Recombinant Mouse RNA binding protein fox-1 homolog 3 (Rbfox3)

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Code CSB-EP806847MO
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP806847MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Rbfox3.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP806847MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Rbfox3.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Rbfox3
Uniprot No.
Research Area
Others
Alternative Names
Rbfox3; D11Bwg0517e; Hrnbp3; RNA binding protein fox-1 homolog 3; Fox-1 homolog C; Hexaribonucleotide-binding protein 3; Fox-3; Neuronal nuclei antigen; NeuN antigen
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-374aa
Target Protein Sequence
MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPPEHGMTLYTPAQTHPEQPGTEASTQPIAGTQTVPQADEAAQTDNQQLHPSDPTEKQQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFETSSDADRAREKLNGTIVEGRKIEVNNATARVMTNKKPGNPYANGWKLNPVVGTVYGPEFYAVTSFPYPTTGTAVAYRGAHLRGRGRAVYNTFRAAPPPPPIPTYGAALEQTLVKMPVPWAGLAPCPLPPQQTPEPAYPTSPAFPPLSCPFASRVVYQDGFYGAEIYGGYAAYRYAQPAAATAAAYSDSYGRVYAAADPYHHTIGPTATYSIGTMASLCRGGYSRFTPY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
47.6 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Gene References into Functions
  1. Rbfox3-/- mice displayed normal non-image and image forming functions. Taken together, our results suggest RBFOX3 is dispensable for visual function PMID: 29401485
  2. Results demonstrate the important role of RBFOX3 during neural development and maturation and show that abnormalities in synaptic structure and function occur in Rbfox3 homozygous knockout mice. These findings may offer mechanistic explanations for human brain diseases associated with dysfunctional RBFOX3. PMID: 27701470
  3. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. PMID: 26619789
  4. In vitro analyses revealed that Rbfox3 functions as a positive and a negative regulator at the stage of pri-miRNA processing to precursor miRNA (pre-miRNA). PMID: 25240799
  5. Rbfox3-dependent Numb alternative splicing plays an important role in the progression of neuronal differentiation during vertebrate development. PMID: 23420872
  6. the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism PMID: 21747913
  7. During the development of olfactory bulb and epithelium, NeuN is expressed only in mature neural cells migrating from margin area to the inner granule cells layer of olfactory bulb. PMID: 17608158
  8. NeuN is the Fox-3 gene product and its expression is restricted to neural tissues. PMID: 19713214
  9. Enzymatic dephosphorylation abolishes NeuN immunoreactivity, confirming that NeuN is indeed a phosphoprotein, and establishing that binding of the defining antibody depends on its state of phosphorylation. PMID: 15605376

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Subcellular Location
Nucleus. Cytoplasm.; [Isoform 1]: Nucleus.; [Isoform 4]: Cytoplasm.; [Isoform 5]: Nucleus.
Tissue Specificity
Widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord (at protein level). Not expressed in all neuronal cells within a region, in cerebellum, expression is absent in Purk
Database Links
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