Recombinant Mouse Cyclin-dependent kinase 4(Cdk4)

Code CSB-YP005065MO
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Source Yeast
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Code CSB-EP005065MO
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Source E.coli
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Code CSB-EP005065MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005065MO
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Source Baculovirus
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Code CSB-MP005065MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Cdk4
Uniprot No. P30285
Alternative Names Cdk4; Crk3Cyclin-dependent kinase 4; EC 2.7.11.22; CRK3; Cell division protein kinase 4; PSK-J3
Species Mus musculus (Mouse)
Expression Region 2-303
Target Protein Sequence AATRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGAAGGGLP VSTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDRDIKVT LVFEHIDQDL RTYLDKAPPP GLPVETIKDL MRQFLSGLDF LHANCIVHRD LKPENILVTS NGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR EVSLPRGAFA PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKEES DAE
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Gene References into Functions
  1. CDK4/CDK6-dependent activation of DUB3 regulates cancer metastasis through SNAIL1. PMID: 28067227
  2. Study examined proliferation rates of cortical progenitor cells in mice lacking individual cdk proteins or combinations thereof. Results provide evidence that concomitant loss of cdk4 and cdk6 leads to reduced proliferation rates specifically in basal progenitor cells in both the dorsal and ventral forebrain. Cdk4-/-;Cdk6-/- double mutant progenitor cells in the dorsal telencephalon exhibit accelerated cell cycle exit. PMID: 29570951
  3. These results indicate that p18 blocks reprogramming by targeting Cdk4/6-mediated cell cycle regulation. PMID: 27484146
  4. Activation of cdk4 triggers NAFLD. PMID: 27373160
  5. This novel mechanism explains how CDK4 promotes anabolism by blocking catabolic processes (FAO) that are activated by AMPK. PMID: 29053957
  6. PD 0332991 (PD), an FDA-approved selective inhibitor of cyclin-dependent kinase 4/6 (CDK4/6), prevents radiation-induced lethal intestinal injury in mice. Treating mice with PD or a structurally distinct CDK4/6 inhibitor prior to radiation blocked proliferation and crypt apoptosis and improved crypt regeneration. PMID: 27701148
  7. Cdk4 protein PMID: 26975029
  8. The results demonstrate a unique CDK4-mediated mitochondrial communication that allows cells to sense environmental genotoxic stress and boost mitochondrial homeostasis by enhancing phosphorylation and activation of MnSOD. PMID: 25578653
  9. a crucial role of RXRa in suppression of UVB-induced melanomas in the context of driver mutations such as activated CDK4(R24C/R24C) or oncogenic NRAS(Q61K) and altered expression of p53 and PTEN PMID: 25189354
  10. Cdk4 and Cdk6 cooperate in hematopoietic tumor development and suggest a role for Cdk6 in sequestering INK4 proteins away from Cdk4. PMID: 25157181
  11. CDK4 seems to be expressed as multiple proteins that respond differently to different shRNAs, and have previously unrecognized functions at the S-G 2/M phases of the cell cycle via mechanisms independent of binding to CCND and RB. PMID: 24091631
  12. CDK4 deficiency markedly accelerated lymphoma development in the Emu-Myc transgenic model of B lymphoma PMID: 24614102
  13. Cdk4- and Arf-mutant mice exhibited similar profiles, the Trp53(F/F) ::Tyr-Cre(ER)::Tyr-NRAS melanomas were strikingly different, showing relative down-regulation of melanocyte-related genes, and up-regulation of genes related to neural differentiation. PMID: 23724991
  14. CDK4 amplification is associated with drug sensitivity in glioblastoma. PMID: 22711607
  15. CDK4-mediated regulation of cell functions via c-Jun phosphorylation and AP-1 activation PMID: 21559334
  16. Cdk4 has a direct role in angiogenesis in vivo and may be an important drug target to reduce or prevent angiogenesis during intestinal tumor formation. PMID: 20603602
  17. Cdk4 and its downstream transcription factor E2f1 regulate pancreas development prior to and during the secondary transition. PMID: 21490060
  18. Data show that Cdk4 plays a previously unrecognized role in thymocyte maturation and adhesion, but is not required for thymocyte proliferation. PMID: 21150327
  19. Results demonstrate that primary and metastatic melanomas developing spontaneously in Hgf-Cdk4(R24C) mice effectively evade cellular immune surveillance. PMID: 20649939
  20. Data show that in the absence of keratinocytic RXRalpha, in combination with mutant Cdk4, cutaneous melanoma was generated that metastasized to lymph nodes in a bigenic mouse model. PMID: 20629968
  21. Cdk4-R24C mutation contributes to the development of melanoma in Tyr-HRas mice. PMID: 20703083
  22. Overexpression of Cdk4 is associated with non-small cell lung carcinoma. PMID: 20609353
  23. analysis of a subset of glioblastoma multiforme likely to respond to CDK4/6 inhibition PMID: 20534551
  24. conclude that Cdk4 not only promotes beta-cell replication, but also facilitates the activation of beta-cell progenitors in the ductal epithelium. In addition, we show that Cdk4 controls beta-cell mass by recruiting quiescent cells to enter the cell cycle PMID: 20084282
  25. CDK4/6 inhibition is a potent mediator of cytostasis and that RB loss can be readily compensated for in the context of both hepatoma cell lines and liver tissue. PMID: 20100483
  26. cyclin-dependent kinase 4 and 6 inhibition arrests the growth of glioblastoma multiforme intracranial xenografts PMID: 20354191
  27. C/EBPalpha enhances the formation of cdk4-ubiquitin conjugates and induces degradation of cdk4 through a proteasome-dependent pathway. As a result, the half-life of cdk4 is shorter and protein levels of cdk4 are reduced in cells expressing C/EBPalpha. PMID: 11867521
  28. Explant-induced reactivation of herpes simplex virus occurs in neurons expressing nuclear cdk4 PMID: 12097586
  29. cyclin D-cdk4 activity modulates the subnuclear localization and interaction of MEF2 with coactivator GRIP-1 during skeletal muscle differentiation PMID: 12130539
  30. Cdk4 deficiency resulted in a 98% reduction in the number of skin tumors but did not affect normal keratinocyte proliferation PMID: 12163365
  31. Cdk4 is essential for immortalization, and suppression of Cdk4 could be a prospective strategy to recruit cells with inactive Arf/p53 pathway to senescence. PMID: 12435633
  32. calcineurin/NFAT pathway negatively regulates the expression of cyclin-dependent kinase 4 (CDK4) PMID: 12453415
  33. Inhibition of this activity enhances translation of p27kip1 in quiescent Rb-negative cells. PMID: 12566456
  34. several important differences between cdk4 and cdk6 that highlight unique functional roles for these cyclin-dependent kinases PMID: 12861051
  35. Cdk4 is not only involved in controlling proliferation of specific cell types but may play a wider role in establishing homeostatic cell numbers PMID: 12917627
  36. oocytes regulators amounts and localization undergo dramatic changes as the oocytes grow, meiotically mature, and transit into embryogenesis and CDK4, CDK6, cyclin D2, and p27 appear in unusual, most likely posttranslationally modified, forms PMID: 13679319
  37. P27Kip1 and p21Cip1 are not required for the formation of active D cyclin-cdk4 complexes PMID: 14517297
  38. deregulated cyclin D:cdk4/6 complexes and pRB perform nonoverlapping functions in vivo and provide a cellular mechanism that accounts for the low incidence of RB inactivation in cancers such as melanoma PMID: 14630948
  39. CDK4 has a role in mediate the transduction of different cell growth and proliferation signals with pINK4c PMID: 15254233
  40. Results show that cyclin-dependent kinase 4 (CDK4) mediates the proliferative and oncogenic activities of Myc in vivo through a mechanism that involves the sequestration of specific CDK inhibitors. PMID: 15314163
  41. Cdk4 and Cdk6 are not essential for cell cycle entry PMID: 15315761
  42. Cdk4 is dispensable for prenatal development of the pituitary or proliferation of other non-endocrine tissues but indispensable specifically for postnatal proliferation of somato/lactotrophs PMID: 15456744
  43. Cdk4, and its activator, cyclinD1, plays critical roles in the delayed death component of ischemic/hypoxic stress by regulating the tumor suppressor retinoblastoma protein. PMID: 16166266
  44. Cdk4 inhibition impairs adipocyte differentiation and function. Disruption of cdk4 or activating mutations in cdk4 in primary mouse embryonic fibroblasts results in reduced and increased adipogenic potential, respectively, of these cells PMID: 16213226
  45. There is a differential requirement for cyclin D1-CDK4/6 kinase activity in development versus tumorigenesis PMID: 16413468
  46. The continued presence of CDK4-associated kinase activity is required to maintain breast tumorigenesis. PMID: 16413469
  47. Mutation of BARA/LIN-9 restores the expression of E2F target genes in CDK4 null Mouse Embryo Fibroblasts, indicating that the wild-type protein plays a role in the expression of genes required for the G1/S transition. PMID: 16730350
  48. Mutant CDK4 dramatically increased the number and the growth kinetics of carcinogen-induced primary melanomas in the skin and promoted the growth of spontaneous metastases in lymph nodes and lungs. PMID: 16877364
  49. The Cdk4R24C mutation opens two avenues: when expressed specifically in beta cells, it provides a new potential strategy for beta cell regeneration in autoimmune diabetes, but its expression in the immune repertoire exacerbates autoimmunity. PMID: 18178859
  50. co-overexpression of KIT/CDK4 is a potential mechanism of oncogenic transformation in some BRAF/NRAS wild-type melanomas PMID: 18632627

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Subcellular Location Cytoplasm. Nucleus. Nucleus membrane.
Protein Families Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links

KEGG: mmu:12567

STRING: 10090.ENSMUSP00000006911

UniGene: Mm.6839

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