Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Il31ra

Uniprot No.

Q8K5B1

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Il31ra; GlmrInterleukin-31 receptor subunit alpha; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL-31R-alpha; IL-31RA; GLM-R; mGLM-R; Gp130-like monocyte receptor; Gp130-like receptor; Novel cytokine receptor 10; NR10; ZcytoR17

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

19-499aa

Target Protein Sequence

VLPTKPENISCVFYFDRNLTCTWRPEKETNDTSYIVTLTYSYGKSNYSDNATEASYSFPRSCAMPPDICSVEVQAQNGDGKVKSDITYWHLISIAKTEPPIILSVNPICNRMFQIQWKPREKTRGFPLVCMLRFRTVNSSHWTEVNFENCKQVCNLTGLQAFTEYVLALRFRFNDSRYWSKWSKEETRVTMEEVPHVLDLWRILEPADMNGDRKVRLLWKKARGAPVLEKTFGYHIQYFAENSTNLTEINNITTQQYELLLMSQAHSVSVTSFNSLGKSQEAILRIPDVHEKTFQYIKSMKAYIAEPLLVVNWQSSIPAVDTWIVEWLPEAAMSKFPALSWESVSQVTNWTIEQDKLKPFTCYNISVYPVLGHRVGEPYSIQAYAKEGTPLKGPETRVENIGLRTATITWKEIPKSARNGFINNYTVFYQAEGGKELSKTVNSHALQCDLESLTRRTSYTVWVMASTRAGGTNGVRINFKT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

59.2 kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this recombinant Mouse Il31ra protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the Il31ra protein. N-terminal 6xHis tag was used in the process. The purity is 0.85 determined by SDS-PAGE.

The receptor IL-31RA is the receptor of IL-31, which has been found unregulated in skin and lung fibroblasts from Scleroderma. IL-31 acts via a heterodimeric receptor comprising IL-31RA and oncostatin M, expressed by multiple cell types, and signalling via PI3K, ERK and JAK/STAT pathways. Interleukin (IL)-31 is a cytokine produced mainly by Th2 cells. And it signals via the IL-31RA and oncostatin M receptor β heterodimer. IL-31RA is expressed in both human skin and dorsal root ganglions. IL-31RA is a critical neuroimmune link between Th2 cells and the sensory nerves. Some resports suggested that that IL-31RA is expressed not only in nerve fibres but also in keratinocytes in the dermis from Alzheimer patients. Besides, researchers observed that administration of IL-31 upregulates IL-31RA in dorsal root ganglia and causes severe itch-associated scratching behaviour in mice.

Target Background

Function

Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Mediates IL31-induced itch, probably in a manner dependent on cation channels TRPA1 and TRPV1. Positively regulates numbers and cycling status of immature subsets of myeloid progenitor cells in bone marrow in vivo and enhances myeloid progenitor cell survival in vitro.

Gene References into Functions

Anti-IL-31 receptor antibody is shown to be a potential therapeutic option for treating itch and dermatitis in mice PMID: 24946165
IL31RA is a functional receptor expressed by a small subpopulation of IL-31RA(+)/TRPV1(+)/TRPA1(+) neurons and is a critical neuroimmune link between TH2 cells and sensory nerves for the generation of T cell-mediated itch. PMID: 24373353
Gp130(757F/F) mice rapidly develop distal stomach tumors, with loss of SHP2/Erk/AP-1 transcriptional regulation exemplified by decreased TFF1 expression and increased STAT1/3 regulated genes such as Reg I. PMID: 14699500
IL-31 receptor alpha signaling is a novel negative regulatory pathway that specifically limits T helper cell type 2 inflammation. PMID: 17353366

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, presynaptic cell membrane. Cell projection, axon.

Protein Families

Type I cytokine receptor family, Type 2 subfamily

Tissue Specificity

Expressed in a subset of dorsal root ganglia neurons. Expressed in spinal cord and trigeminal ganglion (at protein level). Expressed in skin, testis, bone marrow and thymus.

Database Links

KEGG: mmu:218624

STRING: 10090.ENSMUSP00000058045

UniGene: Mm.380801

Code	CSB-EP818362MO
Abbreviation	Recombinant Mouse Il31ra protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP818362MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Il31ra. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP818362MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Il31ra.
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Recombinant Mouse Interleukin-31 receptor subunit alpha (Il31ra), partial

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