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Recombinant Mouse Heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), partial

Recombinant Mouse Heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), partial

Code	CSB-YP010746MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP010746MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP010746MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP010746MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP010746MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Hs2st1

Uniprot No.

Q8R3H7

Alternative Names

Hs2st1; Hs2stHeparan sulfate 2-O-sulfotransferase 1; 2-O-sulfotransferase; 2-OST; 2OST; EC 2.8.2.-

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Required for metanephric development of kidney formation, suggesting that 2-O-sulfation within HS is essential for signaling between ureteric bud and metanephric mesenchyme. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues.

Gene References into Functions

Inactivation of uronyl 2-O-sulfotransferase in endothelial cells (Hs2st), an enzyme that acts downstream from Ndst1, results in enhanced neutrophil recruitment in several models of acute inflammation. PMID: 22791291
Plasma lipoprotein clearance depends on specific subclasses of sulfate groups and not on overall charge of the chains. PMID: 19889634
The phenotype of Hs2st(-/-) mutant kidneys suggests that signalling between two tissues, ureteric bud and metanephric mesenchyme, is disrupted. PMID: 12975615
mice homozygous for a null mutation in Hs2st indicated reduction in the thickness of the embryonic cerebral cortex; critical role for 2-O-sulphation of heparan sulphate proteoglycan in regulating cell proliferation during cerebral cortex development PMID: 14654220
Hs2st and/or Hs6st1 expression coincides with Slit expression domains at locations where retinal ganglion cell(RGC) axons make navigation errors in Hs2st-/- and Hs6st1-/- mutants, and Hs6st1-/-RGC axons are less sensitive to Slit2 repulsion. PMID: 16807321

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Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Sulfotransferase 3 family

Tissue Specificity

Widely expressed. Expressed at higher level in lung and brain. Weakly expressed in spleen.

Database Links

KEGG: mmu:23908

STRING: 10090.ENSMUSP00000043066

UniGene: Mm.12863

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