Recombinant Mouse Interferon-induced guanylate-binding protein 2 (Gbp2)

Code CSB-YP897446MO
MSDS
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Source Yeast
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Code CSB-EP897446MO
MSDS
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Source E.coli
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Code CSB-EP897446MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897446MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP897446MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gbp2
Uniprot No.
Alternative Names
Gbp2; Guanylate-binding protein 2; EC 3.6.5.-; GTP-binding protein 2; GBP-2; mGBP-2; mGBP2; Guanine nucleotide-binding protein 2; Interferon-induced guanylate-binding protein 2
Species
Mus musculus (Mouse)
Expression Region
1-586
Target Protein Sequence
MASEIHMSEP MCLIENTEAQ LVINQEALRI LSAITQPVVV VAIVGLYRTG KSYLMNKLAG KRTGFSLGST VQSHTKGIWM WCVPHPKKAG QTLVLLDTEG LEDVEKGDNQ NDCWIFALAV LLSSTFIYNS IGTINQQAMD QLHYVTELTD LIKSKSSPDQ SGVDDSANFV GFFPTFVWTL RDFSLELEVN GKPVTSDEYL EHSLTLKKGA DKKTKSFNEP RLCIRKFFPK RKCFIFDRPA QRKQLSKLET LREEELCGEF VEQVAEFTSY ILSYSSVKTL CGGIIVNGPR LKSLVQTYVG AISNGSLPCM ESAVLTLAQI ENSAAVQKAI THYEEQMNQK IQMPTETLQE LLDLHRPIES EAIEVFLKNS FKDVDQKFQT ELGNLLVAKR DAFIKKNMDV SSARCSDLLE DIFGPLEEEV KLGTFSKPGG YYLFLQMRQE LEKKYNQAPG KGLQAEAMLK NYFDSKADVV ETLLQTDQSL TEAAKEVEEE RTKAEAAEAA NRELEKKQKE FELMMQQKEK SYQEHVKKLT EKMKDEQKQL LAEQENIIAA KLREQEKFLK EGFENESKKL IREIDTLKQN KSSGKC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.
Gene References into Functions
  1. GBP2 can effectively regulate beta-glucan-induced maturation of DCs, thus suppressing the proliferation of T cells. PMID: 29089068
  2. This study demonstrated that Gbeta2 is important for neuronal migration, especially in the multipolar-bipolar transition. PMID: 28334111
  3. this study identified guanylate-binding proteins GBP2 and GBP5 as key activators of AIM2 during infection with F. novicida. PMID: 25774716
  4. This study begins to define the properties of mGBP-2 responsible for inhibiting cell spreading. PMID: 20950129
  5. Murine guanylate binding protein 2 (mGBP2) controls Toxoplasma gondii replication. PMID: 23248289
  6. the inducible multimerization of mGBP2 is dependent on a functional GTPase domain. PMID: 22730319
  7. IRGM proteins indirectly modulate the localization of GBP2 through a distinct mechanism from that through which they regulate IRG protein localization PMID: 21757726
  8. mGBP-2 inhibits TNF-alpha activation of endogenous Rac1 and constitutively activate Rac can restore NF-kappaB transcription in the presence of mGBP-2. This is a novel mechanism by which IFNs can inhibit the cytokine induction of MMP-9 expression. PMID: 21502320
  9. Both IFN-gamma and mGBP-2 also inhibit cell spreading initiated by platelet-derived growth factor treatment, which is also accompanied by inhibition of Rac activation by mGBP-2. PMID: 20505078
  10. inhibits replication of both vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV); a wild type GTP binding motif was not required for VSV inhibition but was required for inhibition of EMCV PMID: 15717119
  11. mGBP-2, confers resistance to paclitaxel-induced cytotoxicity without inhibiting multinucleation. PMID: 16914101
  12. The data support the assumption that the main role of STAT1 in activating gbp2 transcription is to provide transcriptionally competent chromatin, whereas the function of IRF1 may lie in directly contacting RNA polymerase II complexes. PMID: 17293456

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Subcellular Location
Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus membrane. Membrane; Lipid-anchor.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3-type GTPase family, GB1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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