Recombinant Mouse Dihydroorotate dehydrogenase (quinone),mitochondrial (Dhodh), partial

The preparation of Recombinant Mouse Dhodh protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This Dhodh was expressed in E.coli at and fused with N-terminal 6xHis tag. According to SDS-PAGE, the purity turns out to be 85%+.

Dhodh is a key enzyme in de novo pyrimidine biosynthesis. the expression level of dihydroorotate dehydrogenase has been reported to be elevated in various types of malignant tumors and its tumor-promoting effect was considered to relate to its pyrimidine synthesis function. Dhodh is the rate-limiting enzyme in the uridine monophosphate (UMP) biosynthetic pathway and mostly located in the inner membrane of mitochondria, catalyzing the transformation of dihydroorotate to orotate dependent of Mg2+ and ATP. Mutations of Dhodh have been associated with various genetic diseases. For example, Dhodh G202A, R346W causes deficient protein stability and R135C impairs the enzymatic activity, which are linked to Miller syndrome. Dhodh polymorphism was reported to be linked with rheumatoid arthritis and lung cancer as well. Additionally, enhanced activity of DHODH has been implicated as a biomarker of malignant tumor including gastric cancer and skin cancer.