Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Code CSB-EL009383MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
GDNF family receptor alpha like
Alternative Names
Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
Abbreviation
GFRAL
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-98
1:2 Average % 101
Range % 97-107
1:4 Average % 94
Range % 90-98
1:8 Average % 97
Range % 92-101
Recovery
The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.859 1.952 1.906 1.766
800 1.258 1.260 1.259 1.119
400 0.720 0.734 0.727 0.587
200 0.466 0.489 0.478 0.338
100 0.315 0.328 0.322 0.182
50 0.229 0.235 0.232 0.092
25 0.172 0.176 0.174 0.034
0 0.138 0.141 0.140  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse GFRAL ELISA Kit was designed for the quantitative measurement of Mouse GFRAL protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
Gene References into Functions
  1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
Subcellular Location
Cell membrane; Single-pass membrane protein; Extracellular side.
Protein Families
GDNFR family
Tissue Specificity
Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
Database Links

KEGG: mmu:404194

UniGene: Mm.213862

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