Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nectin3

Uniprot No.

Q9JLB9

Alternative Names

Nectin3; Pvrl3Nectin-3; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD antigen CD113

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Gene References into Functions

Nectin-3 expressed in dentate gyrus neurons may modulate adult neurogenesis, dendritic spine plasticity and the cognitive effects of early-life stress. PMID: 28872640
-afadin-specific C-terminal inserts may be involved in its preference of binding to nectin-3 and raise the possibility that there are proteins other than nectins that more preferentially bind s-afadin than l-afadin. PMID: 25263091
owing to the loss of Nectin-3, hair cells contact each other inappropriately and form abnormal junctions, ultimately resulting in abnormal hair bundle orientation and morphology PMID: 24381198
Results indicate that PLEKHA7 plays a cooperative role with nectin and afadin in the proper formation of Adherens junction (AJ) in epithelial cell. PMID: 23990464
This study demonstrated that nectin1- and nectin3-mediated interactions between Cajal-Retzius cells cells and migrating neurons are critical for radial migration. PMID: 23931996
Cell adhesion molecule nectin-3 is required for the effects of corticotropin releasing hormone receptor (CRHR)1 on cognition and structural remodeling after early-life stress exposure. PMID: 23644483
Nectin-3, which forms hetero-trans-dimers with nectin-1, undergoes intramembrane cleavage mediated by PS1/gamma-secretase. PMID: 21910732
nectin-afadin system plays essential roles in coupling cell-cell adhesion and the cortical actin scaffold in spermatids at Sertoli-spermatid junctions and in subsequent sperm morphogenesis PMID: 12121624
Overexpressed nectin recruited N-cadherin DN to the nectin-based cell-cell adhesion sites in an afadin-dependent manner. PMID: 12686612
the possible trans-interaction between the bona fide cell-cell adherens type adhesion system (cadherin/nectin) and the cell-matrix adhesion system (integrin/CD155) by virtue of their nectin-3 and CD155 components, respectively. PMID: 12759359
Nectin-3 does not have a role in Necl-5/Tage4- enhanced cell migration PMID: 14871893
These results indicate that the heterophilic trans-interaction between nectin-2 and nectin-3 is essential for the formation and maintenance of Sertoli-spermatid junctions that plays a critical role in spermatid development. PMID: 16923130
Inactivated nectin-2 and nectin-3 disrupted the nectin-afadin-actin system, and finally the actin filaments disappeared. As a result, the specialization lost the holding function and detachment of spermatids was observed. PMID: 18097512
Nectin plays a novel role in the co-localization of JAM and claudin at the same cell-cell adhesion membrane domains. PMID: 18547333

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Subcellular Location

Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane.

Protein Families

Nectin family

Tissue Specificity

Ubiquitous with high expression in testes. Localized in spermatids at Sertoli-spermatid junctions. Expressed in ovarian granulosa cells, but only faintly expressed after ovulation.

Database Links

KEGG: mmu:58998

STRING: 10090.ENSMUSP00000023334

UniGene: Mm.328072

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Product Details

Customer Reviews and Q&A

Target Background

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