Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Cd207

Uniprot No.

Q8VBX4

Research Area

Signal Transduction

Alternative Names

Cd207; Clec4kC-type lectin domain family 4 member K; Langerin; CD antigen CD207

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

63-331aa

Target Protein Sequence

QAVFYPRLMGKILDVKSDAQMLKGRVDNISTLGSDLKTERGRVDDAEVQMQIVNTTLKRVRSQILSLETSMKIANDQLQILTMSWGEVDSLSAKIPELKRDLDKASALNTKVQGLQNSLENVNKLLKQQSDILEMVARGWKYFSGNFYYFSRTPKTWYSAEQFCISRKAHLTSVSSESEQKFLYKAADGIPHWIGLTKAGSEGDWYWVDQTSFNKEQSRRFWIPGEPNNAGNNEHCANIRVSALKCWNDGPCDNTFLFICKRPYVQTTE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

34.7 kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Constructing a plasmid that codes for the Mouse Cd207 protein (63-331aa) is the initial step to yield the recombinant Mouse Cd207 protein. The plasmid is then transferred into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. A N-terminal 6xHis tag is fused to the protein. The affinity purification is used to purify the protein, and SDS-PAGE analysis is carried out to verify the presence and assess the purity of the protein. The protein possesses a purity exceeding 90%.

Target Background

Function

Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.

Gene References into Functions

Binding analysis between purified long and short forms of langerin and a series of keratin sulfate disaccharide components shows that the binding critically requires the 6-sulfation of Gal and that the long form of langerin displays higher affinity than the short form. PMID: 29631057
Bacterial Polysaccharide Specificity of the Pattern Recognition Receptor Langerin Is Highly Species-dependent PMID: 27903635
Data show that Candida albicans and Candida tropicalis preferentially localized in (and persisted within) a sub- population of Peyer's patch Langerin-positive dendritic cells. PMID: 26386376
Langerin is expressed by the XCR1(+) "DC1" population of mice. PMID: 25516751
Langerin+ dermal dendritic cells appear to be site- and strain-specific PMID: 24629018
model of epicutaneous ovalbumin sensitization inducing an inflammatory skin resembling atopic dermatitis (AD) to explore the role of CD207 in the pathogenesis of AD PMID: 23117802
Langerin-positive dendritic cells appear to be the predominant, though not exclusive, population responsible for in vivo transport of anti-DEC-205 antibodies from the skin to the draining lymph nodes in steady state and inflammation. PMID: 22291181
Data demonstrated that CD207(+) CD8alpha(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors. PMID: 21630253
The normal corneal epithelium is endowed with CD11c(+) Langerin+ cells that are LCs, whereas the stroma is endowed with a separate population of (non-LC) Langerin+ DCs. PMID: 21482644
Langerhan cells and (Langerin(+)) dermal dendritic cells thus seem to have a redundant function in regulating contact hypersensitivity PMID: 20703247
molecular cloning and expression of Langerin transcripts in dendritic cells PMID: 11978773
Developmentally regulated expression of Langerin isoforms provides a mechanism by which Langerin involvement in antigen uptake and processing could be regulated. PMID: 15191546
CD207 has a role in maintaining Birbeck granules but not in Langerhans cell function PMID: 15601833
Langerin may have an unanticipated role in cell-matrix interactions that modulate LC development, localization, or function PMID: 16557233
CD207 antigens manifest a high degree of colocalization around the nucleus of the Langerhans cells. PMID: 16941665
langerin(+) DCs patrol the dermis in the steady state and migrate to the skin DLNs charged with skin antigens PMID: 18086862
dermal Langerin(+) dendritic cells (DCs) are radiosensitive and display a distinct cell surface phenotype PMID: 18086865
Depletion of langerin(+) cells impaired intestinal IgA Ab responses after Topical transcutaneous immunization PMID: 18354155
Langerin(+) cells do promote T cell responses to skin Ags PMID: 18354196
In a knockin-mouse model of the immune response against Leishmania major parasites, langerin-expressing dendritic cells are, in general, dispensable for an efficient adaptive immune response against L. major parasites. PMID: 19124720
Despite a marginal zone location, CD207(+) DCs lacked identifying molecules for 3 different types of macrophages, localized in proximity and, in contrast to macrophages, marginal zone DCs were poor scavengers of soluble and particulate substrates PMID: 19168629
Langerhans cells(LC) and langerin(+) dermal dendritic cells(DC) represent distinct DC subsets that have specialized functions and that LC are important immunoregulatory cells PMID: 19218433

Hide All

Subcellular Location

Membrane; Single-pass type II membrane protein.

Tissue Specificity

Expressed by Langerhans cells. Expressed in dendritic cells and by scattered cells in lymph nodes and spleen. Also detected in some non-lymphoid tissues such as lung, liver and heart.

Database Links

KEGG: mmu:246278

STRING: 10090.ENSMUSP00000040746

UniGene: Mm.136079

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Code	CSB-EP837695MO
Abbreviation	Recombinant Mouse Cd207 protein, partial
MSDS	MSDS Datasheet
Size	US$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse C-type lectin domain family 4 member K (Cd207), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage