Recombinant Mouse GDNF family receptor alpha-like (Gfral), partial (Active)

GFRAL serves as the exclusive receptor for GDF15, a stress-response cytokine implicated in metabolic regulation and body weight homeostasis, making precise characterization of this interaction essential for metabolic drug discovery. This recombinant mouse GFRAL fragment (residues 20–349) encompasses the extracellular ligand-binding domain and is expressed in mammalian cells to preserve the native glycosylation and disulfide bonding critical for conformational integrity. Functional ELISA confirms robust ligand engagement, with immobilized GFRAL (5 µg/mL) binding mouse GDF15 at an EC50 of 7.926–10.52 ng/mL, providing a suitable basis for receptor-ligand interaction assays, competitive inhibition studies, blocking antibody screening, and small-molecule inhibitor discovery campaigns. Purity exceeds 95% by SDS-PAGE and endotoxin levels remain below 1.0 EU/µg, which satisfies the quality thresholds commonly required for SPR- or BLI-based affinity characterization, epitope mapping, and use as a positive control in quantitative binding assays.