Recombinant Mouse GDNF family receptor alpha-like (Gfral), partial

Code CSB-YP750964MO1
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP750964MO1
MSDS
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Source E.coli
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Code CSB-EP750964MO1-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750964MO1
MSDS
Size Pls inquire
Source Baculovirus
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gfral
Uniprot No.
Alternative Names
Gfral; Gral; GDNF family receptor alpha-like
Species
Mus musculus (Mouse)
Expression Region
20-349
Target Protein Sequence
Q TNDCAHLIQK CLIDANGCEQ SWRSMEDTCL TPGDSCKINN SLHCNLSIQA LVEKNFQFKE CLCMDDLHCT VNKLFGKKCT NKTDNMEKDN KDKWNLTTTP FYHGFKQMQS CLEVTEACVG DVVCNAQLAL YLKACSANGN LCDVKHCQAA IRFFYQNMPF NTAQMLAFCD CAQSDIPCQQ SKETLHSKPC ALNIVPPPTC LSVIHTCRND ELCRTHYRTF QTECWPHITG KCHEDETCIS MLGKQDLTCS GSESCRAAFL GTFGTVLQVP CACRGVTQAE EHVCMIFQHM LHSKSCFNYP TPNVKDISSY EKKNSKEITL TGFNSFFNG
Protein Length
Extracellular domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
Gene References into Functions
  1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
Subcellular Location
Cell membrane; Single-pass membrane protein; Extracellular side.
Protein Families
GDNFR family
Tissue Specificity
Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
Database Links

KEGG: mmu:404194

UniGene: Mm.213862

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