GFRAL

The Glial cell line-derived neurotrophic factor family receptor alpha-like protein (GFRAL) is the specific receptor for growth differentiation factor 15 (GDF15). It forms a functional complex with RET tyrosine kinase, activating downstream RAS-MAPK and PI3K-AKT pathways upon ligand binding. This axis regulates energy homeostasis by suppressing appetite, reducing food intake, and enhancing energy expenditure, serving as a key metabolic modulator.
Dysregulation of the GDF15-GFRAL pathway is associated with obesity and cachexia. GFRAL agonists are in early clinical development for obesity, leveraging their anorexigenic effects. For cancer-related cachexia, elevated GDF15 drives weight loss; thus, GFRAL antagonists are being investigated to block this signaling, improving patient appetite and body mass.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

GFRAL Antibody (CSB-PA751020LA01HU)

Validated Data

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), A549 whole cell lysate(20µg), 293 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)Mouse Brain tissue lysate(20µg)
All lanes: GFRAL antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 30s

Immunofluorescence staining of Hela cell with CSB-PA751020LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The following GFRAL reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

GFRAL Antibodies

GFRAL Proteins

GFRAL ELISA Kits

GFRAL Antibodies

GFRAL Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA751020LA01HU	GFRAL Antibody	Human,mouse	WB, IF
CSB-PA751020LB01HU	GFRAL Antibody, HRP conjugated	Human	ELISA
CSB-PA751020LC01HU	GFRAL Antibody, FITC conjugated	Human
CSB-PA751020LD01HU	GFRAL Antibody, Biotin conjugated	Human	ELISA

GFRAL Proteins

GFRAL Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP750964MO1 CSB-EP750964MO1 CSB-BP750964MO1 CSB-EP750964MO1-B	Recombinant Mouse GDNF family receptor alpha-like (Gfral), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-MP750964MO1	Recombinant Mouse GDNF family receptor alpha-like (Gfral), partial (Active)	Mammalian cell

GFRAL Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP751020HU CSB-BP751020HU CSB-MP751020HU CSB-EP751020HU-B	Recombinant Human GDNF family receptor alpha-like (GFRAL), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP751020HU	Recombinant Human GDNF family receptor alpha-like (GFRAL), partial	E.coli
CSB-EP751020HUb1	Recombinant Human GDNF family receptor alpha-like (GFRAL), partial	E.coli

GFRAL ELISA Kit

GFRAL ELISA Kit for Mus musculus (Mouse)

Code	Product Name	Sample Types	Sensitivity
CSB-EL009383MO	Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit	serum, plasma, tissue homogenates, cell lysates	6.25 pg/mL

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GFRAL

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