Recombinant Mouse DNA replication licensing factor MCM7 (Mcm7)

Code CSB-YP737046MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP737046MO
MSDS
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Source E.coli
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Code CSB-EP737046MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP737046MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP737046MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Mcm7; Cdc47; Mcmd7DNA replication licensing factor MCM7; EC 3.6.4.12; CDC47 homolog
Species
Mus musculus (Mouse)
Expression Region
2-719
Target Protein Sequence
ALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV DLDDIAEDDP ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD VYIEHRLMME QRSRDPGAVR NPQNQYPSEL MRRFELYFRG PSSSKPRVIR EVRADSVGKL LTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH SDQVPVGNIP RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV KKALLLLLVG GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ TARTQRPADV IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE YEELNVWQVN TSRTRITFV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Gene References into Functions
  1. Unique pattern of ORC2 and MCM7 localization during DNA replication licensing in the mouse zygote. PMID: 22674395
  2. role of subunits in DNA helicases activity PMID: 12207017
  3. cyclin D1/CDK4 complexes play a direct role in altering an inhibitory RB.MCM7 complex possibly allowing for setting of the origin in preparation for DNA replication PMID: 12519773
  4. MCM7 may be a highly informative biomarker for cervical cancer. PMID: 14678972
  5. Accumulation and dynamics of Mcm7 are described during oogenesis and the first embryonic cell cycle. PMID: 17554680
  6. cellular DNA replication factor MCM7 is involved in prostate cancer (CaP) and MCM7 gene expression was reduced by green tea catechins PMID: 18521193

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Subcellular Location
Nucleus. Chromosome.
Protein Families
MCM family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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