Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Pdk3

Uniprot No.

Q922H2

Research Area

Signal Transduction

Alternative Names

Pdk3; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 3; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 3

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

131-362aa

Target Protein Sequence

IEYKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHEDKKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.3kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Discover our Recombinant Mouse Pdk3, a meticulously engineered protein designed to support signal transduction research in mouse models. This product features the pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 3, a mitochondrial protein that plays a vital role in the regulation of pyruvate dehydrogenase complex activity. Our protein offering focuses on a partial region of Pdk3 with an expression region of 131-362aa, ensuring optimal performance and consistency for your research needs.

Derived from an E. coli expression system, our Mouse Pdk3 protein is fused with an N-terminal 6xHis tag, allowing for efficient purification and detection processes. With a purity greater than 90% as determined by SDS-PAGE, our recombinant protein is available in both liquid and lyophilized powder forms to cater to various experimental requirements. Rely on our precision-crafted Recombinant Mouse Pdk3 to deliver consistent and dependable results for your signal transduction research projects.

Target Background

Function

Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.

Gene References into Functions

demonstrate that the increased PDK3 activity in patient fibroblasts (PDK3(R158H)) leads to the attenuation of PDC through hyper-phosphorylation of E1 at selected serine residues PMID: 27388934

Subcellular Location

Mitochondrion matrix.

Protein Families

PDK/BCKDK protein kinase family

Database Links

KEGG: mmu:236900

STRING: 10090.ENSMUSP00000036604

UniGene: Mm.12775

Code	CSB-EP852852MO
Abbreviation	Recombinant Mouse Pdk3 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP852852MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pdk3. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP852852MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pdk3.
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Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial (Pdk3), partial

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