Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Instructions
Code CSB-EL009383MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name GDNF family receptor alpha like
Alternative Names Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
Abbreviation GFRAL
Uniprot No. Q6SJE0
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-98
1:2 Average % 101
Range % 97-107
1:4 Average % 94
Range % 90-98
1:8 Average % 97
Range % 92-101
Recovery
The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.859 1.952 1.906 1.766
800 1.258 1.260 1.259 1.119
400 0.720 0.734 0.727 0.587
200 0.466 0.489 0.478 0.338
100 0.315 0.328 0.322 0.182
50 0.229 0.235 0.232 0.092
25 0.172 0.176 0.174 0.034
0 0.138 0.141 0.140  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Gene References into Functions
  1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
Subcellular Location Membrane, Single-pass membrane protein
Protein Families GDNFR family
Tissue Specificity Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
Database Links

KEGG: mmu:404194

UniGene: Mm.213862

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