Product Details

Purity

>85% (SDS-PAGE)

Target Names

Sh2d2a

Uniprot No.

Q9QXK9

Alternative Names

Sh2d2a; Lad; Ribp; SH2 domain-containing protein 2A; Lck-associated adapter protein; Lad; Rlk/Itk-binding protein; Ribp

Species

Mus musculus (Mouse)

Expression Region

1-374

Target Protein Sequence

MEFCLAQPCP QGNHEATSST FNTFQPMNLT QGRCQNLSCG SRPSMQVMKE QGVQLSPRTN HTVVSASAPG TAWVLGNADR AEEVPGKGDL SLQAETRAWV QKTQAHWLLL KTAPLWFHGF ITRREAERLL QPQPLGCYLV RFSESAVTFV LSYRSQTCCR HFLLAQLGDG RHVVLGEDSA HAQLQDLLEH YTECPLSPYG EILTQPLARQ TAEPAGLSLR ADSDSGSKRQ DPDTQLSLLL QQGQAQASGH TEKVWASQQK ATSQASRPRP PIPAKPQLPP EVYTSPASRL HQAPPINPIY QEPDEPIAFY AMGRGSPGDA PSNIYAEVEG PSGTAPIGHP ILRKCWSRPI SRGQVREVQG KISSRSRAER GSPS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Could be a T-cell-specific adapter protein involved in the control of T-cell activation. May play a role in p56-LCK-mediated T-cell signaling. Could be involved in the regulation of responses to T-cell activation stimuli, specifically proliferation and lymphokine production. Interactions with ITK and TXK may provide important biochemical links of these two important kinases with other components in the T-cell activation machinery.

Gene References into Functions

Sh2d2a protein deficient mice display reduced clearance of Dm157 MCMV infection in the spleen. PMID: 25783199
The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192. PMID: 25492967
Results suggest a modulatory role for T cell specific adapter protein SH2D2A in T cell mediated immune surveillance of cancer. PMID: 23144743
results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells. PMID: 19756394
role of TSAd as a critical regulator of T cell death whose absence promotes systemic autoimmunity PMID: 12953096
TSAd appears to contribute to interleukin-2 synthesis at multiple different levels PMID: 15752554
The major role of T cell-specific adapter protein in peripheral T cell cytoplasm is for activation of LCK protein tyrosine kinase at the outset of T cell receptor signal transduction PMID: 16446380
Although Rlk/Itk-binding protein (RIBP) is coexpressed with related adaptor lymphocytes of unknown function X (ALX) protein on both T- and B-lymphocytes during cell development, no redundancy or synergy is found. PMID: 17641043

Hide All

Subcellular Location

Cytoplasm. Cell membrane. Note=Redistributed from cytoplasm to the plasma membrane in a T-cell activation-dependent manner.

Tissue Specificity

Expression limited to tissues of the immune system and, in particular, activated T-cells and natural killer cells. Expressed in the thymus, lymph node, and to a lesser extent, in the spleen and bone marrow. According to PubMed:10553045, also expressed in

Database Links

KEGG: mmu:27371

STRING: 10090.ENSMUSP00000103207

UniGene: Mm.86361

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP868766MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP868766MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP868766MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP868766MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP868766MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse SH2 domain-containing protein 2A (Sh2d2a)

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage