Recombinant Mouse Microtubule-associated protein 2(Map2),partial

Code CSB-EP013408MO
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Map2
Uniprot No. P20357
Research Area Others
Alternative Names Map2; Mtap2; Microtubule-associated protein 2; MAP-2
Species Mus musculus (Mouse)
Source E.coli
Expression Region 213-559aa
Target Protein Sequence GMEGEKLPPVPFAQTFGTNLEDRKQSTEPSIVMPSIGLSAEPPAPKEPKDWFIEMPTESKKDEWGLAAPISPGPLTPMREKDVLEDIPRWEGKQFDSPMPSPFHGGSFTLPLDTMKNERVSEGPRPFAPVFFQSDDKVSLQDPSALATSKESSKDEEPLKDKADKVADVSISEVTTLLGNVHSPVVEGYVGENISGEVKVTTDQEKKETSAPSVQEPTLTETEPQTKLDEKSTVSIEEAVAKKEESFKLRDDKTGVIQTSTEQSFSKEDQKGQEHTIDELKQDSFPISLEQAVTDAAMTSKTLGKVTSEPEAVSERREIQGLFEEKTADKNKLEGAGSATIAEVEMP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 54.0kDa
Protein Length Partial
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Gene References into Functions
  1. MAP2c levels in different brain regions of aging mice were positively correlated with neuropsin expression. PMID: 24965600
  2. Accumulation of intraneuronal beta-amyloid 42 peptides is associated with early changes in microtubule-associated protein 2 in neurites and synapses. PMID: 23372648
  3. results demonstrate the structural and functional determinant underlying the v-KIND-MAP2 interaction that controls dendrite arborization patterns PMID: 21385318
  4. Increased MAP2 expression in malignant melanoma cells significantly inhibits tumor cell growth and neoplasm invasiveness, and induces apoptosis and cell cycle arrest. PMID: 20100193
  5. Data show that inhibition of ZNF238 (RP58) expression in mouse GNPs decreases the expression of the neuronal differentiation markers MAP2 and NeuN and downregulates the expression of the cell cycle arrest protein p27, a regulator of GNP differentiation. PMID: 20103640
  6. Nitric oxide affects the phosphorylation state of microtubule-associated protein 2 in brain. PMID: 11829530
  7. PKA binding to MAP2 and MAP2 phosphorylation is essential for the selective development of contextual memory. PMID: 12763072
  8. Multiple brain regions exhibit potential for endogenous recovery and/or reorganization of MAP2 after injury of moderate traumatic severity. PMID: 14588114
  9. As young adults, S100B transgenic mice exhibit significantly greater MAP-2-immunoreactivity in the hippocampus, however in older adults, less MAP-2 staining is exhibited. PMID: 15306236
  10. JNK1 phosphorylation of MAP2 plays an important role in defining dendritic architecture in the brain. PMID: 16000625
  11. We also propose MAP2-deficient mice as an ideal system for identifying protein phosphatases essential for dendritic functions. PMID: 16257389
  12. MAP2 was increased in old females compared with both young and middle-age females with no significant difference in males, suggesting it may constitute a compensatory response to age-related functional decline in mice. PMID: 16330151
  13. TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites PMID: 16901895
  14. v-KIND mediates a signaling pathway that links Ras and MAP2 to control dendrite growth PMID: 17984326
  15. AKAP150 and Mtap2 put TREK channels at the center of a complex protein network that finely tunes channel trafficking, addressing, and regulation. PMID: 18716213

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Subcellular Location Cytoplasm, cytoskeleton, Cell projection, dendrite
Database Links

KEGG: mmu:17756

STRING: 10090.ENSMUSP00000109650

UniGene: Mm.256966

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