Recombinant Mouse Muscarinic acetylcholine receptor M5 (Chrm5), partial

Code CSB-YP856351MO1
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Source Yeast
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Code CSB-EP856351MO1
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Source E.coli
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Code CSB-EP856351MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856351MO1
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Source Baculovirus
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Code CSB-MP856351MO1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Chrm5
Uniprot No.
Alternative Names
Chrm5; Muscarinic acetylcholine receptor M5
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Gene References into Functions
  1. This study demonistrated that activation of M5 receptors in the striatum induces an inhibition in dopamine release. PMID: 24573284
  2. Mutant mice with a deletion in the third intracellular loop of the muscarinic M5 receptor gene exhibited increased drinking compared to wild-type, but feeding was not increased. PMID: 11900778
  3. Deletion attenuates morphine reinforcement and withdrawal but not morphine analgesia. PMID: 12154229
  4. M5 mAChRs modulate of central dopamine function and the tone of cerebral blood vessels. Review. PMID: 12675128
  5. acetylcholine, a potent dilator of most vascular beds, virtually lost the ability to dilate cerebral arteries and arterioles in M5 muscarinic receptor -/- mice, suggesting that endothelial M(5) receptors mediate this activity in wild-type mice--review PMID: 14607263
  6. Male M5R-/- mice exhibited a significantly reduced cerebral blood flow (CBF) in the cerebral cortex, hippocampus, basal ganglia, and thalamus. Cortical and hippocampal pyramidal neurons from M5R-/- mice showed neuronal atrophy. PMID: 16956767
  7. Results suggest that M1 and M5 muscarinic cholinergic receptors are not involved in anti-viral immunity. PMID: 17286988
  8. M2 and M5 muscarinic receptors but not M4 muscarinic receptor or D2 dopamine receptors are needed for male USV production during male-female interactions PMID: 18382674
  9. In the absence of VTA M(5) receptors, ventral tegmental area cholinergic agent inputs inhibit locomotion. PMID: 18849356

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM5 sub-subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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