Recombinant Mouse Vinculin (Vcl), partial

Code CSB-YP025812MO
MSDS
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Source Yeast
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Code CSB-EP025812MO
MSDS
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Source E.coli
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Code CSB-EP025812MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025812MO
MSDS
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Source Baculovirus
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Code CSB-MP025812MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vcl
Uniprot No.
Alternative Names
Vcl; Vinculin; Metavinculin
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Gene References into Functions
  1. Vinculin is necessary to maintain the integrity of the glomerular filtration barrier by modulating podocyte foot processes and stabilizing intercellular junctions. PMID: 29241625
  2. Vinculin-M94I resulted in 37% reduction in peak sodium current compared to wild type and the combination of Vinculin-M94I and pH 7.0 decreased peak sodium current. PMID: 28218286
  3. Lipid binding increases vinculin activation and turnover at focal adhesions. PMID: 28089450
  4. results suggest that the vinculin-actin interaction participates in FA-mediated mechanotransduction PMID: 28388663
  5. These results suggest that vinculin promotes the nuclear localization of transcription factor TAZ to inhibit the adipocyte differentiation on rigid extracellular matrix. PMID: 28115535
  6. These data demonstrate that Src-mediated phosphorylation is necessary for vinculin activation, and that phosphorylation controls cytoskeletal mechanics by regulating force transmission between the actin cytoskeleton and focal adhesion proteins. PMID: 26240176
  7. results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation. PMID: 26195589
  8. Force-dependent conformational switch of alpha-catenin controls vinculin binding. PMID: 25077739
  9. Mutant vinculin expressing cells are altered in cell migration, which is accompanied by changes in cell adhesion. PMID: 25358683
  10. show that Src phosphorylation of Y1065 within the C-terminal hairpin regulates Vt-mediated actin bundling and provide a detailed characterization of vinculin Y1065 mutations PMID: 25115937
  11. The E29R mutation might prime the vinculin head for F-actin binding, which results in higher cell stiffness, contractile force, and strengthening of focal adhesions. PMID: 25193699
  12. serine phosphorylation is required for the activation of vinculin and force transmission in focal adhesions. PMID: 24996180
  13. VCL binding talin, but not Arp2/3, is critical for osteoclast function, and its selective inhibition retards physiological bone loss. PMID: 24675074
  14. Crk-associated substrate -vinculin binding significantly affects the dynamics of Crk-associated substrate and vinculin within focal adhesions as well as the size of focal adhesions. PMID: 23974298
  15. This study demonistrated that Deletion of the vinculin disturbs the localization of GFAP in Bergmann glial cells in mice. PMID: 23595642
  16. These results identify vinculin as a major controller of integrin-mediated adhesion dynamics in B cells PMID: 23872053
  17. role for vinculin in ventricular myocyte mechanics and dysfunction PMID: 23561539
  18. Vinculin organizes lamellipodia and lamella F-actin and mediates F-actin retrograde flow engagement at focal adhesion to generate extracellular matrix traction. PMID: 23836933
  19. Data indicate that vinculin is not required for transmission of adhesive and traction forces but is necessary for myosin contractility-dependent adhesion strength and traction force and for the coupling of cell area and traction force. PMID: 23716647
  20. vinculin controls the transmission of intracellular and extracellular mechanical cues that are important for the spatiotemporal assembly, disassembly, and reorganization of focal adhesions to coordinate polarized cell motility PMID: 23375895
  21. alphaE-catenin recruits vinculin to adherens junctions more effectively than alphaN-catenin, partly because of its higher affinity for actin filaments. PMID: 23589308
  22. alpha-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength PMID: 23266828
  23. Results indicate a tissue-specific function of vinculin in cartilage whereby it controls chondrocytic differentiation. PMID: 22416133
  24. sca4 activates vinculin and interacts with the actin cytoskeleton; vinculin has roles in Rickettsia pathogenesis PMID: 21841197
  25. These results demonstrate that vinculin's mechano-coupling function is dependent on its conformational state. PMID: 21295550
  26. vinculin is not required for the traditional functions of alpha(IIb) beta(3) or the platelet actin cytoskeleton PMID: 20670372
  27. Data show that mutants of vinculin that are unable to interact with PtdIns(4,5)P, attenuate PIP5K- and LPA-induced neurite retraction. PMID: 20841379
  28. vinculin is an indispensable factor determining HSC repopulation capacity, independent of integrin functions. PMID: 20663867
  29. Vinculin-DeltaEx20 is an active variant supporting adhesion site stabilization without an enhanced mechanical coupling. Its presence in a transgenic animal reveals the potential of splice variants in the vinculin gene to alter vinculin function in vivo. PMID: 20644727
  30. phosphorylation on position Y1065 is essential for accurate incorporation of vinculin into FAs and mechanical behavior of cells. PMID: 20678470
  31. Vinculin facilitates cell invasion into three-dimensional collagen matrices. PMID: 20181946
  32. These results indicate that contractility modulates adhesion strengthening via FAK-dependent, vinculin-containing focal adhesion assembly. PMID: 20205236
  33. The phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. PMID: 20308429
  34. Both the lipid binding and the src-phosphorylation of vinculin's C-terminus are important for cell mechanical behavior. PMID: 20006947
  35. Vinculin found required for control of cell shape, cell mechanics, and rac-dependent lamellipodia formation PMID: 11785963
  36. Integrin-mediated adhesion does not require vinculin to couple with focal adhesion proteins for mechano-chemical signaling in embryonic F9 carcinoma cells. PMID: 11991656
  37. Vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction. PMID: 15138291
  38. model in which unfurling of the metavinculin tail domain is impaired by the negative charges of the 68-amino acid insert, thus requiring vinculin to fully activate the metavinculin molecule PMID: 15159399
  39. Wild-type vinculin and single tyrosine phosphorylation mutant proteins Y100F and Y1065F were significantly more effective at rescuing the spreading defect of vinculin null cells than the double mutant Y100F/Y1065F. PMID: 15229287
  40. Heterozygous inactivation of the vinculin gene predisposes to stress-induced cardiomyopathy PMID: 15331426
  41. studies indicate that PTEN protein levels are dependent on the maintenance of beta-catenin-MAGI-2 interaction, in which vinculin plays a critical role PMID: 15579911
  42. The Vinculin localized at costameres and intercalated discs. PMID: 15679099
  43. activated vinculin localized at focal adhesions recruits vinexins to focal adhesions PMID: 16126177
  44. The results suggest that vinculin inhibits cell migration by stabilising FAs, and that binding of inositol phospholipids to Vt plays an important role in FA turnover. PMID: 16584805
  45. This is the first report of tissue-specific inactivation of the Vcl gene and shows that it is required for preservation of normal cell-cell and cell-matrix adhesive structures. PMID: 17785437
  46. data demonstrate that vinculin contributes to the mechanical stability under large external forces by regulating contractile stress generation. PMID: 17890382
  47. The expression of vinculin constructs with unmasked binding sites in the head and tail regions induces dramatic focal adhesion growth, which is mediated by their direct interaction with talin. PMID: 18056416
  48. Abl differentially regulates Crk-associated substrate, vinculin, and paxillin in arterial vessels PMID: 19542491

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Vinculin/alpha-catenin family
Database Links
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