Recombinant Mouse D site-binding protein (Dbp)

Code	CSB-YP717147MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP717147MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP717147MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP717147MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP717147MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Dbp

Uniprot No.

Q60925

Alternative Names

DbpD site-binding protein; Albumin D box-binding protein; Albumin D-element-binding protein

Species

Mus musculus (Mouse)

Expression Region

1-325

Target Protein Sequence

MARPLSDRTP GPLLLGGPAG APPGGGALLG LRSLLQGNSK PKEPASCLLK EKERKATLPS APVPGPGLET AGPADAPSGA VSGGGSPRGR SGPVAGPSLF APLLWERTLP FGDVEYVDLD AFLLEHGLPP SPPPPGGLSP APSPARTPAP SPGPGSCSSS SPRSSPGHAP ARATLGAAGG HRAGLTSRDT PSPVDPDTVE VLMTFEPDPA DLALSSIPGH ETFDPRRHRF SEEELKPQPI MKKARKVQVP EEQKDEKYWS RRYKNNEAAK RSRDARRLKE NQISVRAAFL EKENALLRQE VVAVRQELSH YRAVLSRYQA QHGTL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.

Gene References into Functions

constitutive Dbp expression plays an important role in mediating hepatic dysfunction under chronic kidney disease. DBP was significantly decreased in 5/6Nx model mice. PMID: 26728457
Dbp plays a well-established role in circadian rhythms. PMID: 23450268
we demonstrate that D site albumin promoter binding protein/thyrotroph embryonic factor/hepatic leukemia factor triple knockout mice develop cardiac hypertrophy and left ventricular dysfunction associated with a low blood pressure PMID: 20686175
natural body temperature rhythms can affect circadian gene expression PMID: 12372249
mice deficient for all three PAR bZip proteins (DBP,HLF,& TEF) are highly susceptible to generalized spontaneous and audiogenic epilepsies, frequently lethal. Pyridoxal kinase is a target gene of PAR bZip proteins in both liver and brain PMID: 15175240
Dbp transcription cycle is paralleled by binding of BMAL1 and CLOCK to multiple extra- and intragenic E boxes. PMID: 16474407
The Dbp protein control the expression of many enzymes and regulators involved in detoxification and drug metabolism, such as cytochrome P450 enzymes, carboxylesterases, and constitutive androstane receptor (CAR). PMID: 16814730
Data show p38 MAPK- and calcium-dependent TNFR1-mediated transient increase of the negative regulator Per1 and an independent decrease of Dbp. PMID: 19625730