Recombinant Mouse Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (Ppp2r1a)

Code CSB-EP758842MO
Abbreviation Recombinant Mouse Ppp2r1a protein
MSDS
Size $224
Order now
Image
  • Warning: preg_replace(): Unknown modifier 'a' in /www/wwwroot/cusabio.com/caches/caches_template/20141113/content/show_product_protein.php on line 156
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Signal Transduction
Alternative Names
PP2A subunit A isoform PR65-alpha;PP2A subunit A isoform R1-alpha
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
2-589aa
Target Protein Sequence
AAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMTQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
72.1 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
Gene References into Functions
  1. oocyte-specific deletion of Ppp2r1a led to severe female subfertility without affecting follicle survival, growth, and ovulation. PMID: 24899574
  2. PP2A-A alpha transcriptional regulation is mediated by multiple factors including AP-2alpha, CREB, ETS-1, and SP-1 PMID: 19750005
  3. protein phosphatase 2A overexpression in NIH 3T3 cells PMID: 11665638
  4. T-cell receptor (TCR)-dependent tyrosine phosphorylation may be the mechanism by which the regulatory subunit of PP2A prevents the inhibitory function of CTLA-4, before TCR-CTLA-4 coligation. PMID: 11994459
  5. PP-2A regulation of paxillin phosphorylation may have a role in controlling tumor cell adherence and motility. PMID: 12198769
  6. PP2A may contribute to melanoma cell radioresistance: the truncated isoform of the PP2A B56gamma regulatory subunit reduces irradiation-induced Mdm2 phosphorylation PMID: 15024700
  7. PP2A has a fundamental role in cardiac function PMID: 15247211
  8. response of endothelial cells to the tumor-derived motility-stimulatory factors PGE2/TGFbeta involved a decline in the activity of PP2A, a loss of PP2A co-precipitation with PTEN, and an increase in the PTEN serine phosphorylation level. PMID: 15516833
  9. heat treatment inactivates PP2A, which may subsequently cause ERK activation and decreases melanin synthesis in melanocytes PMID: 15894174
  10. Results indicate that polyamines regulate protein phosphatase 2A (PP2A)activity, and inhibition of PP2A in response to polyamine depletion increases levels of Bad and Bcl-2 proteins and prevents cytochrome c release, caspase-9, and caspase-3 activation. PMID: 15994315
  11. Pim on PP2A activity may mediate the levels of c-Myc and the phosphorylation of proteins needed for increased protein synthesis, which could have a significant impact on tumor growth PMID: 16123140
  12. PP2A plays a positive rather than a negative role in the regulation of IKKbeta PMID: 16126728
  13. the carboxy-terminal leucine L309 of the PP2A catalytic subunit determines PP2A heterotrimer composition in vivo. PP2A subunit composition plays a crucial role in regulating cell adhesion and as a consequence in the development of the Harderian gland. PMID: 16679006
  14. PP2A-mediated dephosphorylation of BCL-2 is required to protect BCL-2 from proteasome-dependent degradation, affecting resistance to ER stress PMID: 16717086
  15. PP2A is involved in the post-transcriptional regulation of tumor necrosis factor-alpha. PMID: 17170118
  16. Akt dephosphorylation was mediated by ceramide-induced PKCzeta-Akt association and PP2A activation in Leishmania donovani-infected macrophages. PMID: 17220321
  17. Based on crystal structures of Galpha(12) and PP2A Aalpha, a model describing the binding surfaces and potential mechanisms of Galpha(12)-mediated PP2A activation is presented. PMID: 17303700
  18. protein phosphatase 2a recruitment to I-kappaB kinase gamma/NF-kappaB essential modulator is regulated by heptad repeats, which are targeted by HTLV-I tax PMID: 17314097
  19. Suppression of PP-2A damages intracellular transport and leads to cell degeneration, and the memory deficits induced by suppression of the phosphatases. PMID: 17472709
  20. Results describe the crystal structure of full-length SV40 small t antigen in complex with protein phosphatase 2A A subunit at 3.1 A resolution. PMID: 17608567
  21. These results indicate a role for GSK-3beta in ceramide-induced apoptosis, in which GSK-3beta acts downstream of PP2A and the PI 3-kinase-Akt pathway, and upstream of caspase-2 and caspase-8. PMID: 17666435
  22. C3G triggers PP2A activation and binding to MEK and ERK at the subcortical actin cytoskeleton, thus favouring ERK dephosphorylation. PMID: 17825818
  23. Polyoma small T uses PP2A to promote phosphorylation of Akt on both T308 & S473, resulting in a different pattern of phosphorylation of Akt substrates & shifting Akt from a proapoptotic to an antiapoptotic mode. PMID: 18006659
  24. These data show that A(2A)R and A(1)R regulate PP2A activity, thus suggesting an important mechanism for modulating myocardial contractility. PMID: 18181173
  25. PP2A is a physiologic phosphatase of FOXO1. PMID: 18211894
  26. a role for the demethylated form of PP2A in maintenance of enzyme function and phosphorylation networks in vivo. PMID: 18596935
  27. Nitration in PP2Ac is correlated with PP2A activation that mediates endothelial barrier dysfunction. PMID: 18791203
  28. B56epsilon is alternatively translated adds a new level of regulation to PP2A holoenzymes PMID: 19129191
  29. Inhibition of serine/threonine phosphatase PP2A enhances cancer chemotherapy by blocking DNA damage induced defense mechanisms. PMID: 19564615
  30. Knockdown of PP2A inhibits forskolin-induced nuclear translocation of HDAC4 and attenuates the ability of this signaling molecule to repress collagen X expression in chondrocytes. PMID: 19704004
  31. Study describes a novel Akt-dependent Pp2a activity that acts at least partly through Jun to affect initial barrier formation during late embryonic epidermal development. PMID: 19762425

Show More

Hide All

Subcellular Location
Cytoplasm. Nucleus. Chromosome, centromere. Lateral cell membrane. Cell projection, dendrite.
Protein Families
Phosphatase 2A regulatory subunit A family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*