Recombinant Mouse Desmoplakin (Dsp), partial

Code CSB-YP007208MO
MSDS
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Source Yeast
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Code CSB-EP007208MO
MSDS
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Source E.coli
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Code CSB-EP007208MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007208MO
MSDS
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Source Baculovirus
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Code CSB-MP007208MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dsp
Uniprot No.
Alternative Names
Dsp; Desmoplakin; DP
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
Gene References into Functions
  1. Deletion of Dsp under the transcriptional regulation of the CSPG4 locus led to phenotype resembling the human cardiocutaneous syndromes. PMID: 29018034
  2. Conditional heterozygous deletion of Dsp in mice led to increased fibroadipogenesis in the heart and mild cardiac dysfunction. PMID: 27121621
  3. Our data suggest that endurance exercise accelerates arrhythmogenic ventricular cardiomyopathy pathogenesis in transgenic DSP mice PMID: 26545710
  4. GSK3- and PRMT-1-dependent modifications of desmoplakin control desmoplakin-cytoskeleton dynamics. PMID: 25733715
  5. These data indicate a function of desmoplakin in motor nerve regeneration by linking N-cadherin to intermediate filaments in regenerating motor axons. PMID: 25496840
  6. A loss of desmoplakin expression led to an abnormal distribution of Cx43 and Nav1.5, while scrapeloading dye/transfer revealed a decrease in dye transfer in DSP siRNAtreated cells. PMID: 23877755
  7. the desmosomal protein desmoplakin is not essential for cell adhesion in the intestinal epithelium. PMID: 22238362
  8. Loss of desmoplakin expression and resultant disruption of desmosomal adhesion can promote increased local tumor invasion independent of adherens junction status. PMID: 20862307
  9. desmoplakin has a role in capillary formation PMID: 15190119
  10. desmocollins show a higher degree of similarity to the classical cadherins, such as E-cadherin, than to the desmogleins. PMID: 15346771
  11. Data show suppression of desmoplakin expression leads to nuclear localization of plakoglobin and a 2-fold reduction in canonical Wnt/beta-catenin signaling through Tcf/Lef1 transcription factors. PMID: 16823493
  12. DSP expression in cardiomyocytes is crucial for maintaining cardiac tissue integrity, and DSP abnormalities result in ARVD/C by cardiomyocyte death, changes in lipid metabolism, and defects in cardiac development. PMID: 16917092
  13. Results demonstrate a differentiation-specific rearrangement of the microtubule cytoskeleton in epidermis, and defines an essential role for desmoplakin in the process. PMID: 17227889

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Subcellular Location
Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane.
Protein Families
Plakin or cytolinker family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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