Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Umod

Uniprot No.

Q91X17

Research Area

Others

Alternative Names

Umod; Uromodulin; Tamm-Horsfall urinary glycoprotein; THP) [Cleaved into: Uromodulin; secreted form]

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

25-588aa

Target Protein Sequence

NSTEARRCSECHNNATCTVDGVVTTCSCQTGFTGDGLVCEDMDECATPWTHNCSNSSCVNTPGSFKCSCQDGFRLTPELSCTDVDECSEQGLSNCHALATCVNTEGDYLCVCPEGFTGDGWYCECSPGSCEPGLDCLPQGPDGKLVCQDPCNTYETLTEYWRSTEYGVGYSCDAGLHGWYRFTGQGGVRMAETCVPVLRCNTAAPMWLNGSHPSSSEGIVSRTACAHWSDQCCRWSTEIQVKACPGGFYIYNLTAPPECNLAYCTDPSSVEGTCEECRVDEDCISDNGRWRCQCKQDSNITDVSQLEYRLECGANDIKMSLRKCQLQSLGFMNVFMYLNDRQCSGFSESDERDWMSIVTPARNGPCGTVLRRNETHATYSNTLYLANAIIIRDIIIRMNFECSYPLDMKVSLKTSLQPMVSALNISLGGTGKFTVRMALFQSPTYTQPHQGPSVMLSTEAFLYVGTMLDGGDLSRFVLLMTNCYATPSSNSTDPVKYFIIQDSCPRTEDTTIQVTENGESSQARFSVQMFRFAGNYDLVYLHCEVYLCDSTSEQCKPTCSGTRF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

64.2kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The Recombinant Mouse Umod is a superior quality recombinant protein tailored for researchers working on Uromodulin studies. This protein mirrors the Uromodulin, also acknowledged as Tamm-Horsfall urinary glycoprotein or THP, in the Mouse species. Crafted using a Yeast expression system, this partial protein ensures faithful representation of the natural protein, providing trust in your experimental results.

Extending from the 25th to the 588th amino acid sequence, the Recombinant Mouse Umod embodies a substantial segment of the native protein. The inclusion of an N-terminal 6xHis-tag facilitates efficient purification and detection processes. This protein product showcases purity surpassing 90%, as established by SDS-PAGE, guaranteeing you a high-grade product for your research needs. The Recombinant Mouse Umod can be acquired in either liquid or lyophilized powder form, depending on your experimental and storage needs.

Target Background

Function

Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.; In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals.

Gene References into Functions

these data support THP as an important negative regulator of neutrophil activation in the urinary tract, with dual functions to counteract bacterial colonization and suppress excessive inflammation within the urinary tract PMID: 28829050
this study shows that uromodulin-SlpA binding dictates Lactobacillus acidophilus uptake by intestinal epithelial M cells PMID: 28992252
results strongly suggest that the progressive worsening of kidney functions reflects the accumulation of the deleterious effects of the misfolded mutant THP and the compensatory responses PMID: 29145399
Endoplasmic reticulum stress and apoptosis are key features in a mouse model of kidney disease driven by the uromodulin p.Cys147Trp mutation. PMID: 28990932
Consistently, pathway enrichment analysis indicates that mutant uromodulin expression affects ER function and protein homeostasis. PMID: 28437467
we show that Tamm-Horsfall protein deficiency stimulates proximal epithelial activation of the Interleukin-23/Interleukin-17 axis and systemic neutrophilia. PMID: 25556169
AGP was more effective in limiting hepatic inflammation and maintaining perfusion than saline or HAS, in both endotoxemic and septic mice. AGP sequestration of LPS may contribute to its anti-inflammatory effects. PMID: 23941548
UMOD upregulates TRPV5 by decreasing caveolin-1 dependent endocytosis of TRPV5. PMID: 23466996
UMOD regulates sodium uptake in the thick ascending limb of the loop of Henle by modulating the effect of tumor necrosis factor-alpha on NKCC2A expression, making UMOD an important determinant of blood pressure control. PMID: 24324041
Genetic susceptibility link to hypertension and chronic kidney disease to uromodulin expression and uromodulin's effect on salt reabsorption in the kidney. PMID: 24185693
Using an ischemia-reperfusion model of murine acute kidney injury, we show that, while THP expression in thick ascending loop is downregulated at the peak of injury, it is significantly upregulated 48 h after. PMID: 23389456
Quercetin has the uricosuric and nephroprotective actions mediated by regulating the expression levels of renal organic ion transporters and UMOD. PMID: 21909718
a unifying model that underscores the role of THP as a major regulator of renal and systemic immunity. PMID: 22451664
Molecular and cellular effects of Tamm-Horsfall protein mutations and their rescue by chemical chaperones. PMID: 22117067
Deletion of the von Hippel-Lindau protein (Vhl), which mediates HIF degradation under normoxia, using Tamm-Horsfall protein (Thp)-driven Cre expression showed strong expression of HIF-1alpha in thick ascending limb. PMID: 21921145
THP-null mouse may have a distal tubular defect and thus mimics ileostomy patients with low urine volumes and acidic urine. PMID: 21063698
THGP modulation of ROMK function confers a new role of THGP on renal ion transport and may contribute to salt wasting observed in FJHN/MCKD-2/GCKD patients. PMID: 21081491
These data clearly demonstrate a gain-of-toxic function of uromodulin mutations providing insights into the pathogenetic mechanism of the disease. PMID: 20472742
Normal Tamm-Horsfall protein (THP) plays an important role in defending the urinary system against calcification. Reduced expression and/or decreased function of THP could contribute to nephrolithiasis. PMID: 20591941
proximal 5'-flanking sequence is sufficient to drive the kidney-specific expression of a heterologous reporter gene PMID: 11880321
potential THP defects, either quantitative or qualitative, could predispose the urinary bladder to bacterial infections PMID: 14665435
THP serves as a soluble receptor for type 1 fimbriated E. coli and helps eliminate bacteria from the urinary tract. PMID: 14871399
THP is a critical urinary defense factor and its deficiency could be an important contributing factor in nephrolithiasis PMID: 15327412
Tamm-Horsfall glycoprotein plays an important regulatory role in the kidney PMID: 15522986
The genes encoding pre-pro-epidermal growth factor (pre-pro-EGF) and Tamm-Horsfall protein (THP)/uromodulin were aberrantly expressed in the kidneys of COX-2 -/- mice at all stages of their development. PMID: 15741608
Whole urine from osteopontin, Tamm-Horsfall protein , or double-null mice all possessed a dramatically reduced ability to inhibit the adhesion of calcium oxalate monohydrate crystals to renal epithelial cells PMID: 17898038
Urinary uromodulin is generated by a conserved C-terminal proteolytic cleavage and retains its entire ZP domain. PMID: 18375198
The finding that LZP might act as a new partner of THP would provide novel insights into renal functions related to THP and LZP, such as the urothelial permeability barrier and the host defense against the adhesion of pathogens. PMID: 18830570
Letter: Tamm-Horsfall protein knockout mice have increased stress induced micturition. PMID: 19214995
Umod might act as a gain-of-toxic-function mutation PMID: 19692485

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Subcellular Location

[Uromodulin, secreted form]: Secreted.; Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane.

Tissue Specificity

Detected in urine (secreted form). Detected in kidney thick ascending limb epithelial cells (at protein level).

Database Links

KEGG: mmu:22242

STRING: 10090.ENSMUSP00000033263

UniGene: Mm.10826

Code	CSB-YP025616MO
Abbreviation	Recombinant Mouse Umod protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Uromodulin (Umod), partial

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