Recombinant Mouse Voltage-dependent L-type calcium channel subunit alpha-1D(Cacna1d) ,partial

Code CSB-YP860754MO
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Source Yeast
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Code CSB-EP860754MO
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Source E.coli
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Code CSB-EP860754MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP860754MO
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Source Baculovirus
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Code CSB-MP860754MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Cacna1d
Uniprot No. Q99246
Alternative Names Cacna1d; Cach3; Cacn4; Cacnl1a2; Cchl1a2; Voltage-dependent L-type calcium channel subunit alpha-1D; Calcium channel; L type; alpha-1 polypeptide isoform 2; Voltage-gated calcium channel subunit alpha Cav1.3
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Gene References into Functions
  1. The results signify a developmental decline in the sensitivity of CDI to global elevations in Ca ( 2+) , which restricts negative feedback regulation of Cav 1.3 channels to incoming Ca ( 2+) ions in mature inner hair cells (IHCs). PMID: 23510940
  2. Ventral tegmental area Cav1.3 channel activation robustly mediates cocaine-related, depression-related and social behavior via distinct nucleus accumbens AMPAR mechanisms with no effect on anxiety-like behavior. PMID: 28194001
  3. findings suggest a key role of CaV1.3 in regulating dendritic spine structure. Under physiological conditions it may contribute to the structural plasticity of glutamatergic synapses. Conversely, altered regulation of CaV1.3 channels may provide an important mechanism in the development of postsynaptic aberrations associated with neurodegenerative disorders. PMID: 27708393
  4. These results provide the evidence of a direct regulatory role of Snapin on Cav1.3 channels in atrial myocytes. PMID: 27915047
  5. Ca(2+) entry serves critical cellular functions in virtually every cell type, and appropriate regulation of Ca(2+) in neurons is essential for proper function. PMID: 28258168
  6. Auditory inner hair cells (IHCs) encode sounds into nerve impulses through fast and indefatigable Ca(2+)-dependent exocytosis at their ribbon synapses. We show that this synaptic process involves long and short C-terminal isoforms of the Cav1.3 Ca(2+) channel that differ in the kinetics of their Ca(2+)-dependent inactivation and their relative sensitivity to the l-type Ca(2+) channel blocker nifedipine. PMID: 28193694
  7. Inner hair cells possess calcium channels that are essential for transmitting sound information into synaptic transmitter release. Voltage-gated calcium channels can coassemble with auxiliary subunit alpha2delta isoforms 1-4. We found that hair cells of the mouse express the auxiliary subunit alpha2delta2, which is needed for normal hearing thresholds. Using a mouse model with a mutant, nonfunctional alpha2delta2 prote... PMID: 27798183
  8. alpha1D Ca and SK4 channels are coupled in the atria, and deletion of alpha1D leads to decreased SK4 mRNA and BNP secretion providing evidence for a novel role of alpha1D in atrial endocrine function PMID: 27884747
  9. beta-Adrenergic stimulation enhanced pacemaking of both genotypes, though, Cav1.3(-/-) sino-atrial node cells remained slower than wild type PMID: 26786159
  10. implicating Cav1.3 as an essential element for hippocampus-associated cognitive functions PMID: 26459417
  11. Studies indicate the function of L-type calcium channels Cav1.3 in chromaffin cells. PMID: 25966692
  12. Data suggest that the ion channels CaV1.3, bestrophin-1 and maxiK were identified as players in the regulation of photoreceptor outer segments (POS) phagocytosis by the retinal pigment epithelium (RPE). PMID: 26427483
  13. RIM2alpha and RIM2beta promote a large complement of synaptic CaV1.3 Ca(2+) channels at inner hair cell presynaptic active zones and are required for normal hearing. PMID: 26034270
  14. Results indicate that the Cav1.3 subtype is crucial to regulation of basal single-spike firing, while activation of both Cav1.2 and Cav1.3 can support burst firing of dopaminergic VTA neurons. PMID: 24848473
  15. ablation of Cav1.3 results in a decrease in the protein expression of myosin light chain 2, which interacts and increases the membrane localization of SK2 channels. PMID: 25538241
  16. Cav1.3 significantly upregulated in brain 24hr after single nicotine injection; Cav1.2 expression was not significantly altered; downregulation of Cav1.2 24hr after chronic nicotine administration; strong upregulation of Cav1.2 following 7-day abstinence PMID: 24470632
  17. CaV1.3(-/-) RPE lacked peak phagocytic activity following morning photoreceptor shedding in wild-type RPE and retained a higher number of phagosomes at a later time of day. PMID: 24407175
  18. (Cav1.3) The key yet differential roles of Cav1.2 and Cav1.3 channels mediate action potential firing and exocytotic events in the neuroendocrine chromaffin cell. PMID: 21054386
  19. Cav3.2 channels support dilatation of efferent arterioles and affect glomerular filtration rate, whereas Cav3.1 channels in vivo contribute to renal vascular resistance. PMID: 24966091
  20. The co-localization of Ca(v)1.3 and CB in certain brain regions such as the hippocampus suggests their important roles in neuroplasticity. PMID: 24394909
  21. Ca(v)1.3.3 calcium channel is a good target for prevention and therapy of age-related hearing loss PMID: 23470431
