Recombinant Mouse C-type lectin domain family 4 member K (Cd207), partial

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Code CSB-EP837695MO
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Cd207
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Cd207; Clec4kC-type lectin domain family 4 member K; Langerin; CD antigen CD207
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
63-331aa
Target Protein Sequence
QAVFYPRLMGKILDVKSDAQMLKGRVDNISTLGSDLKTERGRVDDAEVQMQIVNTTLKRVRSQILSLETSMKIANDQLQILTMSWGEVDSLSAKIPELKRDLDKASALNTKVQGLQNSLENVNKLLKQQSDILEMVARGWKYFSGNFYYFSRTPKTWYSAEQFCISRKAHLTSVSSESEQKFLYKAADGIPHWIGLTKAGSEGDWYWVDQTSFNKEQSRRFWIPGEPNNAGNNEHCANIRVSALKCWNDGPCDNTFLFICKRPYVQTTE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
34.7 kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Constructing a plasmid that codes for the Mouse Cd207 protein (63-331aa) is the initial step to yield the recombinant Mouse Cd207 protein. The plasmid is then transferred into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. A N-terminal 6xHis tag is fused to the protein. The affinity purification is used to purify the protein, and SDS-PAGE analysis is carried out to verify the presence and assess the purity of the protein. The protein possesses a purity exceeding 90%.

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Target Background

Function
Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.
Gene References into Functions
  1. Binding analysis between purified long and short forms of langerin and a series of keratin sulfate disaccharide components shows that the binding critically requires the 6-sulfation of Gal and that the long form of langerin displays higher affinity than the short form. PMID: 29631057
  2. Bacterial Polysaccharide Specificity of the Pattern Recognition Receptor Langerin Is Highly Species-dependent PMID: 27903635
  3. Data show that Candida albicans and Candida tropicalis preferentially localized in (and persisted within) a sub- population of Peyer's patch Langerin-positive dendritic cells. PMID: 26386376
  4. Langerin is expressed by the XCR1(+) "DC1" population of mice. PMID: 25516751
  5. Langerin+ dermal dendritic cells appear to be site- and strain-specific PMID: 24629018
  6. model of epicutaneous ovalbumin sensitization inducing an inflammatory skin resembling atopic dermatitis (AD) to explore the role of CD207 in the pathogenesis of AD PMID: 23117802
  7. Langerin-positive dendritic cells appear to be the predominant, though not exclusive, population responsible for in vivo transport of anti-DEC-205 antibodies from the skin to the draining lymph nodes in steady state and inflammation. PMID: 22291181
  8. Data demonstrated that CD207(+) CD8alpha(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors. PMID: 21630253
  9. The normal corneal epithelium is endowed with CD11c(+) Langerin+ cells that are LCs, whereas the stroma is endowed with a separate population of (non-LC) Langerin+ DCs. PMID: 21482644
  10. Langerhan cells and (Langerin(+)) dermal dendritic cells thus seem to have a redundant function in regulating contact hypersensitivity PMID: 20703247
  11. molecular cloning and expression of Langerin transcripts in dendritic cells PMID: 11978773
  12. Developmentally regulated expression of Langerin isoforms provides a mechanism by which Langerin involvement in antigen uptake and processing could be regulated. PMID: 15191546
  13. CD207 has a role in maintaining Birbeck granules but not in Langerhans cell function PMID: 15601833
  14. Langerin may have an unanticipated role in cell-matrix interactions that modulate LC development, localization, or function PMID: 16557233
  15. CD207 antigens manifest a high degree of colocalization around the nucleus of the Langerhans cells. PMID: 16941665
  16. langerin(+) DCs patrol the dermis in the steady state and migrate to the skin DLNs charged with skin antigens PMID: 18086862
  17. dermal Langerin(+) dendritic cells (DCs) are radiosensitive and display a distinct cell surface phenotype PMID: 18086865
  18. Depletion of langerin(+) cells impaired intestinal IgA Ab responses after Topical transcutaneous immunization PMID: 18354155
  19. Langerin(+) cells do promote T cell responses to skin Ags PMID: 18354196
  20. In a knockin-mouse model of the immune response against Leishmania major parasites, langerin-expressing dendritic cells are, in general, dispensable for an efficient adaptive immune response against L. major parasites. PMID: 19124720
  21. Despite a marginal zone location, CD207(+) DCs lacked identifying molecules for 3 different types of macrophages, localized in proximity and, in contrast to macrophages, marginal zone DCs were poor scavengers of soluble and particulate substrates PMID: 19168629
  22. Langerhans cells(LC) and langerin(+) dermal dendritic cells(DC) represent distinct DC subsets that have specialized functions and that LC are important immunoregulatory cells PMID: 19218433

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Subcellular Location
Membrane; Single-pass type II membrane protein.
Tissue Specificity
Expressed by Langerhans cells. Expressed in dendritic cells and by scattered cells in lymph nodes and spleen. Also detected in some non-lymphoid tissues such as lung, liver and heart.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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