Recombinant Mouse G2/mitotic-specific cyclin-B1(Ccnb1)

Code CSB-YP004806MO
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Source Yeast
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Code CSB-EP004806MO
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Source E.coli
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Code CSB-EP004806MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004806MO
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Source Baculovirus
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Code CSB-MP004806MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ccnb1
Uniprot No. P24860
Alternative Names Ccnb1; Ccn-2; Ccnb1-rs13; Cycb; Cycb1G2/mitotic-specific cyclin-B1
Species Mus musculus (Mouse)
Expression Region 1-430
Target Protein Sequence MALRVTRNTK INAENKAKVS MAGAKRVPVT VTAASKPGLR PRTALGDIGN KVSEELQARV PLKREAKTLG TGKGTVKALP KPVEKVPVCE PEVELAEPEP EPELEHVREE KLSPEPILVD NPSPSPMETS GCAPAEEYLC QAFSDVILAV SDVDADDGAD PNLCSEYVKD IYAYLRQLEE EQSVRPKYLQ GREVTGNMRA ILIDWLIQVQ MKFRLLQETM YMTVSIIDRF MQNSCVPKKM LQLVGVTAMF IASKYEEMYP PEIGDFAFVT NNTYTKHQIR QMEMKILRVL NFSLGRPLPL HFLRRASKVG EVDVEQHTLA KYLMELSMLD YDMVHFAPSQ IAAGAFCLAL KILDNGEWTP TLQHYLSYSE DSLLPVMQHL AKNVVMVNCG LTKHMTVKNK YAASKHAKIS TLAQLNCTLV QNLSKAVTKA
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Gene References into Functions
  1. The results show that Cyclin B1 is essential for mitosis in mammalian cells and that its spatial localization has a crucial role in triggering mitosis at the correct time, even when Cdk1 can be regulated by the antagonistic Wee1-Myt1-Cdc25 pathways. PMID: 29074707
  2. Results indicate that CCNB1 is critically required for the proliferation of gonocytes and spermatogonia but may be redundant in meiosis of spermatocytes in mouse spermatogenesis. PMID: 29072697
  3. Results provide evidence that cdc2/cyclin B1 kinase activation was synchronous with the initial appearance of cytoskeletal lesions in mouse with Niemann-Pick disease type C. PMID: 29058287
  4. Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis. PMID: 27693251
  5. timing of Ccnb1 mRNA translation in mouse oocytes is dependent on the presence of transcripts with different 3' untranslated regions PMID: 28808066
  6. AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. PMID: 25349079
  7. Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PMID: 24415942
  8. Cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression. PMID: 24746669
  9. sticky siRNAs against survivin and cyclin B1 efficiently blocks growth of established subcutaneaous B16-F10 tumors as well as formation and dissemination of melanoma lung metastases. PMID: 23835136
  10. Inhibitions of Aurora B and Cyclin-dependent kinase 1 activity in vertebrate cells also have opposite effects on the timing of abscission. PMID: 23948252
  11. It was concluded that insensitivity of mouse germinal vesicle oocytes to cyclohexidine was due to the presence of sufficient cyclin B1, and that cyclin B1 level in such oocytes was maintained by an equilibrium between synthesis and degradation. PMID: 22444557
  12. Data show nuclear accumulation of cyclin B1 with a subsequent premature increase in G2/M kinase activity in Nipa-/- spermatocytes. PMID: 22696294
  13. Data show that Cyclin B1 is synchronously degraded in whole oocytes and oocyte halves with a single bivalent PMID: 22125605
  14. Data show that protein expression of cyclins D1 and B1 and cyclin-dependent kinases 2 and 4 was downregulated upon LXR activation. PMID: 21398609
  15. Sustained spindle-assembly checkpoint response requires de novo transcription and translation of cyclin B1. PMID: 20927403
  16. Results demonstrate that the transcriptional activity of HOX11 is regulated by phosphorylation of Thr247 in a cell cycle-specific manner and that this phosphorylation modulates the expression of the target gene, cyclin B1. PMID: 20846384
