Recombinant Mouse Protein Jumonji (Jarid2), partial

Code CSB-YP733821MO
MSDS
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Source Yeast
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Code CSB-EP733821MO
MSDS
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Source E.coli
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Code CSB-EP733821MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733821MO
MSDS
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Source Baculovirus
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Code CSB-MP733821MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Jarid2
Uniprot No.
Alternative Names
Jarid2; Jmj; Protein Jumonji; Jumonji/ARID domain-containing protein 2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts as an accessory subunit for the core PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 (H3K27me3) trimethylation on chromatin. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells, thereby playing a key role in stem cell differentiation and normal embryonic development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. Does not have histone demethylase activity.
Gene References into Functions
  1. JARID2 regulates muscle differentiation by modulating Wnt signaling through direct repression of SFRP1. PMID: 30119689
  2. These findings ascribe a critical function to Jarid2 and define a key mechanism that links PRC1 and PRC2 in the establishment of Polycomb domains. PMID: 27892467
  3. findings demonstrate a critical role for Etv2-miR-130a-Jarid2 in vascular patterning both in vitro and in vivo PMID: 29232705
  4. study suggests that miR-155 plays a host-damaging role during dextran sulfate sodium-induced colitis mice and induces Th17 differentiation by targeting Jarid2 PMID: 28461115
  5. JARID2 physically interacts with ESRRB, SALL4A, and PRDM14 PMID: 26523946
  6. ESCs lacking Jarid2 show constitutive Nanog expression, reduced PCP/Wnt signaling and form more than one ICM upon injection to E3.5 mouse blastocysts. PMID: 26190104
  7. Jarid2 is a novel player in iNKT cell maturation that regulates PLZF expression by modulating H3K9 methylation PMID: 25105474
  8. Jarid2 is an important component of PRC2 that has a central role in coordinating hematopoietic stem and progenitor cell function PMID: 25645357
  9. miR-155 regulates the fate of Th17 and Treg cells and miR-155 contributes to Th17 cell function by suppressing the inhibitory effects of Jarid2. PMID: 24856900
  10. Data indicate that upon stimulation of muscle cells with IFN-gamma, JARID2 and other members of the Polycomb repressive complex 2 (PRC2) were recruited to promoters to repress myogenesis. PMID: 24327761
  11. Jarid2 exhibits nucleosome-binding activity that contributes to PRC2 stimulation PMID: 24352422
  12. Jarid2 (Jumonji, AT rich interactive domain 2) regulates NOTCH1 expression via histone modification in the developing heart. PMID: 22110129
  13. these studies identify JARID2, MTF2, and esPRC2p48 as important regulatory subunits of PRC2 in ES cells and reveal critical functions of these subunits PMID: 21732481
  14. Jarid2 is required for the scheduled proliferation of epidermal stem and progenitor cells necessary to maintain epidermal homeostasis. PMID: 21811233
  15. Endothelial Jarid2/Jumonji is required for normal cardiac development and proper Notch1 expression PMID: 21402699
  16. A Jmj-cyclin D1 pathway coordinately regulates proliferation and differentiation of cardiomyocytes. PMID: 21447557
  17. Jarid2 (a specific regulatory subunit of the Polycomb repressive complex 2) regulates function at bivalent genes in embryonic stem cells. (Review) PMID: 21213100
  18. Data from family-based linkage disequilibrium study confirmed the pivotal role of JARID2 in orofacial development and strongly supports a role for this gene in CL/P etiology. PMID: 20506229
  19. Findings highlight Jarid2 as one of several potential mediators of the critical role played by Nkx2.5 during OFT morphogenesis. PMID: 20549724
  20. Jarid2 associates with Polycomb-Repressive Complex 2 and stimulates its enzymatic activity in vitro PMID: 20123894
  21. Jarid2 promotes PRC2 recruitment to the target genes while inhibiting PRC2 histone methyltransferase activity, suggesting that it acts as a "molecular rheostat" that finely calibrates PRC2 functions at developmental genes. PMID: 20064375
  22. Study demonstrates that JMJ is a subunit of the PRC2 complex, colocalizes with PRC2 on chromatin, and regulates H3K27me3 modification by modulating PRC2 activity. PMID: 20064376
  23. Jml downregulates cardiac cell proliferation by repressing cyclin D1 expression. PMID: 12852854
  24. domains for transcriptional repression, DNA binding, and nuclear localization signal were mapped PMID: 12890668
  25. jumonji mutant mice have cardiac abnormalities and early lethality. PMID: 15504358
  26. Jumonji may play important roles in the down-regulation of ANF gene expression and in heart development PMID: 15542826
  27. JMJ has a role in cardiovascular development in mice [review] PMID: 15580614
  28. data suggest that jumonji down-regulates the cell growth via interaction with retinblastoma protein, which would provide important insights into the cardiac defects observed in jmj mutant mice PMID: 15870077
  29. jmj is mainly expressed in the cyclin D1 negative region in the hindbrain, and cyclin D1 expression in the ventricular zone was upregulated in jmj mutants. PMID: 17189626
  30. data suggest that JMJ may be a critical, previously unrecognized factor that mediates some of the cellular effects of NO (nitric oxide), that NO may be able to increase JMJ in diseases associated with reduced JMJ expression PMID: 18498724

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Subcellular Location
Nucleus. Note=Colocalizes with the PRC2 complex on chromatin.
Protein Families
JARID2 family
Tissue Specificity
Widely expressed in embryos. In adults, expressed at high levels in heart, skeletal muscle, brain and thymus.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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