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CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis-SUMO tag sequence into the targeted gene encoding the 22-159aa of the mouse Jchain. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant mouse Jchain protein carrying N-terminal 6xHis-SUMO tag. The SDS-PAGE assayed the purity of this recombinant Jchain protein greater than 90%. This Jchain protein migrated along the gel to a band of about 30 kDa molecular weight.
Immunoglobulin J chain is a protein encoding by a gene named Jchain in mouse and a gene named JCHAIN in human. This protein is a protein component of the antibodies IgM and IgA. The J chain-joined dimer is a nucleating unit for the IgM pentamer. And it induces dimers and/or larger polymers in the case of IgA. Moreover, it also helps to bind these immunoglobulins to secretory component. The diseases involved JCHAIN include Alpha Chain Disease and Climatic Droplet Keratopathy. Additionally, a study have reported that the upregulation of JCHAIN was likely related to tumor aggression, as studied in a cohort of patients with acute lymphoblastic leukemia (ALL).
|Purity||Greater than 90% as determined by SDS-PAGE.|
Jchain; IgjImmunoglobulin J chain
|Species||Mus musculus (Mouse)|
|Target Protein Sequence||GDDEATILADNKCMCTRVTSRIIPSTEDPNEDIVERNIRIVVPLNNRENISDPTSPLRRNFVYHLSDVCKKCDPVEVELEDQVVTATQSNICNEDDGVPETCYMYDRNKCYTTMVPLRYHGETKMVQAALTPDSCYPD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Protein Length||Full Length of Mature Protein|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Tris-based buffer,50% glycerol|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
|Gene References into Functions||