Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Ppt1

Uniprot No.

O88531

Research Area

Others

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

28-306aa

Target Protein Sequence

DPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

33.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

Gene References into Functions

Our results indicate that PPT1 deficiency causes alterations in the mitochondrial respiratory chain. PMID: 27722792
Proteomics analysis on isolated cilia revealed 660 proteins, which differed in their abundance levels between wild type and Ppt1 knock out. PMID: 28334871
In the novel Cln1(R151X) mouse model of INCL. PMID: 26648046
Parkinson-like motor/sensorimotor deficits in Cln1-/- mice are not mediated by dopamine deficiency. PMID: 26238334
Data show that Cln1 mutations disrupt the maturation of cathepsin D in lysosome contributing to neuropathology of infantile neuronal ceroid lipofuscinoses and suggest cathepsin D deficiency to be common link between infantile and congenital form. PMID: 26160911
The authors have generated a Cln1 R151X point mutation mouse model that recapitulates the molecular, neuropathological and behavioral phenotypes of neuronal ceroid lipofuscinoses. PMID: 25205113
Data (including data from knockout mice) suggest that deficiency of Ppt1 leads to accumulation of granular osmiophilic deposits in many cell types, especially in astrocytes. [review-like article] PMID: 25233404
Data suggest that mouse macrophages express PPT1; PPT1 appears to contribute to 2-arachidonylglycerol hydrolysis activity in peritoneal macrophages in culture. PMID: 24083319
The simultaneous loss of both Cln1 and Cln5 genes might enhance the typical pathological phenotypes of these mice by disrupting or downregulating shared or convergent pathogenic pathways. PMID: 23065637
These results uncover a previously unknown phenotype associated with PPT1 deficiency, that of altered thermoregulation, which is associated with impaired lipolysis and neurotransmitter release to brown adipose tissue during cold exposure. PMID: 23139814
The addition of the small molecule PPT1 mimetic can further increase that response. PMID: 22310926
Glutamate receptor 4 (GluR4) AMPA receptor hypofunction and NMDA receptor hyperfunction phenotype show increased sensitivity in Ppt1-deficient neurons. PMID: 21971706
Data show a progressive breakdown of axons and synapses in the brains of two different models of NCL: Ppt1(-/-) model of infantile NCL and Cln6(nclf) model of variant late-infantile NCL. PMID: 19640925
The polarized axonal targeting of PPT1 may well indicate a role for PPT1 in the exocytotic pathway of neurons. PMID: 12483688
was only detected in developing testis not ovary PMID: 15128596
The severely affected PPT1-/- mouse CNS exhibited reduced volume of both cortical and subcortical regions, but with sparing of the cerebellum PMID: 15193291
Autopsy of PPT1 mutant mice revealed a severe loss of brain mass and accumulation of autofluorescent granular osmiophilic deposits, both characteristic of Neuronal Ceroid Lipofuscinosis. PMID: 15649713
We conclude that from an early age, neurons deficient in PPT1 enzyme activity display intrinsically abnormal properties that could potentially explain key features of the clinical disease, such as myoclonus and seizures. PMID: 16242638
Palmitoyl-protein thioesterase-1 deficiency mediates the activation of the unfolded protein response and neuronal apoptosis in infantile neuronal ceroid lipofuscinosis. PMID: 16368712
Age-dependent increase in LPC levels in the PPT1-KO mouse brain positively correlates with elevated expression of the genes characteristically associated with phagocytes. PMID: 17341491
Neuron-specific changes for F(1)-complex in the Ppt1-deficient cells and give clues for a possible link between lipid metabolism and neurodegeneration in Infantile neuronal ceroid lipofuscinosis. PMID: 18245779
We analyzed locus specific gene expression and showed regional clustering of Cln1 and three major genes of this pathway, further supporting a close functional relationship between the corresponding gene products PMID: 18371231
Palmitoyl protein thioesterase-1 deficiency impairs synaptic vesicle recycling at nerve terminals in humans and mice PMID: 18704195
This study suggests that neuronal-glial interactions are the core pathology in the palmitoyl protein thioesterase 1 deficient cerebellum. PMID: 19416667

Hide All

Subcellular Location

Lysosome. Secreted.

Protein Families

Palmitoyl-protein thioesterase family

Tissue Specificity

Highest level in testis and kidney, lower in heart, brain and lung and lowest in skeletal muscle.

Database Links

KEGG: mmu:19063

STRING: 10090.ENSMUSP00000030412

UniGene: Mm.277719

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

CAPTCHA verification *

Code	CSB-MP018587MO
MSDS	MSDS
Size	$318
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage