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Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

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Code	CSB-EL009383MO
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Product Details

Target Name

GDNF family receptor alpha like

Alternative Names

Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit

Abbreviation

GFRAL

Uniprot No.

Q6SJE0

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	101
	Range %	97-107
1:4	Average %	94
	Range %	90-98
1:8	Average %	97
	Range %	92-101

Recovery

The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	88	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	1.859	1.952	1.906	1.766
800	1.258	1.260	1.259	1.119
400	0.720	0.734	0.727	0.587
200	0.466	0.489	0.478	0.338
100	0.315	0.328	0.322	0.182
50	0.229	0.235	0.232	0.092
25	0.172	0.176	0.174	0.034
0	0.138	0.141	0.140

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

Citations

Hippocampal and Prefrontal Cortical Brain Tissue Levels of Irisin and GDF15 Receptor Subunits in Children TC Jackson,Nutrition & Metabolism,2021

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Target Data

Gene References into Functions	isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
Subcellular Location	Membrane, Single-pass membrane protein
Protein Families	GDNFR family
Tissue Specificity	Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
Database Links	KEGG: mmu:404194 UniGene: Mm.213862

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