Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Lingo1

Uniprot No.

Q9D1T0

Research Area

Others

Alternative Names

Lingo1; Lern1; Lrrn6a; Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

37-555aa

Target Protein Sequence

PPRCECSAQDRAVLCHRKRFVAVPEGIPTETRLLDLGKNRIKTLNQDEFASFPHLEELELNENIVSAVEPGAFNNLFNLRTLGLRSNRLKLIPLGVFTGLSNLTKLDISENKIVILLDYMFQDLYNLKSLEVGDNDLVYISHRAFSGLNSLEQLTLEKCNLTSIPTEALSHLHGLIVLRLRHLNINAIRDYSFKRLYRLKVLEISHWPYLDTMTPNCLYGLNLTSLSITHCNLTAVPYLAVRHLVYLRFLNLSYNPIGTIEGSMLHELLRLQEIQLVGGQLAVVEPYAFRGLNYLRVLNVSGNQLTTLEESAFHSVGNLETLILDSNPLACDCRLLWVFRRRWRLNFNRQQPTCATPEFVQGKEFKDFPDVLLPNYFTCRRAHIRDRKAQQVFVDEGHTVQFVCRADGDPPPAILWLSPRKHLVSAKSNGRLTVFPDGTLEVRYAQVQDNGTYLCIAANAGGNDSMPAHLHVRSYSPDWPHQPNKTFAFISNQPGEGEANSTRATVPFPFDIKTLIIAT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

62.9kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Mouse Lingo1 covers amino acids 37-555. This Lingo1 protein is expected to have a theoretical molecular weight of 62.9 kDa. This protein is generated in a e.coli-based system. The N-terminal 6xHis tag was fused into the coding gene segment of Lingo1, making it easier to detect and purify the Lingo1 recombinant protein in the later stages of expression and purification.

The mouse leucine-rich repeat and immunoglobulin-like domain-containing Nogo receptor-interacting protein 1 (Lingo1) is a transmembrane protein involved in regulating neuronal function. Lingo1 interacts with the Nogo receptor and plays a role in modulating myelination, neurite outgrowth, and synaptic plasticity. It is predominantly expressed in the nervous system, influencing neural development and regeneration. Research on Lingo1 encompasses studies on its involvement in neurodegenerative diseases, such as multiple sclerosis and Alzheimer's disease, where dysregulation may contribute to pathological processes. Understanding the functions of Lingo1 provides insights into the molecular mechanisms governing neural plasticity and offers potential therapeutic avenues for neurological disorders.

Target Background

Function

Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.

Gene References into Functions

These data provide morphometric and functional evidence of enhancement of remyelination associated with antagonism of LINGO-1 PMID: 25681574
LINGO-1, a transmembrane signaling protein, inhibits oligodendrocyte differentiation and myelination through intercellular self-interactions PMID: 22514275
a novel role for LINGO-1 in neural stem cell differentiation to neurons PMID: 22235341
inhibitory agents of LINGO-1 activity can protect dopaminergic neurons against degeneration PMID: 17726113
Lingo1 antagonist promotes spinal cord remyelination and axonal integrity in MOG-induced experimental autoimmune encephalomyelitis. PMID: 17906634

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Highly specific expression in the central nervous system. Predominant expression in neocortex, amygdala, hippocampus, thalamus and entorhinal cortex, with lower levels in cerebellum and basal nuclei.

Database Links

KEGG: mmu:235402

STRING: 10090.ENSMUSP00000059050

UniGene: Mm.246605

Code	CSB-EP880476MO
Abbreviation	Recombinant Mouse Lingo1 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 (Lingo1), partial

Product Details

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