  17. Linker histone binding to NASP controls the ability of HSPA2 to activate CDC2 for CDC2/cyclin B1 complex formation. PMID: 19553603
  18. Nuclear accumulation is necessary for cyclin B1-dependent apoptosis. This is consistent with the idea that localization of cyclin B1 is among the factors determining the cellular decision to undergo apoptosis in response to DNA damage. PMID: 12424202
  19. findings show that in the regenerating liver the circadian clock controls the expression of cell cycle-related genes that in turn modulate the expression of active Cyclin B1-Cdc2 kinase, a key regulator of mitosis PMID: 12934012
  20. homologue disjunction in mouse oocytes is dependent on proteolysis of the separase inhibitor securin and cyclin B1 PMID: 14593421
  21. demonstrated an expression pattern in preimplantation embryos opposite to a somatic G2/M cyclin, with peak levels in G1 PMID: 15214576
  22. the ubiquitin-proteasome pathway plays important roles in oocyte meiosis resumption, spindle assembly, polar body emission, and pronuclear formation, probably by regulating cyclin B1 degradation and MAPK/p90rsk phosphorylation PMID: 15327787
  23. Cyclin B1 suppression is a critical step in the mechanism by which farnesyl transferase inhibitors trigger apoptosis and robust antitumor efficacy PMID: 15548709
  24. cyclin B1, phosphorylated cyclin B1 and p34cdc2 have similar distributions at some stages but different localizations at other stages during oocyte meiotic maturation and fertilization PMID: 15984162
  25. These results indicate that Kruppel-like factor 5 accelerates mitotic entry in H-Ras-transformed cells by transcriptionally activating cyclin B1 and Cdc2, which leads to an increase in cyclin B1/Cdc2 kinase activity. PMID: 16102754
  26. These experiments show that cyclinB1 is able to maintain a bipolar spindle even after sister chromatids had become separated and suggest an important role of hKid in this process. PMID: 16724106
  27. The present study was aimed to investigate the possibility that selenium (Se)-induced oxidative stress mediated alterations in Cdc25c and p21 may cause modulations in the CDC2/Cyclin B1 complex responsible for G2/M phase checkpoint in spermatogenesis. PMID: 17160696
  28. DEDD acts as a novel inhibitor of the mitotic Cdk1/cyclin B1 complex and is an impeder of cell mitosis, and its absence critically influences cell and body size via modulation of rRNA synthesis PMID: 17283331
  29. Selenium-induced oxidative-stress-mediated modulations of thia cell cycle regulators may cause DNA damage. PMID: 17320365
  30. cyclin B1 may have a role in esophageal squamous cell carcinoma invasive growth and metastasis PMID: 18048386
  31. Data show that in mouse oocytes, excess securin causes stabilization of cyclin B and precocious entry into M-phase. PMID: 18364698
  32. the suppression of nuclear import of cyclin B1, the induction of Wee1 kinase activity, and the transient nuclear accumulation of p21(CIP1/WAF1) may play important roles in the transient cell cycle delay in response to ionizing radiation PMID: 18425394
  33. REVIEW. Some cyclins exhibit unique patterns of expression in germ cells that suggest possible concomitant distinct functions; embryonic lethality has obviated understanding of the germline functions of cyclin A2 and cyclin B1 PMID: 19001847
  34. cyclin B1 has a role in inducing anticarcinogenic T-cell and antibody responses PMID: 19666607
  35. cyclin B1 vaccination delays spontaneous cyclin B1(+) tumor growth and increases median survival of tumor-bearing p53 deficient mice. PMID: 19769738
  36. PKA regulates cell cycle progression of fertilized eggs by interfering with the degradation of cyclin B. PMID: 15580572

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Subcellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families Cyclin family, Cyclin AB subfamily
Database Links

KEGG: mmu:268697

STRING: 10090.ENSMUSP00000071989

UniGene: Mm.260114

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