Recombinant Mouse Guanine nucleotide-binding protein G(o) subunit alpha(Gnao1)

Code CSB-YP009593MOb0
Size US$2474
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Gnao1
Uniprot No. P18872
Research Area others
Alternative Names Gnao1; Gna0; GnaoGuanine nucleotide-binding protein G(o) subunit alpha
Species Mus musculus (Mouse)
Source Yeast
Expression Region 2-354aa
Target Protein Sequence GCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKTDSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAGDYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 42.5kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.
Gene References into Functions
  1. Galphao +/- mice developed tolerance faster and displayed more severe naltrexone-precipitated withdrawal in some behaviors than did wild-type PMID: 28971229
  2. Go signaling is essential for the structural and functional integrity of the main olfactory epithelium and for specification of olfactory bulb interneurons. PMID: 27625425
  3. Data obtained support the hypothesis that GalphaO-mediated signaling within the presympathetic circuits of the rostral ventral lateral medulla contributes to the autonomic control of the heart. PMID: 27528004
  4. Necdin is a candidate downstream effector for Galphao. PMID: 25012566
  5. This heterozygous Gnao1 +/G184S mutation produces early neonatal and adult lethality accompanied by epileptiform discharges. PMID: 24700286
  6. These results highlight that sensory neurons of the Galphao-expressing vomeronasal subsystem, together with the receptors they express and the molecular cues they detect, control a wide range of fundamental mating and reproductive behaviors in female mice. PMID: 24886577
  7. The Goalpha expressing vomeronasal receptor cells led to all areas of the accessory olfactory bulb (AOB). PMID: 22383629
  8. Data indicate that rectifying potassium channel Kir2.4 interacts with G-protein subunit Galphao. PMID: 23339194
  9. Data show that Galphao is an essential requirement for the display of male-male territorial aggression as well as maternal aggression. PMID: 21768373
  10. Data suggest that the novel R234H mutation imparts oncogenic properties to Galphao by accelerating nucleotide exchange and rendering it constitutively active. PMID: 21317923
  11. The 2.9 A crystal structure of the enigmatic, neuronal G protein Galpha(o) in the GTP hydrolytic transition state, complexed with RGS16. PMID: 18434540
  12. Go alpha subunit mainly appears to be bound to the cell membrane and concentrated in the thin finger-like dendrites of rod bipolar cells, as well as in green fluorescent protein-positive cone bipolar cells. PMID: 12454992
  13. Binding of activated Galphao to GRIN1 induces activation of Cdc42, which leads to morphological changes in neuronal cells. PMID: 15585744
  14. our findings establish an essential role for G(o) in the antiadrenergic effect of muscarinic agent on hyperpolarization current PMID: 18156202
  15. Go2alpha optimizes vesicular filling which is instrumental for normal dopamine functioning and for the development of drug-induced behavioral sensitization. PMID: 18606864
  16. Data show that Galphao colocalized with GRIN1 at neuronal dendrites and axons, and Galphao-GRIN1 pathway could mediate roles in neuronal migration and differentiation at embryonic stages. PMID: 18729205
  17. Report role of Galpha0 in the control of in vivo heart rate dynamics. PMID: 18832081
  18. Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity. PMID: 19225179
  19. The G alpha(o) protein in the locus coeruleus as contributing to interindividual variability in physical dependence on opioids in mice. PMID: 19460419
  20. Data indicate that MC4R mutant is able to selectively interact with G(i/o) proteins. PMID: 19648111

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Subcellular Location Cell membrane, Membrane, Lipid-anchor
Protein Families G-alpha family, G(i/o/t/z) subfamily
Database Links

KEGG: mmu:14681

STRING: 10090.ENSMUSP00000034198

UniGene: Mm.251445

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