Recombinant Mouse Versican core protein (Vcan), partial

The preparation of this recombinant Mouse Vcan protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a Vcan fragment (24-146aa) that could be translated into the Vcan protein and then transferred it into E.coli cells to express the recombinant Vcan protein molecule. In order to get the target protein with high purity, N-terminal 10xHis tag & C-terminal Myc tag was used in the production. The purity is 85% determined by SDS-PAGE.

VCAN, a large aggregating chondroitin sulfate proteoglycan belonging to the lexicon family, is an important extracellular matrix component, and is closely associated with tumorigenes. Four VCAN isoforms have been identified. The full-length isoform is V0, and the smaller isoforms V1, V2, and V3 result from alternative splicing. Previous studies have demonstrated that VCAN improves tumor cell survival, growth, migration, invasion, angiogenesis, and metastasis. Increased expression of VCAN has been reported in several malignancies, including leukemias as well as brain, colon, liver, prostate, breast, ovarian, oral squamous cell, and lung cancers, and is associated with adverse outcomes. A recent bioinformatic analysis revealed that VCAN may have an oncogenic role in GC, while another study suggested that VCAN expression predicts favorable outcomes in patients with GC.