculovirus
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Code CSB-MP823193MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arap3
Uniprot No.
Alternative Names
Arap3; Centd3; Drag1; Kiaa4097; Arf-GAP with Rho-GAP domain; ANK repeat and PH domain-containing protein 3; Centaurin-delta-3; Cnt-d3; Dual specificity Rho- and Arf-GTPase-activating protein 1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts preferentially on ARF5 and on RHOA.
Gene References into Functions
  1. Deletion of Arap3 in osteoblasts and mesenchymal stromal cells using Prx1-Cre resulted in no discernable phenotypes in hematopoietic development or hematopoietic stem cells homeostasis in adult mice. PMID: 25542002
  2. Arap3 is dysregulated in a mouse model of hypotrichosis-lymphedema-telangiectasia and regulates lymphatic vascular development. PMID: 24163130
  3. We report here the first characterization of the role of ARAP3 in murine neutrophils PMID: 21490342
  4. PI3K signaling through the dual GTPase-activating protein ARAP3 is essential for developmental angiogenesis. PMID: 20978237
  5. ARAP3 has a role in integrin-mediated tyrosine kinase signalling pathways controlling Rho GTPases and cell spreading PMID: 15546919
  6. the Nck adapters are needed for signaling to Rho GTPases and actin dynamics downstream of the PDGFbeta receptor PMID: 18765673

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Note=Cytoplasmic, and associated with F-actin-rich membrane ruffles and lamellipodia.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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