Recombinant Mouse Rho guanine nucleotide exchange factor 12(Arhgef12) ,partial

Code CSB-YP823184MO
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Source Yeast
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Code CSB-EP823184MO
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Source E.coli
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Code CSB-EP823184MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823184MO
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Source Baculovirus
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Code CSB-MP823184MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Arhgef12
Uniprot No. Q8R4H2
Alternative Names Arhgef12; Kiaa0382; Larg; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEF
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13 (By similarity).
Gene References into Functions
  1. We studied the function of LARG in murine and human megakaryocytes and platelets with Larg knockout (KO), shRNA-mediated knockdown and small molecule-mediated inhibition. We found that LARG is important for human, but not murine, megakaryocyte maturation. PMID: 27345948
  2. Data found an inverse relationship between the expression of miR-660 and ARHGEF12 in both gain- and loss-of-function studies: overexpression of miR-660 declined the mRNA and protein expressions of ARHGEF12 in C2C12 cells differentiation; however, knockdown of miR-660 had completely opposite results. PMID: 28106300
  3. This study demonstrated an almost 100-fold higher agonist sensitivity of the Galpha13 LARG interaction compared to the Galpha13 activation in the absence of LARG. PMID: 28465324
  4. LARG may play a housekeeper role in regulating constitutive RhoA activity. Its role in regulating platelet function may principally be through RhoA-independent pathways. PMID: 26334261
  5. The increase in LARG expression was accompanied by a significant increase in ACh-stimulated Rho kinase and ZIP kinase activities, and sustained muscle contraction PMID: 24740538
  6. Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study. PMID: 24106280
  7. PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. PMID: 23467409
  8. RhoGEF12 acts as an integrator of stretch-induced signaling cascades in cardiomyocytes and is an interesting new target for therapeutic intervention in patients with pressure overload-induced heart failure. PMID: 23530122
  9. LARG is activated by the Src family tyrosine kinase Fyn, whereas GEF-H1 catalytic activity is enhanced by ERK downstream of a signalling cascade that includes FAK and Ras PMID: 21572419
  10. new insight into the mechanism by which p115RhoGEF is activated by Galpha(13). PMID: 21507947
  11. S1PR2-dependent activation of RhoA in smooth muscle cells is mediated by LARG and that this signaling mechanism promotes the differentiated SMC phenotype. PMID: 20702813
  12. Leukemia-associated Rho guanine nucleotide exchange factor promotes G alpha q-coupled activation of RhoA (larg protein) PMID: 12024019
  13. We demonstrate colocalization of the LARG protein with the IGF-1 receptor, suggesting a potential physiologic role for LARG as an activator of RhoA in response to IGF-1. PMID: 14513355
  14. mLARG is highly expressed in hematopoietic stem cell fractions, as well as in immature erythroid cells, and provide further support to the hypothesis of the potential involvement of LARG in regulation of early stages of hematopoiesis. PMID: 15276214
  15. Galpha(12/13) proteins exert essential functions linking extracellular signals to microtubule dynamics and cell polarity via RhoGEF and formin activity. PMID: 17959834
  16. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension PMID: 18084302
  17. IQ-ArfGEF/BRAG1 forms a postsynaptic protein complex containing PSD-95 and NMDA receptors at excitatory synapses, where it may function as a GEF for Arf6. PMID: 18164504
  18. Rho activation induces mDia1 activity and the latter potentially enables a positive feedback regulatory circuit by promoting LARG-driven activation of Rho and Rho targets such as Rho-associated coiled-coil forming kinase 1 (ROCK). PMID: 19265163
  19. The immunohistochemically demonstrated that IQ-ArfGEF/BRAG1 was associated with the synaptic ribbons of photoreceptors using both light and electron microscopy. Furthermore, we showed that IQ-ArfGEF/BRAG1 forms a complex with RIBEYE PMID: 19811534

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Subcellular Location Cytoplasm, Membrane
Tissue Specificity Expressed in brain, predominantly in neuronal cell bodies.
Database Links

STRING: 10090.ENSMUSP00000126598

UniGene: Mm.275266

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