Recombinant Mouse Myosin-6 (Myh6), partial

Code CSB-YP015299MO
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Source Yeast
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Code CSB-EP015299MO
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Source E.coli
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Code CSB-EP015299MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015299MO
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Source Baculovirus
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Code CSB-MP015299MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Myh6
Uniprot No.
Alternative Names
Myh6; Myhca; Myosin-6; Myosin heavy chain 6; Myosin heavy chain; cardiac muscle alpha isoform; MyHC-alpha
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Muscle contraction.
Gene References into Functions
  1. The stress-induced Brg1-G9a/GLP-Dnmt3 interactions and sequence of repressive chromatin assembly on Myh6 promoter illustrates a molecular mechanism by which the heart epigenetically responds to environmental signals. PMID: 26952936
  2. Taken together, these results highlight both the importance of using alphaMyHC-Cre mice as controls in conditional knockout studies as well as the need for a less cardiotoxic Cre driver for the field. PMID: 26141530
  3. Data indicate that the cardiac myosin heavy chain alpha isoform (alphaMHC(403)) transgenic model closely recapitulates human disease. PMID: 25318870
  4. mutant sarcomeres manifest irreversible cardiomyocyte defects that induce LVH. PMID: 25677315
  5. Transfecting human beta-MHC V606M into the mouse cardiac alpha-MHC gene caused hypertrophic cardiomyopathy without left ventricular hypertrophy, disarray of myofibers, and interstitial fibrosis. Cyclosporine or Arg453Cys or Arg719Trp worsened the phenotype. PMID: 24829265
  6. Results indicate the downstream targets of insulin, cyclin D1, BAD, alpha-MHC, and GATA-4, elucidate a molecular mechanism of insulin in promoting cell proliferation and differentiation. PMID: 24020834
  7. Transgenic mouse alpha- and beta-cardiac myosins containing the R403Q mutation show isoform-dependent transient kinetic differences. PMID: 23580644
  8. Results show that the binding force between selenium and cardiac myosin (CM), which consists of two heavy chains (MHC) and two pairs of light chains: MLC1 and MLC2, was 100 times stronger than that of daunorubicin (DNR) and CM. PMID: 22190261
  9. hypertrophied myocytes after TAC have alpha-MyHC only. These data challenge the current paradigm of the fetal hypertrophic gene program and identify a new subpopulation of smaller working ventricular myocytes with more myosin. PMID: 21778428
  10. the alpha-isoform of myosin heavy chain is the pathogenic autoantigen for CD4+ T cells in myocarditis PMID: 21436590
  11. Silencing of aMHC is associated at its promoter with decreased H3K4me3, but not decreased H3ac. PMID: 21526716
  12. TCDD represses the expression of the cardiac development-specific Nkx2.5 homeobox transcription factor, of cardiac troponin-T and of alpha- and beta-myosin heavy chains. PMID: 20130022
  13. These results suggest that the depressed molecular function in cardiac myosin may initiate the events that cause the heart to remodel and become pathologically dilated. PMID: 16983074
  14. the alphaMHC gene, in addition to encoding a major cardiac contractile protein, regulates cardiac growth and gene expression in response to stress and hormonal signaling through miR-208 PMID: 17379774
  15. residues situated within or close to the actin-binding interface of the myosin head influence actin binding and thereby modulate actin-activated ATPase activity PMID: 17575272
  16. Development of hypertrophic cardiomyopathy due to alpha-myosin heavy chain mutations may be accelerated by male hormones or the absence of female hormones. PMID: 18281382
  17. functional consequences of the mutation are fundamentally changed depending upon the context of the cardiac MHC isoform PMID: 18480046

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Subcellular Location
Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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