Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ubqln2

Uniprot No.

Q9QZM0

Alternative Names

Ubqln2; Plic2Ubiquilin-2; Chap1; DSK2 homolog; Protein linking IAP with cytoskeleton 2; PLIC-2; Ubiquitin-like product Chap1/Dsk2

Species

Mus musculus (Mouse)

Expression Region

2-638

Target Protein Sequence

AENGESSGP PRPSRGPAAA PGAASPPAEP KIIKVTVKTP KEKEEFAVPE NSTVQQFKEA ISKRFKSQTD QLVLIFAGKI LKDQDTLMQH GIHDGLTVHL VIKSQNRPQG QATTQPSTTA GTSTTTTTTT TAAAPAATTS SAPRSSSTPT TTNSSSFGLG SLSSLSNLGL NSPNFTELQN QMQQQLLASP EMMIQIMENP FVQSMLSNPD LMRQLIMANP QMQQLIQRNP EISHLLNNPD IMRQTLEIAR NPAMMQEMMR NQDLALSNLE SIPGGYNALR RMYTDIQEPM LNAAQEQFGG NPFATVGSSS TSGEGTQPSR TENRDPLPNP WAPPPTTQTA ATTTTTTTTS SGSGSGSSSS STTAGNTMAA ANYVASIFST PGMQSLLQQI TENPQLIQNM LSAPYMRSMM QSLSQNPDMA AQMMLSSPLF TSNPQLQEQM RPQLPNFLQQ MQNPETIAAM SNPRAMQALM QIQQGLQTLA TEAPGLIPSF APGVGMGVLG TAITPVGPVT PIGPIGPIVP FTPIGPIGPI GPTGPASSPG STGTGIPPAT TVSSSAPTET ISPTSESGPN QQFIQQMVQA LTGGSPPQPP NPEVRFQQQL EQLNAMGFLN REANLQALIA TGGDINAAIE RLLGSQPS

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs). Links CD47 to vimentin-containing intermediate filaments of the cytoskeleton.

Gene References into Functions

Results have shown that the two P497S and P506T mutant UBQLN2 Tg mouse lines recapitulate the central features of ALS with frontotemporal dementia (ALS-FTD) found in humans and indicate that these mutant UBQLN2 mice with end stage of disease display a reduction of TDP-43 staining in the nucleus with concomitant formation of cytoplasmic ubiquitin+ inclusions in spinal motor neurons. PMID: 27834214
UBQLN2 most evidently bound to HSP70-type chaperones, UBQLN1 and UBQLN4 and to a lesser extent to proteasomal subunits; UBQLN2 may be involved in the regulation of misfolded proteins. Mutations in UBQLN2, which lead to neurodegeneration in humans, are defective in chaperone binding, impair aggregate clearance, and cause cognitive deficits in mice. PMID: 27477512
Results showed that UBQLN2 is selectively recruited to nuclear inclusions in Huntington's disease but not spinocerebellar ataxia type 3 PMID: 26141599
UBQLN2 dysregulation in neurons can drive NF-kappaB activation and cytosolic TDP-43 aggregation. PMID: 26521126
Transduction of UBQLN2 mutants (P497H, P497S, and P506T) induce proteinopathy and cause behavioral deficits, supporting a "toxic" gain-of-function amyotrophic lateral sclerosis model, in mice. PMID: 26152284
results were confirmed by similar findings for ubiquilin-1 and -2 in human brain tissue sections, where accumulation was observed in huntingtin inclusions PMID: 23774650
UBQLN2-transfected cells did not show increased starvation-induced cell death. PMID: 19148225

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Subcellular Location

Cytoplasm. Nucleus. Membrane. Cytoplasmic vesicle, autophagosome.

Tissue Specificity

Highly expressed in smooth muscle. Expression in other tissues is very low.

Database Links

KEGG: mmu:54609

STRING: 10090.ENSMUSP00000056888

UniGene: Mm.430772

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Recombinant Mouse Ubiquilin-2 (Ubqln2)

Product Details

Customer Reviews and Q&A

Target Background

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