Recombinant Mouse Uromodulin (Umod), partial

Code CSB-EP025616MO
MSDS
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Source E.coli
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Code CSB-EP025616MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025616MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP025616MO
MSDS
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Source Mammalian cell
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Umod
Uniprot No.
Alternative Names
Umod; Uromodulin; Tamm-Horsfall urinary glycoprotein; THP) [Cleaved into: Uromodulin; secreted form]
Species
Mus musculus (Mouse)
Expression Region
25-588
Target Protein Sequence
NSTEARRCSECHNNATCTVDGVVTTCSCQTGFTGDGLVCEDMDECATPWTHNCSNSSCVNTPGSFKCSCQDGFRLTPELSCTDVDECSEQGLSNCHALATCVNTEGDYLCVCPEGFTGDGWYCECSPGSCEPGLDCLPQGPDGKLVCQDPCNTYETLTEYWRSTEYGVGYSCDAGLHGWYRFTGQGGVRMAETCVPVLRCNTAAPMWLNGSHPSSSEGIVSRTACAHWSDQCCRWSTEIQVKACPGGFYIYNLTAPPECNLAYCTDPSSVEGTCEECRVDEDCISDNGRWRCQCKQDSNITDVSQLEYRLECGANDIKMSLRKCQLQSLGFMNVFMYLNDRQCSGFSESDERDWMSIVTPARNGPCGTVLRRNETHATYSNTLYLANAIIIRDIIIRMNFECSYPLDMKVSLKTSLQPMVSALNISLGGTGKFTVRMALFQSPTYTQPHQGPSVMLSTEAFLYVGTMLDGGDLSRFVLLMTNCYATPSSNSTDPVKYFIIQDSCPRTEDTTIQVTENGESSQARFSVQMFRFAGNYDLVYLHCEVYLCDSTSEQCKPTCSGTRF
Protein Length
partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.; In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals.
Gene References into Functions
  1. these data support THP as an important negative regulator of neutrophil activation in the urinary tract, with dual functions to counteract bacterial colonization and suppress excessive inflammation within the urinary tract PMID: 28829050
  2. this study shows that uromodulin-SlpA binding dictates Lactobacillus acidophilus uptake by intestinal epithelial M cells PMID: 28992252
  3. results strongly suggest that the progressive worsening of kidney functions reflects the accumulation of the deleterious effects of the misfolded mutant THP and the compensatory responses PMID: 29145399
  4. Endoplasmic reticulum stress and apoptosis are key features in a mouse model of kidney disease driven by the uromodulin p.Cys147Trp mutation. PMID: 28990932
  5. Consistently, pathway enrichment analysis indicates that mutant uromodulin expression affects ER function and protein homeostasis. PMID: 28437467
  6. we show that Tamm-Horsfall protein deficiency stimulates proximal epithelial activation of the Interleukin-23/Interleukin-17 axis and systemic neutrophilia. PMID: 25556169
  7. AGP was more effective in limiting hepatic inflammation and maintaining perfusion than saline or HAS, in both endotoxemic and septic mice. AGP sequestration of LPS may contribute to its anti-inflammatory effects. PMID: 23941548
  8. UMOD upregulates TRPV5 by decreasing caveolin-1 dependent endocytosis of TRPV5. PMID: 23466996
  9. UMOD regulates sodium uptake in the thick ascending limb of the loop of Henle by modulating the effect of tumor necrosis factor-alpha on NKCC2A expression, making UMOD an important determinant of blood pressure control. PMID: 24324041
  10. Genetic susceptibility link to hypertension and chronic kidney disease to uromodulin expression and uromodulin's effect on salt reabsorption in the kidney. PMID: 24185693
  11. Using an ischemia-reperfusion model of murine acute kidney injury, we show that, while THP expression in thick ascending loop is downregulated at the peak of injury, it is significantly upregulated 48 h after. PMID: 23389456
  12. Quercetin has the uricosuric and nephroprotective actions mediated by regulating the expression levels of renal organic ion transporters and UMOD. PMID: 21909718
  13. a unifying model that underscores the role of THP as a major regulator of renal and systemic immunity. PMID: 22451664
  14. Molecular and cellular effects of Tamm-Horsfall protein mutations and their rescue by chemical chaperones. PMID: 22117067
  15. Deletion of the von Hippel-Lindau protein (Vhl), which mediates HIF degradation under normoxia, using Tamm-Horsfall protein (Thp)-driven Cre expression showed strong expression of HIF-1alpha in thick ascending limb. PMID: 21921145
  16. THP-null mouse may have a distal tubular defect and thus mimics ileostomy patients with low urine volumes and acidic urine. PMID: 21063698
  17. THGP modulation of ROMK function confers a new role of THGP on renal ion transport and may contribute to salt wasting observed in FJHN/MCKD-2/GCKD patients. PMID: 21081491
  18. These data clearly demonstrate a gain-of-toxic function of uromodulin mutations providing insights into the pathogenetic mechanism of the disease. PMID: 20472742
  19. Normal Tamm-Horsfall protein (THP) plays an important role in defending the urinary system against calcification. Reduced expression and/or decreased function of THP could contribute to nephrolithiasis. PMID: 20591941
  20. proximal 5'-flanking sequence is sufficient to drive the kidney-specific expression of a heterologous reporter gene PMID: 11880321
  21. potential THP defects, either quantitative or qualitative, could predispose the urinary bladder to bacterial infections PMID: 14665435
  22. THP serves as a soluble receptor for type 1 fimbriated E. coli and helps eliminate bacteria from the urinary tract. PMID: 14871399
  23. THP is a critical urinary defense factor and its deficiency could be an important contributing factor in nephrolithiasis PMID: 15327412
  24. Tamm-Horsfall glycoprotein plays an important regulatory role in the kidney PMID: 15522986
  25. The genes encoding pre-pro-epidermal growth factor (pre-pro-EGF) and Tamm-Horsfall protein (THP)/uromodulin were aberrantly expressed in the kidneys of COX-2 -/- mice at all stages of their development. PMID: 15741608
  26. Whole urine from osteopontin, Tamm-Horsfall protein , or double-null mice all possessed a dramatically reduced ability to inhibit the adhesion of calcium oxalate monohydrate crystals to renal epithelial cells PMID: 17898038
  27. Urinary uromodulin is generated by a conserved C-terminal proteolytic cleavage and retains its entire ZP domain. PMID: 18375198
  28. The finding that LZP might act as a new partner of THP would provide novel insights into renal functions related to THP and LZP, such as the urothelial permeability barrier and the host defense against the adhesion of pathogens. PMID: 18830570
  29. Letter: Tamm-Horsfall protein knockout mice have increased stress induced micturition. PMID: 19214995
  30. Umod might act as a gain-of-toxic-function mutation PMID: 19692485

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Subcellular Location
[Uromodulin, secreted form]: Secreted.; Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane.
Tissue Specificity
Detected in urine (secreted form). Detected in kidney thick ascending limb epithelial cells (at protein level).
Database Links
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