Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Tnc

Uniprot No.

Q80YX1

Research Area

Signal Transduction

Alternative Names

Tnc; Hxb; Tenascin; TN; Hexabrachion; Tenascin-C; TN-C

Species

Mus musculus (Mouse)

Source

Baculovirus

Expression Region

1884-2099aa

Target Protein Sequence

GLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.8 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The gene responsible for the Mouse Tnc protein (1884-2099aa) is cloned into a plasmid vector to obtain recombinant plasmid, which is then transformed into baculovirus cells. baculovirus cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to endure a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. Post-expression, affinity purification is employed to isolate and purify the recombinant Mouse Tnc protein from the cell lysate. The resulting recombinant protein is subjected to denaturing SDS-PAGE, allowing for an estimation of its purity, surpassing 85%.

Target Background

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth when provided to neurons in culture. May play a role in supporting the growth of epithelial tumors. Ligand for integrins ITGA8:ITGB1, ITGA9:ITGB1, ITGAV:ITGB3 and ITGAV:ITGB6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells.

Gene References into Functions

TNC causes post-subarachnoid hemorrhage neuronal apoptosis and neuroinflammation. PMID: 29546590
Results identify tenascin-C as an endogenous danger signal that is upregulated in systemic sclerosis and drives TLR4-dependent fibroblast activation, and by its persistence impedes fibrosis resolution. PMID: 27256716
ATF3 promotes macrophage migration and reverses M1polarized macrophages to the M2 phenotype by upregulation of TNC via the Wnt/betacatenin signaling pathway. PMID: 28714032
housing of TnC-/- mice in enriched environment abolished hyperlocomotion, led to faster habituation to novel environment, strengthened the grasp of fore limbs and partially improved movement coordination, while the swimming ability remained deficient PMID: 28549651
The synaptic plasticity occurs in the hippocampus of freely behaving mice that lack tenascin-C, the informational content stored by synaptic plasticity is not the same. PMID: 28512860
Study suggests that tenascin-C (TnC) contributes to the regulation of structural plasticity in the cerebellum and that interactions between TnC and matrix metalloproteinase 9 are likely to be important for these processes to occur. PMID: 27089885
the exact role of TNC in primary tumor growth PMID: 28874093
A deficiency of tenascin C interferes with Th1 and Th17 cells, protecting animals from experimental autoimmune encephalomyelitis. PMID: 27974153
secreted by transdifferentiated retinal pigment epithelial cells and promotes the development of choroidal neovascularization via integrin alphaV in a paracrine manner PMID: 27668890
Tenascin-C may be an important mediator in the development of brain edema and blood-brain barrier disruption following subarachnoid hemorrhage, mechanisms for which may involve MAPK-mediated MMP-9 induction and ZO-1 degradation PMID: 26473781
TN-C aggravates autoimmune myocarditis by driving the dendritic cell activation and Th17 differentiation via toll-like receptor 4. PMID: 25376187
TNC supports the stress-induced expression of extracellular matrix that reinforce the aorta and the stress-induced excessive inflammatory response in the aorta. PMID: 24514259
TNC plays an important role in TMJ wound healing, especially for wounds generated by mechanical stress. PMID: 25578971
Tenascin-C is required for normal Wnt/beta-catenin signaling in the whisker follicle stem cell niche. PMID: 25196097
TNC downregulates Dickkopf-1 (DKK1) promoter activity through the blocking of actin stress fiber formation, activates Wnt signaling, and induces Wnt target genes in tumor and endothelial cells. PMID: 24139798
The increased levels of elastin, type V collagen and tenascin C are probably the result of increased expression by fibroblastic cells; reversely, elastin influences myofibroblast differentiation PMID: 24291458
Elevated tenascin-C expression in globoid cell leukodystrophy modified microglial functional response to psychosine. PMID: 25192051
Tenascin-C-derived peptide TNIIIA2 highly enhances cell survival and platelet-derived growth factor (PDGF)-dependent cell proliferation through potentiated and sustained activation of integrin alpha5beta1. PMID: 24808173
FHL2-deficient mice developed a severe and long-lasting lung pathology due to enhanced expression of tenascin C and impaired activation of inflammation-resolving macrophages. PMID: 24260575
the CD34-positive whisker follicle stem cell niche contains both tenascin-C and tenascin-W, and these glycoproteins might play a role in directing the migration and proliferation of these stem cells PMID: 24101721
It is upregulated in inflammation and induces further inflammatory responses and the functional inhibition exerts beneficial effects on AD pathogenesis, suggesting a potential for tnc as a new therapeutic target in AD. PMID: 23673309
Tenascin C did not play an essential role in stem and progenitor cell homing to BM, but significantly altered lymphoid primed progenitor cell homing PMID: 24084079
TNC does not appear to contribute directly to outflow resistance in the eye. PMID: 23882691
