Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rab7a

Uniprot No.

P51150

Alternative Names

Rab7a; Rab7; Ras-related protein Rab-7a

Species

Mus musculus (Mouse)

Expression Region

2-207

Target Protein Sequence

TSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA SAESCSC

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surface expression of ACE2. May play a role in PRPH neuronal intermediate filament assembly.

Gene References into Functions

Rab7 deficiency exacerbates the severity of acute pancreatitis by impairing the autophagic and endocytic pathways toward lysosomes. PMID: 28588238
High Rab7 expression is associated with tumor growth and metastasis. PMID: 28924047
WDR91 serves as a Rab7 effector that is essential for neuronal development by facilitating endosome conversion in the endosome-lysosome pathway. PMID: 28860274
Vps34 has a previously unknown role in regulating Rab7 activity and late endosomal trafficking. PMID: 27793976
Rab7 accumulated in GCase deficient cells, supporting the notion that lysosomal recycling is impaired. Since recombinant GCase can reverse ALR impairment, we anticipate that strategies to restore GCase activity in the brains of both sporadic patients with PD and those with GBA1 mutations will improve autophagy lysosomal pathway, preventing the accumulation of a-synuclein and spread of pathology. PMID: 27378698
The inhibition of autophagosome-lysosome fusion induced by SapM is dependent on the interaction between SapM and Rab7. PMID: 27609571
Prion infection impairs lysosomal degradation capacity by interfering with rab7 membrane attachment in neuronal cells. PMID: 26865414
photoreceptor outer segments vesicles engulfed through Lyar-dependent pathway were targeted to phagosomes and colocalized with phagosome marker Rab7 PMID: 25735755
B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses. PMID: 25740947
CREG1 deficiency influenced the maturation of lysosomes and reduced the espression of Rab7, which might be involved in CREG1-induced cardiomyocyte autophagy. PMID: 25774384
Tropheryma whipplei blocks the switch from Rab5 to Rab7. PMID: 24586722
ADRB2 stimulation has caused a marked increase in the autophagy-targeted lipid droplets (LD) for lysosomal degradation, which is dependent on the LD-associated RAB7. PMID: 23708524
Loss of rab7 is better tolerated in naive T cells than the loss of atg5. PMID: 23615463
Rab7 is required for LPS-stimulated lysosome tubulation in macrophages. PMID: 22909026
Signaling protein ras-related protein rab-7A and septin 8 levels were significantly higher in hippocampus of poor contextual fear conditioning extinguishers. PMID: 21770032
Rab7a is an interacting partner of cellular prion protein in neuronal cells. PMID: 21604690
Results suggest that Rab7 may play a role in secretory lysosome movement toward the centrosome by interacting with RILP to recruit the minus-end motor, dynein. PMID: 21438969
Valproic acid improves defective neurite formation in neuroblastoma cells regardless of which Charcot-Marie-Tooth disease-associated Rab7 mutant protein is expressed, an effect mediated via the c-Jun N-terminal kinase (JNK) signaling pathway. PMID: 20645406
A novel role is demonstrated for SP-A in modulating endolysosomal trafficking via Rab7b (but not Rab5 nor Rab11) in primary alveolar macrophages, and the biochemical pathways are defined. PMID: 21248257
although only TBC1D15/Rab7-GAP altered Rab7-GTP levels, both Rab7-GAP and mVps39 regulate lysosomal morphology and play a role in maintaining growth factor dependence PMID: 20363736
CD63, but not RILP, is recruited to the phagosomes in macrophages expressing the dominant negative form of Rab7. PMID: 20236428
Phosphatidylinositol 3-kinase modulates Rab5 and Rab7 recruitment to phagosomes PMID: 12640132
Results show a role for Rab7 in the final maturation of late autophagic vacuoles. PMID: 15340014
Therefore, Rab7 plays an essential role in axonal retrograde transport by controlling a vesicular compartment implicated in neurotrophin traffic. PMID: 17046692
cholesterol accumulation can have a detrimental effect on phagosome maturation by impairing the activation of Rab7 PMID: 18955491
the late endosome/lysosome-localized Rab7b can down-regulate TLR9-triggered proinflammatory cytokine and type I IFN production by impairing TLR9 signaling via promotion of TLR9 degradation PMID: 19587007

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Subcellular Location

Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein; Cytoplasmic side. Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Melanosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet. Endosome membrane. Cytoplasmic vesicle.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Widely expressed. High expression in liver, heart and kidney. Found in sensory and motor neurons.

Database Links

KEGG: mmu:19349

STRING: 10090.ENSMUSP00000092658

UniGene: Mm.333233

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Recombinant Mouse Ras-related protein Rab-7a (Rab7a)

Product Details

Customer Reviews and Q&A

Target Background

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