Recombinant Mouse Voltage-dependent L-type calcium channel subunit beta-4 (Cacnb4)

Code CSB-YP819159MO
MSDS
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Source Yeast
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Code CSB-EP819159MO
MSDS
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Source E.coli
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Code CSB-EP819159MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819159MO
MSDS
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Source Baculovirus
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Code CSB-MP819159MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cacnb4
Uniprot No.
Alternative Names
Cacnb4; Cacnlb4Voltage-dependent L-type calcium channel subunit beta-4; CAB4; Calcium channel voltage-dependent subunit beta 4
Species
Mus musculus (Mouse)
Expression Region
1-519
Target Protein Sequence
MSSSYGKNGA ADGPHSPSSQ VARGTTTRRS RLKRSDGSTT STSFILRQGS ADSYTSRPSD SDVSLEEDRE AIRQEREQQA AIQLERAKSK PVAFAVKTNV SYCGALDEDV PVPSTAISFD AKDFLHIKEK YNNDWWIGRL VKEGCEIGFI PSPLRLENIR IQQEQKRGRF HGGKSSGNSS SSLGEMVSGT FRATPTTTAK QKQKVTEHIP PYDVVPSMRP VVLVGPSLKG YEVTDMMQKA LFDFLKHRFD GRISITRVTA DISLAKRSVL NNPSKRAIIE RSNTRSSLAE VQSEIERIFE LARSLQLVVL DADTINHPAQ LIKTSLAPII VHVKVSSPKV LQRLIKSRGK SQSKHLNVQL VAADKLAQCP PEMFDVILDE NQLEDACEHL GEYLEAYWRA THTSSSTPMT PLLGRNVGST ALSPYPTAIS GLQSQRMRHS NHSTENSPIE RRSLMTSDEN YHNERARKSR NRLSSSSQHS RDHYPLVEED YPDSYQDTYK PHRNRGSPGG CSHDSRHRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
Gene References into Functions
  1. Study demonstrates that the lack of the voltage-gated calcium channel beta4 subunit causes a decreased late development in cerebellar cortical networks, characterized by reduced parallel fibre-purkinje cell input, shorter purkinje cell dendrites and depressed purkinje cell output. PMID: 27003325
  2. The nuclear targeting properties of the truncated beta(4b(1-481)) subunit in tsA-201 cells, skeletal myotubes, and in hippocampal neurons, were investigated. PMID: 24875574
  3. an alternatively spliced beta4 subunit of voltage-gated calcium channels lacking the variable N terminus (beta4e) is identified PMID: 24453333
  4. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy PMID: 22892567
  5. Ca(v) beta(4) protein was detected throughout the developing cerebellum, particularly in the molecular layer, and in contrast to the other subunits, Ca(v) beta(4) was mainly detected in the molecular layer and the hilus of the hippocampus. PMID: 22737983
  6. The Ca2+ channel beta4c subunit interacts with heterochromatin protein 1 gama via a PXVXL binding motif. PMID: 21220418
  7. Cacnb4 is associated with acute lung injury PMID: 21297076
  8. analysis of calcium channel beta4b subunit nuclear targeting in nerve and muscle cells PMID: 19755859
  9. Paroxysmal dyskinesias as well as absence epilepsy, chronic ataxia, and hypoactivity noted in the lethargic mouse mutant with CCHB4 gene mutation PMID: 12223573

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Protein Families
Calcium channel beta subunit family
Database Links
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