  22. Report role of Cav1.3 in controlling sinus rhythm. PMID: 23045342
  23. A Cav1.3-dependent mechanism is demonstrated through which both inhibitory circuit formation and determination of the neurotransmitter phenotype are achieved. PMID: 23077046
  24. The role of the peripheral deafness gene Cacna1d, was assessed. PMID: 22678062
  25. Findings indicated that the LTCC alpha(1D) subunit located on the ER membrane has a novel function as a gatekeeper for the Zn wave, which is involved in regulating NF-kappaB signaling and the delayed-type allergic reaction. PMID: 22745805
  26. our results neurochemically corroborate the robustness of the nigrostriatal DA neurons in the absence of Ca(v)1.3 channels, but suggest that complete deletion of this channel affected a variety of other transmitter systems PMID: 22202668
  27. analysis of functional characterization of alternative splicing in the C terminus of L-type CaV1.3 channels PMID: 21998309
  28. expression of L-VGCC alpha1D subunit found in WT hearts was abolished in clock mutant hearts, indicating that in mammalian hearts, the daily oscillations of the activities of L-VGCCs were downstream elements of cardiac core oscillators PMID: 21921295
  29. while Ca(v)1.3 channels are required for automaticity, Ca(v)3.1 channels are important for maximal pacing rates of mouse AVNCs. PMID: 21406960
  30. at potentials near resting (-50 mV), Ca(v)1.3 carries equal amounts of Ca(2+) current to Ca(v)1.2 but activates at 9 mV more negative potentials. PMID: 21406973
  31. The Cacna1d has been expressed in proliferating myoblasts, whereas in differentiating mononuclear myoblasts the expression of this gene was significantly decreased. PMID: 21789999
  32. results imply that Cav1.3 channels are indispensable for the development of the central auditory system. PMID: 21632949
  33. Cacna1d knockout mice are deaf and have impaired sinoatrial node function. PMID: 17650821
  34. Study demonstrates that the distinct biophysical properties of Ca(v)1.3 Ca(2+) channel play critical roles in the firing frequency of AV node tissues. PMID: 20951705
  35. L-type divalent calcium channels and their process development are in situ evidence for the influence of golli myelin basic proteins on voltage-operated channels, offering an explanation for the hypomyelination observed in golli-deficient mouse brains. PMID: 20607717
  36. findings indicate that CaV1.3 L-type Ca2+ channels modulate depression-like behaviour but are not essential for visual function. PMID: 19664321
  37. besides presumably reduced synaptic bodies the absence of class D L-type Ca(2+) channels does not prevent morphological development of the cochlea until postnatal day but may cause cochlear degeneration thereafter PMID: 12684182
  38. In outer hair cells from Cav1.3 deficient mice, Ba2+ current density was reduced to 0.6 +/- 0.5 pA pF-1 (n = 9) indicating that > 97 % of the Ca2+ channel current in OHCs flows through Cav1.3 PMID: 14514878
  39. Cav 1.3 (alpha1D) L-type Ca2+ channel in mouse is expressed in the liver PMID: 15010858
  40. During development, Ca(v)1.3 immunoreactivity was observed in the cochlea as early as embryonic day 15, with expression increasing at birth. PMID: 15158080
  41. In contrast to impaired hearing, alpha1D-/- mice have balance performances equivalent to their wild-type littermates PMID: 15357422
  42. D2 dopaminergic & M1 muscarinic receptors selectively modulate CaV1.3 in striatal medium spiny neurons. The splice variant in these cells has cytoplasmic Src homology 3 and PDZ-95/Discs large/ZO-1 domains that bind Shank. PMID: 15689540
  43. We established the differential distribution of Cav1.3 Ca2+ channels in atrial myocytes compared with ventricles. Our data represent the first report demonstrating important functional roles for Cav1.3 Ca2+ channel in atrial tissues. PMID: 16172271
  44. Cav1.3 knockout mice exhibit significant impairments in consolidation of contextual fear conditioning. Overtrained KO mice exhibit normal rates of extinction. Cav1.3 knockout mice perform normally on the hidden platform version of the Morris water maze. PMID: 17015855
  45. In conclusion, our results find that amphetamine increases D(2)L mRNA expression in the dStr and the VTA, an adaptation that correlates with expression of sensitized behavior and dependence on Ca(v)1.3 Ca(2+) channels. PMID: 17026527
  46. Voltage-sensitive Ca2+ channels Cav1 .2 and Cav1.3 are the predominant brain l-type calcium channels PMID: 17052224
  47. alternative splicing of alpha1 1.3 may influence activity-dependent regulation of Ca(v)1.3 channels at the synapse PMID: 17287512
  48. Our results identify Ca(V)1.3 as the principal pore-forming subunit of VDCCs involved in stimulating the ERG LP. PMID: 17376851
  49. L-type calcium channel Ca(v)1.3 (alpha1D) subunit mRNA in rods indicates it may be component of channels mediating residual photoreceptor responses observed in mutant mice lacking functional Ca(v)1.4 (alpha1F) subunits and in humans with CSNB2. PMID: 17563731
  50. The L-type channel Ca(v)1.3 protein significantly decreased upon chronic morphine treatment. PMID: 18048029

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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families Calcium channel alpha-1 subunit (TC 1.A.1.11) family, CACNA1D subfamily
Tissue Specificity Expressed in the inner hair cells (IHC) of the cochlea.
Database Links

KEGG: mmu:12289

STRING: 10090.ENSMUSP00000107869

UniGene: Mm.9772

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