Recombinant Mouse Homeobox expressed in ES cells 1 (Hesx1)

Code CSB-YP717221MO
MSDS
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Source Yeast
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Code CSB-EP717221MO
MSDS
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Source E.coli
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Code CSB-EP717221MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717221MO
MSDS
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Source Baculovirus
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Code CSB-MP717221MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Hesx1; Hes-1; Rpx; Homeobox expressed in ES cells 1; Anterior-restricted homeobox protein; Homeobox protein ANF; Rathke pouch homeo box
Species
Mus musculus (Mouse)
Expression Region
1-185
Target Protein Sequence
MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG DSEKGGNPPL HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK RELSWYRGRR PRTAFTQNQV EVLENVFRVN CYPGIDIRED LAQKLNLEED RIQIWFQNRR AKMKRSRRES QFLMAKKPFN PDLLK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.
Gene References into Functions
  1. Hesx1 plays a significant role in sustaining pluripotency in embryonic stem cells.Hesx1 transcriptionally suppresses differentiation-related genes. PMID: 26188092
  2. Wnt inhibition during the first day has larger impact on the activation of Hesx1 and Six3, suggesting that in embryos Wnt inhibition caused by Dkk1 contributes greatly in the establishment of the anterior forebrain precursor. PMID: 24520934
  3. Variability in Hes1 expression therefore helps to explain why STAT3 responsiveness varies between individual ES cells, and this in turn helps to explain why pluripotent cells commit to differentiate asynchronously.. PMID: 23649667
  4. Studies suggest that TLE1 and TLE3 might also play roles independent of HESX1 by interacting with other transcription factors like PROP1. PMID: 20181723
  5. Data suggest that formation of a heterodimer between HESX1 and PROP1 allows HESX1 to become active, and that PROP1 then replaces HESX1 to advance to the middle stage of pituitary development. PMID: 19879326
  6. A novel inducible element, activated by contact with Rathke's pouch, is present in the regulatory region of the Hesx1 homeobox gene. PMID: 12885556
  7. the domains of Hes-1 needed to block adipogenesis coincide with those necessary for transcriptional repression. PMID: 16282371
  8. Conserved regulatory elements establish the dynamic expression of Rpx in early development. PMID: 16527264
  9. The absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. PMID: 17360769
  10. Haploinsufficiency of both Six3 and Hesx1 leads to severe pituitary dysmorphogenesis during development, which results in hypopituitarism with dramatic postnatal growth retardation. PMID: 18775421
  11. Hesx1(I26T/I26T) embryos show pituitary defects comparable with Hesx1(-/-) mouse mutants, with frequent occurrence of ocular abnormalities, although the telencephalon develops normally PMID: 19093031

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Subcellular Location
Nucleus.
Protein Families
ANF homeobox family
Tissue Specificity
High levels found in the embryonic liver, lower level expression seen in the viscera, amnion and yolk sac.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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