Recombinant Mouse Protein Wnt-9b(Wnt9b)

Code CSB-YP026146MO
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Source Yeast
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Code CSB-EP026146MO
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Source E.coli
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Code CSB-EP026146MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026146MO
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Source Baculovirus
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Code CSB-MP026146MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Wnt9b
Uniprot No. O35468
Alternative Names Wnt9b; Wnt14b; Wnt15; Protein Wnt-9b; Protein Wnt-14b; Protein Wnt-15
Species Mus musculus (Mouse)
Expression Region 24-359
Target Protein Sequence AAYFGLT GREVLTPFPG LGTAAAPAQA GAHLKQCDLL KLSRRQKQLC RREPGLAETL RDAAHLGLLE CQFQFRQERW NCSLEGRTGL LQRGFKETAF LYAVSAAALT HALARACSAG RMERCTCDDS PGLESRQAWQ WGVCGDNLKY STKFLSNFLG PKRGSKDLRA RADAHNTHVG IKAVKSGLRT TCKCHGVSGS CAVRTCWKQL SPFRETGQVL KLRYDTAVKV SSATNEALGR LELWAPAKPG GPAKGLAPRP GDLVYMEDSP SFCRPSKYSP GTAGRVCSRD SSCSSLCCGR GYDTQSRMVV FSCHCQVQWC CYVECQQCAQ QELVYTCKR
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Gene References into Functions
  1. Data indicate that mutations in wingless-type MMTV integration site family, member 9B protein (WNT9B) were frequently associated with Mayer-Rokitansky-Kuster-Hauser syndrome (MRKHS). PMID: 26610373
  2. Epigenetic mechanism causes Wnt9b deficiency and nonsyndromic cleft lip and palate in the A/WySn mouse strain. PMID: 25257647
  3. Expression of Wnt9b in Six2-positive cells disrupts cell fate decisions in the kidney and the gastrointestinal tract. PMID: 22912798
  4. Epithelial Wnt7b and Wnt9b as possible ligands of Fzd1-mediated beta-catenin (Ctnnb1)-dependent (canonical) Wnt signaling in the undifferentiated ureteric mesenchyme PMID: 22833126
  5. Study has identified a previously unknown regulatory link between WNT9B and FGF signaling during lip and upper jaw development. PMID: 22461561
  6. Notch pathway activation can replace the requirement for Wnt4 and Wnt9b in mesenchymal-to-epithelial transition of nephron stem cells PMID: 21852398
  7. transgenic overexpression of Wnt9b in the ureteric bud causes reduced branching in multiple founder lines. PMID: 20702564
  8. expression in most but not all tissues of the 16.5-day embryo; no significant elevation of Wnt9b expression was detected in 29 mouse mammary tumor virus-induced tumors PMID: 12573259
  9. Wnt9b is a common organizing signal regulating diverse components of the mammalian urogenital system. PMID: 16054034
  10. Signals through the canonical Wnt signaling pathway to regulate midfacial development and lip fusion. PMID: 16496313
  11. The noncomplementation of clf1 and Wnt9b- confirms that clf1 is a mutation of the Wnt9b gene. PMID: 16998816
  12. Findings show that Wnt9b, produced by the kidney collecting ducts, nonautonomously regulates morphogenesis of the developing kidney tubules. PMID: 19543268

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Database Links

KEGG: mmu:22412

STRING: 10090.ENSMUSP00000018630

UniGene: Mm.215161

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