TNC expression controls eotaxin level in apo E-/- mice; this chemokine plays a key role in the development of atherosclerosis PMID: 23433402
This study provided evidence that the tenascin-c modulates synaptogenesis and long-term synapse stability. The mutant neurons display reduced frequencies of mEPSCs and mIPSCs. PMID: 23637166
cleavage by gingipains directly affects the biological activity of both fibronectin and tenascin-C in a manner that might lead to increased cell detachment and loss during periodontal disease. PMID: 23313574
Tenascin-C enables macrophage translation of proinflammatory cytokines upon lipopolysaccharide activation of toll-like receptor 4 (TLR4) and suppresses the synthesis of anti-inflammatory cytokines. PMID: 23084751
These data unveil a protective role for tenascin C (TNC) in atherosclerosis and suggest that TNC signaling may have the potential to reduce atherosclerosis, in part by modulating VCAM-1 expression. PMID: 22300502
TNC is involved in the etiopathology of obesity via visceral adipose tissue inflammation representing a link with extracellular matrix remodeling. PMID: 22851489
Both cycling G2-phase cells and early post-mitotic neurons were significantly increased in the retina due to Tnc-deficiency. Further investigations suggested that Tnc regulates these processes via the Wnt-signaling cascade. PMID: 22691363
This study provided evidence that the tenascin-C affect the repair of the blood-spinal cord barrier repair in soinal cord injury. PMID: 22473292
Mice lacking TN-C (TN-C(-/-)) mice showed normal steady-state hematopoiesis; however, they failed to reconstitute hematopoiesis after bone marrow ablation and showed high lethality. PMID: 22553313
data establish a role for BMP, Wnts, and mechanical loading in the regulation of tenascin expression in osteoblasts PMID: 21751239
Tnc deficiency interfered with VCAM-1 vascular deposition and down-regulation of PECAM-1, disrupted MMP-9-positive leukocyte infiltration, hampered apoptosis and necrosis, and favored liver repair/regeneration after ischemia-reperfusion injury. PMID: 21898491
TN-C may be a useful biomarker for indicating the pathological status of smooth muscle cells and interstitial cells in abdominal aortic aneurysm. PMID: 21951663
The data implicated tenascin C in the regulation of proliferation and lineage progression of astroglial progenitors in specific domains of the developing spinal cord. PMID: 22071102
Data show that reduction in metastasis due to the loss of S100A4(+) fibroblasts correlated with a concomitant decrease in the expression of several ECM molecules and growth factors, particularly Tenascin-C and VEGF-A. PMID: 21911392
Promoter-reporter and chromatin immunoprecipitation experiments unraveled a SAP-dependent, SRF-independent interaction of MKL1 with the proximal promoter region of TNC. PMID: 21705668
Tenascin-C is expressed in neural stem cells and neural cells derived from embryonic stem cells where it is expected to have a specific functional role in facilitating nervous tissue repair and regeneration. PMID: 20689858
Study suggests a new role of tenascin-C as a regulator of the fibrinolytic system. PMID: 21354146
Overexpression of TNC-fnD via adeno-associated virus in wild-type mice improved locomotor recovery, increased monaminergic axons sprouting, and reduced lesion scar volume after spinal cord injury. PMID: 20606643
HNK-1 epitope-carrying tenascin-C spliced variant regulates the proliferation of mouse embryonic neural stem cells. PMID: 20855890
Tenascin-C is required for injury-induced epithelial-mesenchymal transition in the mouse lens epithelium. PMID: 20664686
Mechanisms are presented of the first signaling pathways that underlie Tnc-induced, extracellular matrix-dependent maintenance of the immature state of oligodendendrocyte precursor cells. PMID: 20844127
Tenascin-C may thus accelerate adverse ventricular remodeling, cardiac failure, and fibrosis in the residual myocardium after myocardial infarction. PMID: 20081106
Here we show that periostin, a matricellular protein, promotes incorporation of tenascin-C into the extracellular matrix and organizes a meshwork architecture of the extracellular matrix. PMID: 19887451
These findings indicate a role for tenascin-C in structural organization of the CA1 hippocampal subfield and in shaping neural activity. PMID: 19280660
results suggested that the isoforms containing one or five alternatively spliced domains play important roles in the healing process of glomerulonephritis PMID: 12009782
TN-C is involved in hippocampus-dependent contextual memory and synaptic plasticity; the FN6-8 domain is one of molecular determinants mediating these functions. PMID: 12359159
TN-C induces MMP-9 expression directly and by collaboration with TGF-beta in breast cancer progression PMID: 12672030

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Tenascin family

Tissue Specificity

Expressed in kidney, aortic valve, corneal limbus, periosteum around the ribs, cerebellum, stomach and intestine. High levels of isoform 2 in lung and brain of newborn mice. High levels of isoform 5 in thymus, moderate levels in brain of newborn and adult

Database Links

KEGG: mmu:21923

UniGene: Mm.454219

Code	CSB-BP768917MO
Abbreviation	Recombinant Mouse Tnc protein, partial
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Tenascin (Tnc), partial

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