Recombinant Mouse Neurogenic locus notch homolog protein 3 (Notch3), partial

Code CSB-YP733800MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP733800MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP733800MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP733800MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP733800MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Notch3
Uniprot No.
Alternative Names
Notch3; Neurogenic locus notch homolog protein 3; Notch 3) [Cleaved into: Notch 3 extracellular truncation; Notch 3 intracellular domain]
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May play a role during CNS development.
Gene References into Functions
  1. endothelial expression of Notch3 limits tumour angiogenesis; tumour growth and angiogenesis are increased when Notch3 is silenced in the stroma PMID: 28719575
  2. Double immunostaining with differentiation markers revealed that NOTCH3 was expressed in some undifferentiated and differentiated spermatogonia in mouse testes. PMID: 29161703
  3. We find that across species, the atypical receptor NOTCH3 is differentially overexpressed; it is progressively up-regulated with disease development and promotes tumor cell survival via activation of PI3k-Akt PMID: 27791012
  4. overexpression demonstrates an antiproliferative effect on established metastatic medullary thyroid cancer liver tumors PMID: 29128180
  5. Notch3 mutation impairs recovery of cardiac function post-myocardial ischemia. PMID: 28131704
  6. Notch3 plays an important role in the maintenance of quiescent neural stem cells in the subependymal zone. PMID: 29101245
  7. Knock-in mice with the R169C mutation (Notch3(R170C/R170C)) exhibited similar reductions in arterial lumen, and both TgNotch3(R169C) and Notch3(R170C/R170C) mice showed increased cerebral artery expression of Notch3 target genes. PMID: 27821617
  8. Elevated levels of TIMP3 and vitronectin, acting downstream of Notch3(ECD) deposition, play a role in CADASIL, producing divergent influences on early CBF deficits and later white matter lesions. PMID: 26648042
  9. Mutant Notch3 accumulates in pericytes and causes progressive pericyte loss and BBB leakage in the cerebral cortex in CADASIL mouse model. PMID: 26312599
  10. Relative to air-exposed controls, ozone increased bronchoalveolar lavage fluid protein, a marker of lung permeability, in all genotypes, but significantly greater concentrations were found in Notch4-/- compared with wild-type and Notch3-/- mice. PMID: 25658374
  11. The mechanism of Oncostatin M on cardiac ischemia/reperfusion injury is partly mediated by the Notch3/Akt pathway. PMID: 26093524
  12. miR-7a regulates the differentiation of Muller glia through the suppression of Notch3 expression. PMID: 26122050
  13. Study showed Notch3 expressed in proliferating hippocampal precursor cells and down-regulates their activation and proliferation; adult neurogenesis in CADASIL transgenic mice was altered prior to vascular abnormalities due to deficits in Notch3 signaling PMID: 25555543
  14. NOTCH3, but not NOTCH2 protects vascular smooth muscle cell from apoptosis. PMID: 25957400
  15. Notch3 protein activation in glomeruli promotes the development of progressive renal disease. PMID: 25421557
  16. A viral cyclin-CDK6 complex was found to be a regulator of Notch3 in lymphoma cells. PMID: 25483078
  17. Loss of Notch3 is associated with impaired neuronal maturation. PMID: 25164209
  18. study demonstrates activity-dependent reduction of DLL4 expression and proteolytic cleavage of Notch3 in the hypothalamic-neurohypophysial system. PMID: 24943269
  19. Notch3 is important for survival of vascular smooth muscle cells, and reveal a critical role for Notch3 and VSMC in blood vessel integrity and blood-brain barrier function in the mammalian vasculature. PMID: 25477343
  20. Endogenous activation of Notch3 signaling selectively controls the pool of undifferentiated progenitors of upper airways available for differentiation. PMID: 25564622
  21. Notch3 activation is sufficient but not required for inducing human T-lineage specification. PMID: 25381438
  22. These data indicate that both mTORC1 and Notch3 signaling are involved in HPH and the downstream effects of mTORC1 activation in HPH are partially dependent on the activation of Notch3 signaling. PMID: 24825564
