Product Details

Purity

>85% (SDS-PAGE)

Target Names

Hhip

Uniprot No.

Q7TN16

Alternative Names

Hhip; HipHedgehog-interacting protein; HHIP; HIP

Species

Mus musculus (Mouse)

Expression Region

18-700

Target Protein Sequence

FFE GDAKFGERNE GSGARRRRCL NGNPPKRLKR RDRRVMSQLE LLSGGEILCG GFYPRVSCCL QSDSPGLGRL ENKIFSATNN SECSRLLEEI QCAPCSPHSQ SLFYTPERDV LDGDLALPLL CKDYCKEFFY TCRGHIPGLL QTTADEFCFY YARKDAGLCF PDFPRKQVRG PASNYLGQME DYEKVGGISR KHKHNCLCVQ EVMSGLRQPV SAVHSGDGSH RLFILEKEGY VKILTPEGEL FKEPYLDIHK LVQSGIKGGD ERGLLSLAFH PNYKKNGKLY VSYTTNQERW AIGPHDHILR VVEYTVSRKN PHQVDVRTAR VFLEVAELHR KHLGGQLLFG PDGFLYIILG DGMITLDDME EMDGLSDFTG SVLRLDVDTD MCNVPYSIPR SNPHFNSTNQ PPEVFAHGLH DPGRCAVDRH PTDININLTI LCSDSNGKNR SSARILQIIK GRDYESEPSL LEFKPFSNGP LVGGFVYRGC QSERLYGSYV FGDRNGNFLT LQQSPVTKQW QEKPLCLGAS SSCRGYFSGH ILGFGEDELG EVYILSSSKS MTQTHNGKLY KIVDPKRPLM PEECRVTVQP AQPLTSDCSR LCRNGYYTPT GKCCCSPGWE GDFCRIAKCE PACRHGGVCV RPNKCLCKKG YLGPQCEQVD RNVRRVTRAG ILDQIIDMTS YLLDLTSYIV

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Modulates hedgehog signaling in several cell types, including brain and lung through direct interaction with members of the hedgehog family. Soluble forms inhibit Shh-induced differentiation in the fibroblast cell line C3H/10T1/2.

Gene References into Functions

To explore the molecular pathways which contribute to increased susceptibility, we performed metabolomic profiling using high performance liquid chromatography tandem mass spectroscopy (LC/MS-MS) on plasma, urine, and lung tissue of Hhip (+/-) heterozygotes and wild type (Hhip (+/+)) C57/BL6 mice exposed to either room-air or cigarette smoke for six months. PMID: 28566717
reduced lung function and age-related spontaneous emphysema development in Hhip(+/-) mice may be caused by increased oxidative stress levels in murine lungs as a result of haploinsufficiency of Hhip. PMID: 27444019
the heterozygous knockdown of hhip in mice could affect Hh signaling in tumor fibroblasts, which could cause the increased production of the growth factor VEGF. This signaling, via a paracrine effect on endothelial cells, increased tumor vascular density. PMID: 28127049
loss of AT2 R is associated with podocyte loss/dysfunction and is mediated, at least in part, via augmented ectopic hedgehog interacting protein expression in podocytes PMID: 28722118
PTCH1, PTCH2 and HHIP1 all contribute to ligand-dependent antagonism of sonic hedgehog signaling. PMID: 23900540
Estradiol improves the recovery of injured nerves by downregulating the Shh inhibitor hedgehog-interacting protein and increasing Shh-induced angiogenesis. PMID: 22330336
These results suggest that reduced expression of HIP, a naturally occurring Hh pathway antagonist, in tumor neo-vasculature may contribute to increased Hh signaling within the tumor and possibly promote angiogenesis. PMID: 15294024
hedgehog antagonists patched 1 and Hhip1 have roels in growth and pattern of the mammalian neural tube PMID: 15576403
hypothesis for a functional link between nitric oxide and Shh signaling and for a nonredundant role of Hip and Ptc1 in the adult brain PMID: 16272887
These data indicate that Hh signaling plays a role in adult gut epithelial homeostasis by regulating epithelial cell migration from crypt to villus tip and by enhancing apoptosis. PMID: 16439469
These results strongly suggest that GSK3beta phosphorylates Sufu to positively regulate Hedgehog signaling in mammalian cells. PMID: 17182001

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Subcellular Location

Cell membrane; Peripheral membrane protein. Secreted. Note=The last 22 C-terminal amino acids may participate in cell membrane attachment.

Protein Families

HHIP family

Tissue Specificity

In the adult brain, high expression found in the ventral cochlear nucleus, medial habenula, indusium griseum and tenia tecta. Some expression also in the caudate putamen, the nucleus accumbens, the ventral pallidum and in the superficial layers of the sup

Database Links

KEGG: mmu:15245

STRING: 10090.ENSMUSP00000078047

UniGene: Mm.254493

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Recombinant Mouse Hedgehog-interacting protein (Hhip)

Product Details

Customer Reviews and Q&A

Target Background

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