  23. Stroke outcome and memory impairments are worse in notch3 null mice; lack of the notch3 receptor affects white matter stroke susceptibility. PMID: 24723319
  24. cerebral autosomal dominant arteriopathy with subcortical infarcts and leucoencephalopathy can develop without impairment of NOTCH3 signaling PMID: 24425116
  25. High notch3 expression is associated with inflammatory breast cancer that frequently metastasized. PMID: 23928992
  26. Notch3 is expressed in a highly clonogenic and transiently quiescent luminal progenitor population that gives rise to a ductal lineage. PMID: 24100291
  27. Ccr2 deficient mice display increased Notch3 signaling and dysregulated Notch ligand expression. PMID: 23492191
  28. Identify Grip2 and Notch3 target regulating myogenic response of cerebral vessels. PMID: 23117660
  29. Notch-3 receptor activation drives inflammation and fibrosis following tubulointerstitial kidney injury. PMID: 22806125
  30. Notch-3 expression is significantly upregulated in renal cells from mice with a targeted disruption of one Y box (YB)-1 allele. PMID: 21372592
  31. Suppression of Notch3 disrupts quiescence and promotes expansion of self-renewing populations. PMID: 21912215
  32. Notch3 is differentially expressed by progenitor thymocytes, peaking at the DN3 progenitor stage. Notch1 & Notch3 carry out nonoverlapping functions during thymocyte differentiation. Notch3 is not essential at later stages. PMID: 21768299
  33. cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy is associated with hypomorphic Notch 3 function in vascular smooth muscle cells. PMID: 21555590
  34. mechanism of Notch activation PMID: 21536678
  35. Data indicate that the cooperation among Notch3, protein kinase C, and p65/NF-kappaB subunit modulates Foxp3 expression. PMID: 21508258
  36. Data show that Notch3 is necessary for the adaptive response of the renal vasculature to vasoactive systems. PMID: 21518968
  37. Dominant mutations in NOTCH3 cause cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). PMID: 20967782
  38. Data suggest that selective inhibition of Notch signalling mediated by Notch3 or Delta-like1 may offer a new strategy for the treatment of RA. PMID: 21235539
  39. Notch3 pathway might be distinct from Notch1 in muscle regeneration. PMID: 20960513
  40. Notch3 is important for the investment of mural cells and is a critical regulator of developmental and pathological blood vessel formation PMID: 20689064
  41. critical role for lysosomes in the degradation PMID: 19735738
  42. Data show that that the increased Notch3-IC degradation correlates with higher levels of c-Cbl tyrosine phosphorylation in Notch3-IC/pTalpha(-/-) double-mutant thymocytes. PMID: 19966856
  43. component in spermatogenesis and Sertoli cells in neonatal mice PMID: 11700858
  44. expression pattern analyzed in the pre- and postnatal mouse brain; data show expression is conserved between mouse and human and suggest that the mouse is a valid system for analysis of CADASIL PMID: 12126955
  45. Ectopic expression of Notch3 in airway epithelium potentially contributes to the multistep evolution of lung cancer through the inhibition of terminal differentiation. PMID: 12673204
  46. Notch3 is observed in the lower compartment of developing olfactory neuroepithelium until it gradually disappears, which suggests a distinct role for Notch3 in the neurogenesis of the peripheral olfactory system. PMID: 15076712
  47. Heterodimerization of mutant Notch3 with Fringe is associated with pathophysiology of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy PMID: 15857853
  48. results support the idea that other Notch genes functionally compensate for Notch3 during embryonic development PMID: 15882997
  49. activated Notch3 can function as an oncogene in the developing brain, and link the Notch pathway to human choroid plexus tumor pathogenesis PMID: 16186803
  50. Notch3 plays role in the development and transformation of the mammary gland. PMID: 16507912

Show More

Hide All

Subcellular Location
Cell membrane; Single-pass type I membrane protein.; [Notch 3 intracellular domain]: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
Protein Families
NOTCH family
Tissue Specificity
Proliferating neuroepithelium.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1